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Citations for Zymo Research :
2001 - 2050 of 2450 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Elutions were subsequently de-crosslinked overnight at 65 °C and DNA was purified using ChIP DNA Clean and Concentrator (Zymo Research) and DNA was used for qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA was isolated and DNase treated the same way as for sequencing and cleaned with RNA Clean & Concentrator (Zymo Research). cDNA was synthesized with oligo-dT primer (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: 10 μl aliquots were removed at given time points and desalted using a ZYMO Oligo Clean & Concentrator spin column (ZYMO Research). The isolated material was resuspended in 30 μl 100 mM Na+-HEPES (pH 7.50 ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Cell Biology 2020Quote: ... pombe genomic DNA was isolated using a Quick-DNA Fungal/Bacterial Miniprep kit and purified by a Genomic DNA Clean & Concentrator-25 kit (both from Zymo Research). Genomic DNA was sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Libraries were size selected using TBE gels for 300-500 bp and DNA eluted using gel diffusion buffer and purified using ChIP DNA Clean & Concentrator (Zymo Research). Samples were quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch, R1012).
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Biochemistry 2022Quote: ... RNA isolation from the lysates was performed according to the Ambion/Life Technologies protocol using Trizol reagent and RNA Clean and Concentrator kit (Zymo Research). Isolated RNA pool was hybridized with TagSet master mix (nCounter Elements™ XT Reagents ...
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were then size selected to remove adaptor-dimers below 200 bp using three subsequent size-selection rounds with a Select-a-Size DNA Clean & Concentrator Kit (D4080, Zymo Research). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... The rRNA-depleted RNA samples were purified using the Zymo RNA Clean & Concentrator kit combined with DNase treatment on a solid support (Zymo Research). cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The reaction was incubated at 32°C for 4 hours before being treated with DNase for 20 minutes at 37°C then purified using an RNA clean and concentrate kit (Zymo Research). The RNA quality was checked using a MOPS/formaldehyde agarose gel electrophoresis.
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bioRxiv - Molecular Biology 2023Quote: ... The DNA pellet was resuspended in 20 µL 10 mM Tris pH 8 and further cleanup was done with a Zymo DNA Clean and Concentrator kit (Zymo Research).
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bioRxiv - Molecular Biology 2023Quote: ... The DNA from the input control and IP samples was isolated using the ChIP DNA Clean and Concentrator Kit (#D5205, Zymo Research) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA template for probe synthesis was generated by PCR amplification using T7 primers and purified using a DNA clean and concentrator kit (Zymo research). Antisense RNA probes were synthesized by in vitro transcription with either DIG-11-UTP (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Initial genomic library distributions were assessed on a 1.5% agarose gel and purified using a ZR-96 Genomic DNA Clean & Concentrator-5 Kit (Zymo Research, D4067). Library concentrations were quantified using the Quant-iT™ dsDNA Broad Range Assay Kit (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DpnI-treated inverse PCR vector and PCR product inserts were column purified using a DNA Clean and Concentrate Kit (Zymo Research). Inserts were added with 2-fold molar excess to 75 ng of the vector ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neocortical regions of slice cultures using Trizol reagent followed by RNA Clean & Concentrator kit (Zymo research R1014). cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891 ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and mixed with 450 μl ethanol and transferred to an RNA Clean & Concentrator-5 column (Zymo Research) for downstream RNA purification and concentration ...
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bioRxiv - Neuroscience 2023Quote: ... 88∼135 bp) were resolved on a 10% TBE polyacrylamide gel and extracted separately using the DNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37°C and further purified by using a Zymo DNA Clean and Concentrator column (Zymo Research, D4033).
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Microbiology 2023Quote: RNA was purified and concentrated according to the protocol for RNA Clean and Concentrator Kit - 5 (Zymo Research Corporation, Irvine, CA) with an eluted volume between 30-35µL ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified by phenol-chloroform extraction and the aqueous fraction was further cleaned using DNA Clean & Concentrator-5 with Zymo-Spin Columns (Zymo Research). Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA was isolated from Transwell filters (multiple filters were pooled per timepoint) and concentrated over RNA Clean & Concentrate (RCC) columns (Zymo Research). RNA quality was assessed by Agilent Bioanalyzer and all samples had a RIN > 6.3 ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Molecular Biology 2023Quote: ... the non-poly(A) RNA segments in the flow-through were purified using the RNA Clean & Concentrator-5 kit (Zymo Research). The resulting purified poly(A ...
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bioRxiv - Bioengineering 2023Quote: ... and RFs with a size greater than 17nt was isolated according to the RNA Clean and Concentrator-25 kit (Zymo Research). After obtaining ribosome footprints ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Transposition reactions were incubated for 30 min at 37°C while shaking at 1,000 rpm and cleaned using DNA Clean & Concentrator-5 (Zymo Research, D4003). Transposed libraries were amplified in 50 μl PCRs (0.5 μl Q5 DNA polymerase ...
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bioRxiv - Immunology 2024Quote: ... tubes were centrifuged at 12,000 × g for 15 min at 4°C and aqueous phase were transferred into a new tube and RNA was extracted with the RNA Clean and Concentrator kit (ZYMO Research).
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bioRxiv - Biophysics 2024Quote: ... or downstream of the poly(A) segment (Trichoplax AKDF19383) and purified via ethanol precipitation or DNA Clean & Concentrator-5 columns (Zymo Research). mRNA was transcribed for 2-2.5 h with mMESSAGE mMACHINE kits (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Products were loaded on a 2 % agarose gel and the appropriate bands were excised and cleaned up with the Zymoclean Gel DNA Recovery Kit (Zymo Research). All purified amplicons were shipped to Macrogen Europe B.V ...
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bioRxiv - Microbiology 2023Quote: ... Samples were immediately stored in 1 ml of DNA/RNA Shield presentation buffer (Zymo) and frozen at -20°C until DNA extraction.
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bioRxiv - Genetics 2020Quote: ... viral RNA was prepared from 200 µl of clarified virus stocks (3.000 rpm, 5 min) with the Quick-RNA Viral Kit (Zymo research) and eluted in 14 µl RNase free H20 ...
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bioRxiv - Plant Biology 2020Quote: ... and then both ribosome-bound RNA and denatured total RNA samples were purified with RNA Clean & concentrator-25 kit (Zymo Research, USA). RPFs were size-fractionated on 15% urea polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...