Labshake search
Citations for Zymo Research :
151 - 200 of 270 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... from weight-normalized contents from the distal small intestine and proximal colon after addition of Low Abundance Microbiota Standard (Zymo) and extraction using the Zymo DNA Mini kit (Zymo) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: Genomic (g)DNA was extracted using the Quick DNA miniprep kit (Zymo Research D3025) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was eventually eluted in 25 μL of DNase/RNase-free water (Zymo Research). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: ... Synthesized RNA was diluted 20-fold in nuclease-free water and purified (R1013, Zymo Research). The purified RNA (20 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... and prepared as endotoxin-free midipreps using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research)
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction reagent only controls were included for each batch of DNA extractions along with ZymoBIOMICS Microbial Community Standards (Zymo Research) and E ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from fecal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... Negative controls (sterile water treated as a sample) and one positive control (ZymoBIOMICS Microbial Community Standard, Zymo Research, Cat No. D6300) were included in each batch ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of DNA from the validation cohort of 20 patients (20 adenoma and 20 nontumor tissue samples) was processed with the EZ DNA Methylation-Gold Kit following the standard protocol (Zymo Research). Bisulfite conversion was followed by amplification of the target CpGs ...
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bioRxiv - Cancer Biology 2024Quote: ... 80-250 µg genomic DNA was bisulfite converted using the EZ DNA-methylation Kit (Zymo Research) and processed on Infinium MethylationEPIC BeadChip arrays (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 in DRG co-culture and 2 biological replicates of MDA-MB-231 in DRG-conditioned media (1 replicate was excluded during analysis after clustering revealed it was an outlier) was extracted 24h after co-culture using RNA MiniPrep Kit (R1057, Zymo Research, Irvine, CA). Samples were confirmed to have RIN value >8.5 with Agilent Bioanalyzer before undergoing library preparation with TruSeq stranded mRNA kit by Tufts Genomics Core ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast cells in mid-log phase were co-transformed with pDEST32/pDEST22::AtNHR2B or pDEST32::HopG1/ pDEST22::AtNHR2B using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). Transformed yeast cells were plated on Double Drop Out (DDO ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... using the manufacturer’s instructions or co-extraction of DNA and RNA was accomplished with a Quick DNA/RNA MiniPrep Plus Kit (Zymo Research, Irvine, CA). The quality and quantity of DNA and RNA were analyzed by either NanoDrop 1000 or NanoDrop One (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Genomics 2023Quote: Reads were aligned to the 16S genes extracted from the complete reference genomes of the bacteria present in the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) using minimap2 ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 10 trunk fragments per biological replicate using the standard Trizol RNA extraction protocol with high salt precipitation and clean-up on Zymo RNA columns (Zymo R1013/R1017). RNA (1 μg ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The inserts were verified with PCR and Sanger sequencing and then transformed into the yeast hosts using either the standard LiAc protocol (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and plated onto SD-leu media.
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were then transformed into this yeast strain using the standard LiAc method (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and selected for on SD-trp ...
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bioRxiv - Microbiology 2024Quote: ... The assembled plasmids were confirmed by standard yeast colony PCR and then extracted from yeast using the Zymoprep Yeast Plasmid Miniprep I (Zymo Research, USA). Selected plasmids were used for E ...
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bioRxiv - Microbiology 2024Quote: ... Positive controls were spiked with 75 µl of the standard microbial community II from Zymobiomics (Cat. No. D6310, Zymo Research Corporation, USA), hereafter referred to as “mock” ...
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bioRxiv - Neuroscience 2024Quote: ... was used as a positive control for each DNA extraction and the ZymoBIOMICS® Microbial Community DNA Standard (Zymo Research, Irvine, CA) was used as a positive control for each targeted library preparation ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from brain and spinal cord was isolated using the standard TriZol protocol followed by purification using the Direct-zol RNA Miniprep or Microprep kit (Zymo Research R2052). The concentration of RNA samples was determined using a NanoDrop One (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Staphylococcus aureus, Listeria monocytogenes, Bacillus subtilis) and two yeast species (Saccharomyces cerevisiae, Cryptococcus neoformans) (ZymoBIOMICS Microbial community DNA standard, Zymo Research, Germany). The mock community and the positive control provided by the company were replicated twice ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... Endotoxin-free plasmid library DNA was prepared using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research). To clone the library of gRNA pairs ...
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bioRxiv - Microbiology 2024Quote: ... The DNA-free RNA was cleaned up with the RNA Clean & Concentrator-5 kit (Zymo Research) and eluted in 15 µl of water ...
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bioRxiv - Cancer Biology 2020Quote: ... Bisulfite conversion of 1 µg genomic DNA was performed using EZ DNA Methylation Kit (D5001, Zymo Research). The bisulfite-converted DNA samples were subject to genome-wide methylation analysis using the Illumina Infinium MethylationEPIC BeadChips ...
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bioRxiv - Genomics 2022Quote: ... we co-extracted DNA and RNA in parallel using a modified version of the Quick-DNA/RNA Microprep Plus Kit (Zymo Research; Irvine; CA U.S.A.). DNA and RNA library prep and sequencing were performed by a service provider (Génome Québec ...
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bioRxiv - Microbiology 2021Quote: ... buffer without any added fecal material) and two microbial mock communities as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, California, USA).
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Genomics 2024Quote: ... two C57BL/6 wild-type murine cecal samples (generated previously in [18]) and one mock community (ZymoBIOMICS Gut Microbiome Standard, Zymo Research, Irvine, CA) were used to test the efficacy of the primer sets across multiple sample types ...
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bioRxiv - Genomics 2022Quote: ... take out 10 μl and add 40 μl nuclease-free for second column purification (Zymo Research, R1016) followed by elution with 10 μl nuclease-free water ...
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bioRxiv - Genomics 2020Quote: ... Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation) from 50 mL Mach1 (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... All plasmids were prepared with the Endotoxin-Free Zymopure Midiprep or Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Genomics 2022Quote: ... total RNA was isolated in RNase-free water using Zymoclean RNA Clean & Concentrator™ (Zymo Research Corp.). Filtered high-quality reads were aligned to C ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were prepared for injection using ZymoPURE II endotoxin-free midiprep kit (Zymo Research Corp, Irvine, CA). Cas9/sgRNA and donor plasmids were diluted to concentrations of 300ng/μl for injection ...