Labshake search
Citations for Zymo Research :
201 - 250 of 343 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Plasmids were isolated from expanded bacterial colonies using the ZymoPURE™ II Plasmid Kit (Zymo Research #D4203). Ten μg of isolated plasmid was transfected into HEK293T cells using Lipofectamine® 2000 Transfection Reagent (Invitrogen #11668-019) ...
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bioRxiv - Genomics 2022Quote: ... plasmid DNA was harvested from the 1L culture using the ZymoPURE II Plasmid Gigaprep (Zymo Research, #D4204). Before prepping ...
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bioRxiv - Microbiology 2022Quote: pAd/SARS-CoV-2-S1Beta was amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... and yeast plasmid DNA was extracted via plasmid DNA miniprep using Yeast Miniprep kit II (Zymo Research) then sequenced via whole plasmid Oxford nanopore sequencing to determine the successful expression cassette combinations.
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bioRxiv - Immunology 2024Quote: DNA was isolated from 5-10×10⁷ yeast per selection using a Zymoprep II kit (Zymo Research). Unique barcodes and random 8mer sequences were added to the sequencing product by PCR and amplified for 25 cycles to allow for downstream demultiplexing and improved clustering ...
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bioRxiv - Immunology 2024Quote: ... Plasmid DNA was extracted from these libraries using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). The antibody region was then PCR-amplified and diversified through EP PCR ...
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bioRxiv - Immunology 2024Quote: ... Plasmid DNA was purified from liquid bacterium cultures using the ZymoPure II Plasmid Maxiprep kit (Zymo Research). Lentivirus for modification of LLC cells was generated with a second-generation lentiviral packaging system (pMD2.G and pCMV-dR8.91 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and plasmids encoding protein designs were extracted using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were pelleted and lysed for DNA extraction using Zymoprep Yeast Plasmid Miniprep II (Zymo Research) using the manufacturers instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the plasmids were isolated using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Cat. No. D4201-B) according to the manufacturer’s instruction ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... ACE2 mutant yeast display plasmids were rescued using the ZymoPrep Yeast Plasmid Miniprep II kit (Zymo Research). Rescued plasmids were amplified via electroporation into 10G Supreme E ...
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bioRxiv - Bioengineering 2024Quote: ... plasmid DNA was extracted and purified from pellets over a ZymoPURE™ II Plasmid Gigaprep column (Zymo) and recovered in nuclease-free water ...
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bioRxiv - Genetics 2020Quote: ... ACE2 mutant library yeast display plasmids were rescued using the ZymoPrep Yeast Plasmid Miniprep II kit (Zymo Research). Rescued plasmids were amplified via electroporation into 10G Supreme E ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA was transformed into chemically competent EBY100 using the Frozen-EZ yeast transformation Kit II (Zymo Research). Trp-deficient SDCAA and SGCAA medium was used for culturing cells and for inducing cell surface protein expression for cells containing the pCTCON vector and Leu-deficient SDSCAA1 (-Leu ...
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bioRxiv - Immunology 2021Quote: ... Plasmids containing TCR libraries were purified using a ZymoPURE II Plasmid Maxiprep Kit (D4202, Zymo Research, Irvine, CA). The TRBC-p2A-TRAL insert was amplified using a KAPA HiFi HotStart PCR Kit (7958897001 ...
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bioRxiv - Immunology 2021Quote: ... plasmid DNA was isolated from the sorted yeast populations using a Zymoprep Yeast Miniprep II Kit (Zymo Research). Stellar cells (Takara ...
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bioRxiv - Synthetic Biology 2023Quote: ... Resultant plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Corresponding yeast strains were cultivated at 30□ ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Transformation was carried out by electroporation for libraries48 and using the Frozen-EZ Yeast Transformation II Kit (Zymo) for individual constructs.
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bioRxiv - Microbiology 2023Quote: ... Competent yeast cells were prepared and transformed with the Frozen-EZ Yeast Transformation II Kit (Zymo Research, USA), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... high titer plasmid DNA was isolated using a ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, Irvine, USA) and used for transfection of mammalian cells utilized in the functional heterologous receptor assay.
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bioRxiv - Synthetic Biology 2023Quote: ... Competent LLE cells were then created using the Zymo Research Frozen-EZ Yeast Transformation II Kit (Zymo#T2001). When the component cells containing either Integrated TEVsubstrate or TEVp were complete ...
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bioRxiv - Genetics 2024Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). The coding sequences of TabHLH27-A1 were cloned into the prey vector (pGADT7) ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). For the interaction of TaNF-YA3-D ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
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bioRxiv - Genomics 2024Quote: ... pellets were thawed and plasmids were isolated using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research, D2004) with minor modifications ...
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bioRxiv - Bioengineering 2024Quote: ... correct plasmid clones were further expanded and isolated using the ZymoPure II Plasmid MaxiPrep Kit (Zymo Research; D4203).
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bioRxiv - Biochemistry 2024Quote: Plasmids from the transformed yeast cells were extracted using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: Plasmids from the sorted yeast cells were extracted using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Resultant plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Corresponding yeast strains were cultivated at 30℃ ...
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bioRxiv - Microbiology 2021Quote: ... The resulting plasmid was extracted from yeast using the Zymoprep yeast miniprep kit version II (Zymo Research, California, USA), amplified in E ...
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bioRxiv - Microbiology 2022Quote: ... (Hayashi et al., 2009): DL70 cells were made chemically competent (Frozen-EZ Yeast Transformation II Kit, Zymo Research, #T2001) and were transformed with a linear DNA fragment consisting of the mScarlet-I sequence and a hygromycin resistance cassette flanked by 500-600 bp of homology arms on both sides for homologous recombination into the genome downstream of the native sad1 gene ...
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bioRxiv - Genomics 2021Quote: ... and plasmids from both before and after selection were isolated using the Yeast Plasmid Miniprep II Kit (Zymo Research).
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bioRxiv - Genomics 2021Quote: Competent cells for each yeast strain were prepared using the Frozen-EZ Yeast Transformation II Kit™ (Zymo Research) according to the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2023Quote: ... These cells were cultured in LB media and plasmids were purified using ZymoPURE II Plasmid Midiprep kit (Zymo Research). Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... For GFP2 and GFP4 vectors successfully cloned enhancer constructs were further isolated with ZymoPure II Maxiprep kit (Zymo research).
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bioRxiv - Microbiology 2022Quote: ... and two community controls (ZymoBIOMICS Microbial Community DNA Standard and ZymoBIOMICS Microbial Community DNA Standard II (Log Distribution) (Zymo Research ...
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bioRxiv - Bioengineering 2022Quote: DNA of the active population was extracted from yeast cells using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) and amplified in two steps ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004). VH sequences were amplified by PCR and submitted to Genewiz for Amplicon NGS sequencing.
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bioRxiv - Synthetic Biology 2023Quote: ... and sequence confirmed (Sequetech) and then maxi-prepped per the manufacturer’s protocols (ZymoPure II Plasmid Maxiprep Kit, Zymo Research). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4 °C.
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bioRxiv - Synthetic Biology 2024Quote: ... Colonies were scraped with 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203); the extracted plasmid DNA was then precipitated with isopropanol ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Biochemistry 2022Quote: ... into the Saccharomyces cerevisiae wild-type strain CEN.PK2-1D using the Frozen-EZ yeast transformation II kit (Zymo Research) according to according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified for transfection into mammalian cell lines using a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Microbiology 2024Quote: ... Replicon DNA was isolated after another 6-hour growth using the ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research). Reporter replicon-transfected HEK293T cells were imaged 48 h after transfection on an EVOS M5000 Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
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bioRxiv - Developmental Biology 2020Quote: Combinations of the AD or BD vectors were co-transformed into yeast using the Frozen-EZ II kit from Zymo Research and using empty vectors as negative controls ...
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bioRxiv - Biochemistry 2021Quote: ... Quick-RNA miniprep Kit (Cat. No. R1054) and ZymoPURE II Plasmid Maxiprep Kit (Cat. No. D4203) were obtained from Zymo Research ...
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bioRxiv - Pathology 2021Quote: ... cerevisiae strains PLY240 (MATa his3Δ200 leu2-3,112 trp1Δ901 ura3-52 suc2Δ9 trk1Δ51 trk2Δ50::lox-kanMX-lox) [55] was transformed with the constructed pYES2sh plasmids using Frozen-EZ Yeast Transformation II kit (Zymo Research Europe GmbH ...
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bioRxiv - Microbiology 2021Quote: ... used in this study has a log abundance distribution of different bacterial species and fungi ranging from 108 to 102 cells (ZymoBIOMICS Microbial Community Standard II, ZYMO RESEARCH EUROPE GMBH ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection-grade plasmid DNA was isolated from each flask using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...