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Citations for Zymo Research :
2051 - 2100 of 2450 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in TRIzol buffer and RNA was isolated using RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Immediately following digestion the reaction was stopped in the binding buffer from a Zymo ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205) and purified following the protocol in the kit ...
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bioRxiv - Genomics 2019Quote: ... and RRBS Libraries were prepared from 40 ng of genomic DNA digested with Msp I and then extracted with DNA Clean & Concentrator™-5 kit (Zymo Research). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines (www.illumina.com) ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mixture was cleaned and concentrated to 10 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). 1 μl of the codon randomised mRFP library was transformed into electrocompetent DH10B cells (20 μl competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using TRIzol-based (Fisher: 15596026) phenol-chloroform extraction and purified with RNA Clean and Concentrator-5 spin columns (Zymo research: R2050). RNA concentration and quality was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Systems Biology 2019Quote: ... for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research, D4003T). Libraries were resolved on a 2% MetaPhor agarose (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Cross-links between the DNA fragments and histones were reversed and the DNA fragments were recovered using the ChIP DNA Clean & Concentrator Kit (Zymo, Irvine, CA). Two biological replicates were performed for each group ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 10 min at 37°C before quenching using a Zymo Genomic DNA Clean & Concentrator 10 (Zymo Research D4011) kit according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-S-UTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research, R1013) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were fragmented to around 300nt length at 95°C for 1min and purified using RNA clean and concentrator (Zymo research, R1016). The fragmented RNA was then subjected to a 5’ dephosphorylation reaction at 37°C for 30min by adding rSAP (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Plant Biology 2023Quote: ... The RNA was then isolated from the membrane using phenol-chloroform and concentrated with the RNA Clean and Concentrator kit (Zymo Research, R1013), followed by reverse transcription using the RT Oligo primer (Supplementary Data 12 and SuperScript III ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Microbiology 2023Quote: ... The MDA-amplified VLP DNA was then processed using the Zymo Genomic DNA Clean and Concentrator-10 kit (Zymo Research, CA, U.S.A) and eluted in Zymo DNA Elution Buffer ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by end-repair using T4 PNK after purification of the rRNA depleted samples using zymo oligo clean and concentrator kit (Zymo research, # D4060). The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA from the heat lysed ZeptoMetrix MTB verification panel samples was purified and eluted in 40μL TE using Oligo Clean & Concentrator (Cat# D4061, Zymo Research, CA, USA). 5μL of extracted DNA was used as input for the LAMP assay.
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were placed on a magnetic stand and DNA-containing supernatant was cleaned using ChIP DNA Clean & Concentrator (Zymo Research, D5201). Transposed libraries were amplified in 50 μl PCRs (25 µl NEBNext HiFi 2× PCR master mix ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
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bioRxiv - Biophysics 2021Quote: ... the amplified backbone and insert DNA were cleaned up by digestion of template DNA using the restriction endonuclease DpnI and purified with a microcolumn-based system (Zymo Research, USA). The plasmid construct was transformed into an electrocompetent strain of Escherichia coli DH10β cells (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The aqueous phase was transferred to another tube by pipette and cleaned up using a Zymo cleanup and concentrator kit (Zymo Research D4013), then subject to size selection using SPRI-select beads (Beckman Coulter B23317 ...
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bioRxiv - Molecular Biology 2021Quote: ... one each with the insert in genomic and in antigenomic orientation for plasmid stock preparation using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... The presence of inserted sequences in generated vaccine virus stocks was confirmed by RNA extraction (Direct-zol RNA kit, Zymo Research) followed by RT-PCR (qScript XLT ...
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bioRxiv - Molecular Biology 2019Quote: ... Genomic DNA was isolated from 1 ml quiescent fractions (YeaStar kit, Zymo Research, Protocol I), and the DNA eluted with 60 μl of TE buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was conducted using a CTAB protocol combined with the Zymo DNA clean and concentrator kit (Zymo Research Corp., Orange, CA, USA) as reported previously [71] ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA sample was then extracted with chloroform and the water layer was further purified using DNA Clean & Concentrator kit (Cat. D4029, Zymo Research, California, U.S.). The samples were then sent for next generation sequencing using the BGI-Seq platform (BGI ...
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bioRxiv - Genomics 2022Quote: ... 10 μl of ligated product was added to 40 μl nuclease-free water before performing the column purification with RNA Clean & Concentrator™ (Zymo Research, R1016). After that ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...