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Citations for Zymo Research :
251 - 300 of 343 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Bioengineering 2020Quote: ... Around 50 ml of yeast culture was harvested and DNA was extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research). Two rounds of PCR were performed to amplify the coding sequence of the selected proteins and attach standard Illumina sequencing adaptors ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNAs were isolated using the Zyppy plasmid mini prep kit or ZymoPURE II plasmid maxiprep kit (Zymo Research, USA); recombinant plasmids were verified by restriction enzyme digestion and Sanger DNA sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids expressing eCFP-SGD and eGFP-MDR were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 □ ...
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bioRxiv - Immunology 2023Quote: ScFv encoding plasmids were recovered from yeast cultures by miniprep with the Zymoprep yeast plasmid miniprep II kit (Zymo Research) as previously described(42) ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Genetics 2024Quote: ... The library was transformed into yWS2542 (ram11 ydj11) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) per the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2023Quote: ... A maximum of 96 PCR reactions were pooled at a time and cleaned with Zymo-Spin II columns (Zymo Research). Pooled samples were sequenced with the NGSelect Amplicon sequencing service of Eurofins Genomics.
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bioRxiv - Cell Biology 2023Quote: MAGIC YKO was constructed with a two-step transformation using the Frozen-EZ Yeast Transformation II Kit (T2001, Zymo Research) following the microscale protocol in 96-well format ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Genetics 2023Quote: ... coli-derived Ydj1-CXXX plasmid library was transformed into yWS304 (ydj1Δ) via Frozen-EZ Yeast transformation II Kit by Zymo Research (Irvine ...
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bioRxiv - Synthetic Biology 2024Quote: ... Yeast transformation and plasmid DNA isolation were done with Frozen-EZ Yeast Transformation II kits and Zymoprep DNA isolation kit from Zymo Research ...
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bioRxiv - Synthetic Biology 2024Quote: ... the DNA was used to transform competent EBY-100 yeast with the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast cells were cultured in minimal glucose (SDCAA ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were isolated from fully grown cultures (1 mL) using a Zymoprep™ Yeast Plasmid Miniprep II kit (Zymo Research) according to the manufacturer’s protocol and the remaining culture was propagated to start the next screening round.
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids expressing GmIFS2-eCFP and GmCHS7 / GmCHIB1 / GmCHR5 / GmHID -eGFP were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Bioengineering 2022Quote: ... cerevisiae strain EBY100 (ATCC MYA-4941) (Boder & Wittrup, 1997) using frozen-EZ yeast transformation II kit (Zymo Research, Cat. No. T2001) and grown on SD-CAA agar plates for 3 to 4 days ...
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bioRxiv - Molecular Biology 2021Quote: ... one each with the insert in genomic and in antigenomic orientation for plasmid stock preparation using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2020Quote: ... Sequence verified and purified plasmids were transformed into yeast strain W303α using the Frozen-EZ Yeast Transformation II Kit (Zymo research) according to the manufacturer’s instructions and plated on YNB-URA dropout plates ...
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bioRxiv - Microbiology 2021Quote: ... were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research, USA) and following the manufacturer-supplied protocol and recommendations ...
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bioRxiv - Genomics 2021Quote: ... Clones were verified by Sanger sequencing (Quintara Biosciences) prior to retransformation and maxiprep using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Cell Biology 2022Quote: Testing and control combinations of plasmids were co-transformed into yeast AH109 strain using the Frozen-EZ Yeast Transformation II Kit (ZYMO, T2001) and cultured on a SD/-Leu-Trp selective plate at 30 °C for 2–4 days ...
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from yeast libraries using Zymoprep-96 Yeast Plasmid Miniprep kits or Zymoprep Yeast Plasmid Miniprep II kits (Zymo Research) according to standard manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from the yeast library using Zymoprep-96 Yeast Plasmid Miniprep kits or Zymoprep Yeast Plasmid Miniprep II kits (Zymo Research) according to standard manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: scFv encoding plasmids were recovered from yeast cultures by yeast miniprep with the Zymoprep yeast plasmid miniprep II kit (Zymo Research). Isolated DNA was transformed into NEB5α strain of E ...
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bioRxiv - Immunology 2021Quote: ... and enriched yeast plasmid libraries were extracted using Zymoprep Yeast Plasmid Miniprep II kit according to the manufacturer’s instructions (Zymo Research D2004).
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bioRxiv - Bioengineering 2023Quote: ... or a modified pNTA vector with V5 tag into EBY-100 yeast using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast were passaged once in minimal glucose medium (SDCAA ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Genomics 2023Quote: ... All colonies from each reaction were scraped from the plate and the CROP-seq-opti-Puro-T2A-GFP-sgRNA plasmid library was extracted using ZymoPURE II Plasmid Midiprep Kit (Zymo, D4200). The lentiviral library was generated as stated above with extended virus production time ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae strain BY4742 with endogenous Ycf1 knockout (Horizon Discovery) were transformed following the Frozen-EZ Yeast Transformation II protocol (Zymo Research). Transformed yeast strains were grown for 48 hours on YNB-His agar plates at 30 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB) + 50 μg/mL Ampicillin and purified using the ZymoPURE II Plasmid Midiprep kit (Zymo D4200) according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Synthetic Biology 2024Quote: ... colonies were scraped with 50 mL 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203). These plasmid libraries constitute the “post-selection libraries.”
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Biochemistry 2022Quote: ... and transformed into the EBY100 yeast surface display strain [14] that had been made chemically competent using the Frozen EZ-Yeast Transformation II Kit (Zymo Research). For each single site-directed mutant library transformed yeast were plated onto three each Synthetic Dropout minus Tryptophan (SD -Trp ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... yeast (PUP1/pup1Δ::kanMX) with a Magic marker selection were made using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For maximum representation of mutant human gene clones transformed in the strain ...
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bioRxiv - Plant Biology 2022Quote: Bait and prey constructs were co-transformed into the yeast strain AH109 using the yeast transformation kit (Frozen-EZ Yeast Transformation II Kit, Zymo Research). The resulting transformants with appropriate positive and negative controls were spotted on SD (−Leu ...
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bioRxiv - Physiology 2024Quote: ... was diluted in water and added directly to the column (50 μL for Zymo-II and 60 μL for Zymo-IICG). The column was sealed with a cap or parafilm and then incubated at 37°C for 45 min ...
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bioRxiv - Physiology 2024Quote: ... was diluted in water and added directly to the column (50 μL for Zymo-II and 60 μL for Zymo-IICG). The column was sealed with a cap or parafilm and then incubated at 37°C for 45 min ...