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Citations for Zymo Research :
201 - 250 of 1848 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... (Hayashi et al., 2009): DL70 cells were made chemically competent (Frozen-EZ Yeast Transformation II Kit, Zymo Research, #T2001) and were transformed with a linear DNA fragment consisting of the mScarlet-I sequence and a hygromycin resistance cassette flanked by 500-600 bp of homology arms on both sides for homologous recombination into the genome downstream of the native sad1 gene ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Biochemistry 2022Quote: ... into the Saccharomyces cerevisiae wild-type strain CEN.PK2-1D using the Frozen-EZ yeast transformation II kit (Zymo Research) according to according to the manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... cerevisiae strains PLY240 (MATa his3Δ200 leu2-3,112 trp1Δ901 ura3-52 suc2Δ9 trk1Δ51 trk2Δ50::lox-kanMX-lox) [55] was transformed with the constructed pYES2sh plasmids using Frozen-EZ Yeast Transformation II kit (Zymo Research Europe GmbH ...
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Genetics 2024Quote: ... The library was transformed into yWS2542 (ram11 ydj11) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) per the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids expressing eCFP-SGD and eGFP-MDR were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 □ ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Genetics 2023Quote: ... coli-derived Ydj1-CXXX plasmid library was transformed into yWS304 (ydj1Δ) via Frozen-EZ Yeast transformation II Kit by Zymo Research (Irvine ...
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bioRxiv - Cell Biology 2023Quote: MAGIC YKO was constructed with a two-step transformation using the Frozen-EZ Yeast Transformation II Kit (T2001, Zymo Research) following the microscale protocol in 96-well format ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids expressing GmIFS2-eCFP and GmCHS7 / GmCHIB1 / GmCHR5 / GmHID -eGFP were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Bioengineering 2022Quote: ... cerevisiae strain EBY100 (ATCC MYA-4941) (Boder & Wittrup, 1997) using frozen-EZ yeast transformation II kit (Zymo Research, Cat. No. T2001) and grown on SD-CAA agar plates for 3 to 4 days ...
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bioRxiv - Microbiology 2021Quote: ... were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research, USA) and following the manufacturer-supplied protocol and recommendations ...
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae strain BY4742 with endogenous Ycf1 knockout (Horizon Discovery) were transformed following the Frozen-EZ Yeast Transformation II protocol (Zymo Research). Transformed yeast strains were grown for 48 hours on YNB-His agar plates at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Biochemistry 2024Quote: ... contains the Arabidopsis thaliana cytochrome P450 reductase I gene, ATR1) (Urban et al., 1997) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For plant transient expression ...
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bioRxiv - Plant Biology 2024Quote: ... Final GBKT7 plasmids was transformed into the yeast strain Y2HGold and transformants selected on SD medium lacking tryptophan (Trp) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Quantitative transactivation tests were performed as previously described (Ref) ...
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bioRxiv - Molecular Biology 2024Quote: ... The isolated yeast populations obtained after round 4 were subjected to colony screening and plasmid recovery using a Zymoprep yeast plasmid kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2024Quote: ... the collected yeast cells were grown to saturation and 1 ml of saturated culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit according to manufacturer protocols (using 10 µl zymolyase) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Wild type 4D5 and 5A12 VH and VL scFv gene fragments were purchased from Twist Bioscience and transformed into the BamHI digested pYD1 plasmid via homologous recombination (Boder and Wittrup, 1997; Chao et al., 2006) using Frozen-EZ Yeast Transformation II (Zymo Research, T2001). 4D5 scFv was transformed in a HA-VL-218-VH-FLAG orientation and 5A12 scFv as HA-VH-218-VL-FLAG ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Staphylococcus aureus, Listeria monocytogenes, Bacillus subtilis) and two yeast species (Saccharomyces cerevisiae, Cryptococcus neoformans) (ZymoBIOMICS Microbial community DNA standard, Zymo Research, Germany). The mock community and the positive control provided by the company were replicated twice ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast cells in mid-log phase were co-transformed with pDEST32/pDEST22::AtNHR2B or pDEST32::HopG1/ pDEST22::AtNHR2B using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). Transformed yeast cells were plated on Double Drop Out (DDO ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA of yeast colonies was isolated using the LiAc-sodium dodecyl sulfate method (54) or the YeaStar Genomic DNA kit (Zymo Research, Irvine, CA, USA). DNA fragments obtained by PCR were separated by gel electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA). Purified yeast-extracted DNA plasmid was then used as template to generate amplicons through two consecutive PCR reactions ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA constructs encoding single macrocyclic peptide ligand were verified by Sanger sequencing and further used to transform new competent EBY100 cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA, USA).
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Amplicons for next generation sequencing (NGS ...
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bioRxiv - Microbiology 2020Quote: ... reads from Zymo samples profiled with Metaphlan2 ...
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bioRxiv - Microbiology 2020Quote: ... reads from Zymo samples were aligned to the provided reference genomes using Bowtie 2 ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from sorted cells using a Micro kit from Zymo. Following DNA extraction and quantification with NanoDrop ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted from 1 mL of culture using kit D7005 from Zymo Research ...
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bioRxiv - Physiology 2023Quote: Total RNAs were extracted from cells and tissues employing the MiniPrep kit from ZYMO RESEARCH ...
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bioRxiv - Microbiology 2020Quote: ... is commercially available from Zymo Research (Zymo Research ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli Transformation Kit from Zymo Research (Irvine ...
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bioRxiv - Microbiology 2024Quote: ... we used kits from Zymo Research ...
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bioRxiv - Genomics 2024Quote: ... which are available from Zymo Research ...
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bioRxiv - Microbiology 2020Quote: Total RNAs were isolated from the whole blood samples from zebra finches and serum samples from woodchucks using Quick RNA Viral Kit (Zymo Research; Irvine, CA, USA). The obtained RNA samples were stored at −80°C until further requirement ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from samples using the ZymoBIOMICS Fecal/Soil DNA 96 Kit from Zymo Research (D6011 ...