Labshake search
Citations for Zymo Research :
201 - 250 of 270 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Transfections were performed with PEI and 2-5 µg of transfection grade DNA (ZymoPure Plasmid MaxiPrep Kit, Zymo) per dish as described in above ...
-
bioRxiv - Plant Biology 2021Quote: ... 500 pg - 2 µg DNA was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research). Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1 ...
-
bioRxiv - Plant Biology 2024Quote: ... 10 µg of RNA were further cleaned up by RNA Clean & Concentrator-5 (Zymo Research, Irvine, USA; R1016). 500 ng of the purified RNA was employed to synthesize cDNA with the iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 500 ng of genomic DNA from cells in co-cultures were bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA). The Infinium HD Methylation Assay (bisulfite modification ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primer specificity was tested by performing ddPCR in the presence of an excess of eukaryotic (fish) or prokaryotic [ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA)] DNA ...
-
bioRxiv - Microbiology 2023Quote: ... DNA extraction was also performed on three no-sample added (negative) and three ZymoBIOMICS Microbial Community Standard (positive) controls (Zymo Research, Irvine, CA, USA). DNA from an additional eight GWSS had been previously extracted using the Blood and Tissue kit (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
-
bioRxiv - Microbiology 2021Quote: G RNAs were isolated from the viral samples using Direct-zol™ RNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... DNA-free viral RNA was isolated from the viral supernatants using Direct-Zol RNA Microprep kit (Zymo research) according to manufacturer’s instruction ...
-
bioRxiv - Genomics 2023Quote: ... RNA isolates were treated with DNase1 and cleaned using the DNA-free RNA Kit (Zymo Technologies, Irvine, CA). RNA quality was assessed by use of a fragment analyser (Advanced Analytical Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... PBS cell suspension was pipetted to a pre-chilled to 1.5 mL RNase-free snap lid tube (Zymo Research ...
-
bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
-
bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
-
bioRxiv - Microbiology 2024Quote: ... 75ng of DNA isolated using both lab types (n=6) and the Zymobiomics microbial community standard was used as a control (n=1) (Zymo Research Corporation, Irvine, CA, USA). Sequencing of all samples was run for 24 hours ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were then arrested in G1 using a final concentration of 4.0 µg/mL alpha-factor mating pheromone (Zymo Research) and incubated at 30°C with shaking until the vast majority of cells in the population exhibited the ‘shmoo’ phenotype (2.5 hours) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The solution was diluted to 10% RNALater by the addition of 270μL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA pellets were resuspended in RNAse-free water and digested with DNAseI for 20 min at room-temperature (Zymo). DNAseI-treated RNA was purified with a RNA-clean&concentrator-25 kit (Zymo) ...
-
bioRxiv - Neuroscience 2023Quote: ... Free RNAs were extracted using phenol/chloroform and purified with the RNA Clean & Concentrator-5 kit (Zymo Research, R1015).
-
bioRxiv - Bioengineering 2023Quote: ... Triplicate RNA samples from each condition were diluted to 20 ng/μl with DNase/RNase-Free Water (ZYMO RESEARCH). 15μl RNA solution of each replicate were sent to the UCLA Center for Systems Biomedicine for RNA expression assay with the Nanostring™ nCounter ...
-
bioRxiv - Microbiology 2024Quote: ... RNA was purified from cell-free viral preparations using the Direct-zol RNA Miniprep kit (Zymo Research catalog #R2052) according to manufacturer instructions ...
-
bioRxiv - Genomics 2024Quote: ... RNA pellets were resuspended in RNAse-free water and digested with DNAseI for 20 min at room-temperature (Zymo). DNAseI-treated RNA was purified with an RNA-clean&concentrator-25 kit (Zymo) ...
-
bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
-
bioRxiv - Microbiology 2022Quote: ... a negative control to account for microbial contaminants present in DNA extraction kits or introduced during the extraction (Eisenhofer et al., 2019) and a microbial community standard (Zymo Research, cat. no. D6300, Irving, CA). The DNA concentration was quantified with Qubit 3 using the high sensitivity dsDNA kit (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... Three sections from each sample were individually placed into Zymo Quick-DNA Fecal/Soil Microbe ZR BashingBead™ Lysis Tubes filled with DNA/RNA Shield™ (Zymo Research Co, Irvine, CA, United States). Homogenization and physical lysis of biomass samples was achieved in the field using a custom tube holder attached to a reciprocating saw ...
-
bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
-
bioRxiv - Microbiology 2022Quote: ... coli TOP10 cells were propagated in LB medium (ampicillin, 100 µg/ml) and used for recombinant plasmid DNA isolation (Zypy Plasmid Miniprep, Zymo Research). Prior to plasmid transformation to expression host E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The solution was diluted to 10% RNALater by the addition of 270 µL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
-
bioRxiv - Microbiology 2022Quote: ... total RNA was eluted from the column in 60 uL of Buffer AVE (QIAmp) or from the beads in 30 uL of DNase/RNase-free water (Zymo) and immediately used for RT-qPCR and library preparation ...
-
bioRxiv - Neuroscience 2023Quote: ... and RNA was isolated by miRNeasy Mini Kit solutions (Qiagen217004) and Zymo-Spin IIICG Columns (Zymo Research C1006-50-G) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
-
bioRxiv - Biochemistry 2021Quote: ... the RNA volume was brought up to 100 μL with nuclease-free water and was purified away from other reaction components by an RNA Clean & Concentrator kit (Zymo Research) by following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Samples were re-suspended with 50 μL of nuclease-free water and subsequently purified using the RNA Clean & Concentrator kit (Zymo, R1018). The concentration of each sample was determined using a Nanodrop spectrophotometer ...
-
bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). The Bxb1 recombinase expression vector (pCAG-NLS-HA Bxb1 ...
-
bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA extraction product was diluted 20-fold in DNase/RNase free water (from Zymo Research Direct-zol™ RNA MiniPrep Kit) before recording the UV absorbance spectra on a Hewlett Packard 8452 Diode Array Spectrophotometer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... we resuspended the pellet in 200 µL of nuclease-free water followed by addition of 750 µL of BashingBead buffer from the Direct-Zol RNA MicroPrep RNA kit (Zymo Research). We then transferred our 950 µL of suspension into BashingBead tubes ...
-
bioRxiv - Genomics 2024Quote: ... DNA was eluted in 105 µL RNAse free H2O and was further cleaned using OneStep PCR Inhibitor Removal Kit (Zymo Research). Concentrations for all DNA samples were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA from whole embryos or isolated tailbuds prepared as described above was treated with RNase-free DNase I (Zymo Research) to remove genomic DNA ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants for each sample were carefully transferred to RNase-free tubes for purification by the Direct-Zol RNA Miniprep Plus RNA purification kit (Zymo Research), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). Transient expression of individual variants for western blots was carried out using a distinct set of expression vectors encoding the untagged CFTR variant cDNAs in the context of a pCDNA5 expression vector ...
-
bioRxiv - Genomics 2024Quote: ... Total RNA (∼700 ng) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
-
bioRxiv - Cell Biology 2024Quote: ... 25 µl of RNase free water was added to the mixture and transcribed RNA was eluted using the RNA Clean & Concentrator kit (Zymo Research). Samples were assessed for their length and concentration by Bioanalyzer (Agilent ...
-
bioRxiv - Physiology 2021Quote: Hemolymph samples (n=112) were centrifuged for 10 minutes at 14000 g with RNA extracted using Quick DNA/RNA Microprep Plus Kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
-
bioRxiv - Physiology 2024Quote: ... were manually dissected out from the slices using 27 G needles and placed immediately into RNA lysis buffer from the Quick-RNA Microprep Kit (Zymo, R1050). Total RNA was isolated from tissue using the Quick-RNA Microprep Kit (Zymo ...
-
bioRxiv - Immunology 2024Quote: ... Samples were clarified by centrifugation at ∼21,000 x g and supernatant RNA was isolated by Quick-RNA Viral Kit (Zymo Research #R1034). RNA was quantified by NanoDrop™ 2000c (ThermoFisher) ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were verified by Sanger sequencing and purified with the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA). Two micrograms of plasmid DNAs were used to transfect small-scale HEKT293 cultures and optimize expression conditions both via mRuby2 visualization and western blot analysis ...
-
bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...