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Citations for Zymo Research :
101 - 150 of 1848 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and enriched yeast plasmid libraries were extracted using Zymoprep Yeast Plasmid Miniprep II kit according to the manufacturer’s instructions (Zymo Research D2004).
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bioRxiv - Bioengineering 2023Quote: ... or a modified pNTA vector with V5 tag into EBY-100 yeast using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast were passaged once in minimal glucose medium (SDCAA ...
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Biochemistry 2022Quote: ... and transformed into the EBY100 yeast surface display strain [14] that had been made chemically competent using the Frozen EZ-Yeast Transformation II Kit (Zymo Research). For each single site-directed mutant library transformed yeast were plated onto three each Synthetic Dropout minus Tryptophan (SD -Trp ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... yeast (PUP1/pup1Δ::kanMX) with a Magic marker selection were made using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For maximum representation of mutant human gene clones transformed in the strain ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Immunology 2024Quote: ... The obtained yeast population was overnight amplified and collected for plasmid extraction using Zymoprep™ Yeast Plasmid Miniprep II (ZYMO RESEARCH). The N26 barcode was PCR amplified using the above extracted plasmid as the template and final PCR products were purified by Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2024Quote: ... complete medium was transformed with the yeast-display plasmid pCTCON2 (24) using the Frozen E-Z Yeast Transformation II kit (Zymo Research) according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... and plasmids from both before and after selection were isolated using the Yeast Plasmid Miniprep II Kit (Zymo Research).
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bioRxiv - Biochemistry 2024Quote: ... DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004). VH sequences were amplified by PCR and submitted to Genewiz for Amplicon NGS sequencing.
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bioRxiv - Biochemistry 2022Quote: Total RNA was isolated from ∼1.5 X 107 yeast cells using Quick-RNA Fungal/Bacterial Microprep Kit (Zymo Research). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Bioengineering 2023Quote: Plasmids of enriched nanobodies were isolated from the terminal round of sorting using a yeast miniprep kit (Zymo, D2004). For nanobody Fc fusions ...
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bioRxiv - Biochemistry 2024Quote: ... recovery of laccase-containing plasmids from these cultures was performed using Zymoprep Yeast Plasmid Miniprep Kit (Zymo research, #D2001) regarding the manufactureŕs specifications ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast expression vector constructs or empty vector were transformed into yeast Saccharomyces cerevisiae WAT11 (Pompon et al., 1996; Truan et al., 1993) by using Yeast Transformation II Kit (ZYMO, CA, USA) and screened on synthetic SD-dropout medium lacking uracil (SD-Ura ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The inserts were verified with PCR and Sanger sequencing and then transformed into the yeast hosts using either the standard LiAc protocol (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and plated onto SD-leu media.
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were then transformed into this yeast strain using the standard LiAc method (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and selected for on SD-trp ...
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bioRxiv - Cell Biology 2020Quote: ... of Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research). Yeast plasmid DNA was heated at 65 °C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast spheroplasts were generated using zymolyase digestion (Zymo Research) and spheroplast bursting due to osmotic shock measured using a Cary 60 UV-VIS (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae using a yeast plasmid miniprep kit (Zymo Research) before transformation into NEB®5-alpha competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... respectively using Frozen-EZ Yeast Transformation IITM (Zymo Research) as per the manufacturer’s protocol and plated on YNB (yeast nitrogen base ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) were used for bacterial and yeast cultures ...
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bioRxiv - Bioengineering 2023Quote: ... Individual yeast plasmids were extracted via miniprep (Zymo Research) to verify proper insertion of the fragment into the vector backbone ...
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bioRxiv - Developmental Biology 2020Quote: Combinations of the AD or BD vectors were co-transformed into yeast using the Frozen-EZ II kit from Zymo Research and using empty vectors as negative controls ...
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bioRxiv - Synthetic Biology 2024Quote: ... Yeast transformation and plasmid DNA isolation were done with Frozen-EZ Yeast Transformation II kits and Zymoprep DNA isolation kit from Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were isolated from fully grown cultures (1 mL) using a Zymoprep™ Yeast Plasmid Miniprep II kit (Zymo Research) according to the manufacturer’s protocol and the remaining culture was propagated to start the next screening round.
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bioRxiv - Bioengineering 2022Quote: Yeast competent cells were transformed with polycistronic constructs containing fluorescent proteins using the Frozen-EZ yeast transformation II kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA plasmid present within the yeast cells was extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Purified yeast-extracted DNA plasmid was then used to transform competent E ...
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bioRxiv - Genetics 2022Quote: ... coli using the Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research). Competent cells were prepared using the Inoue method [17].
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bioRxiv - Biochemistry 2021Quote: ... using a Zymo Yeast Plasmid Miniprep II kit (Zymo Research) and a Monarch PCR & DNA Cleanup kit (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... using the Frozen-EZ Yeast Transformation II Kit (Zymo Research) and the transformants were grown on the solid SD media lacking Leu and Trp (SD-LW) ...
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bioRxiv - Microbiology 2022Quote: ... glabrata cells using Frozen-EZ Yeast Transformation Kit (Zymo Research) and transformation followed an electroporation-based protocol described previously (65) ...
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bioRxiv - Bioengineering 2024Quote: ... using a Zymo Yeast Plasmid Miniprep II kit (Zymo Research) and a Monarch PCR & DNA Cleanup kit (NEB ...
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bioRxiv - Plant Biology 2024Quote: A Zymoprep™ Yeast Plasmid Miniprep II kit (Zymo Research) was used to recover plasmid DNA from Saccharomyces cerevisiae ...
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bioRxiv - Bioengineering 2024Quote: Up to 1 × 108 yeast cells from a growing culture were taken to perform a zymoprep using the ZymoprepII kit (D2004, Zymo Research). The TRACeR sequences were amplified from the extracted plasmids and the adapter sequences were added by PCR ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from yeast cells grown into SD-Trp-Leu to mid-log phase was isolated with YeaStar RNA Kit (Zymo Research R1002). RNA was treated with 0.02 units/µl DNase I (NEB M0303 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Microbiology 2020Quote: ... cerevisiae were isolated using a yeast plasmid miniprep kit (Zymo Research) and transformed into High Efficiency NEB®5-alpha competent E ...
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bioRxiv - Microbiology 2022Quote: ... coli using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research).
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bioRxiv - Plant Biology 2024Quote: ... using the commercial kit Frozen-EZ Yeast Transformation IITM (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using Frozen-EZ Yeast Transformation II Kit (Zymo Research, California, USA). Transformants were selected on SD medium (Takara Bio ...
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bioRxiv - Cell Biology 2021Quote: ... was treated with Frozen-EZ Yeast Transformation II (Zymo Research, California, USA) to generate competent cells and was then transformed with bait and prey plasmids ...
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bioRxiv - Microbiology 2022Quote: ... and plasmid was isolated using 96-well yeast miniprep kits (Zymo D2005) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... cerevisiae strain EBY100 using Frozen-EZ yeast transformation Kit II (Zymo Research). Transformants from a single colony on Trp-deficient SDCAA plates were grown and used for subsequent yeast experiments ...
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bioRxiv - Immunology 2022Quote: ... Transformations were performed by using EZ yeast transformation kit (Zymo research, T2001). With EBY100 strain sand yeast cells were selected directly in selective SD-CAA media (6.7 g/l yeast nitrogen base ...
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bioRxiv - Immunology 2020Quote: ... cerevisiae (EBY100) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research) following the manufacturer’s protocol and recovered in selective SDCAA media.
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bioRxiv - Synthetic Biology 2023Quote: ... cerevisiae CEN.PK2-1D using frozen-EZ yeast transformation II kits (Zymo Research). The resulting yeast strains were cultivated at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... we used the Frozen-EZ Yeast Transformation II kit (Zymo Research #T2001) with slight modifications ...