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Citations for Zymo Research :
151 - 200 of 208 citations for Prestained SDS PAGE Standards Single Color since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... High molecular weight DNA extracted from whole blood by standard protocols was bisulfite converted with the EZ-96 DNA Methylation-Gold Kit (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... from weight-normalized contents from the distal small intestine and proximal colon after addition of Low Abundance Microbiota Standard (Zymo) and extraction using the Zymo DNA Mini kit (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... 0.5% [w/v] SDS) and transferred into a chilled tube containing zirconia beads (ZYMO Research, Irvine, CA, USA). Primary extractions of RNA were performed with acidic phenol:chloroform (5:1 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of 10% SDS was added and purified through RNA Clean & Concentrator-25 Kit (R1017, Zymo Research) (following protocol for > 200 nt RNA) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 fully grown GV oocytes (an equivalent of 40 haploid genomes and 80 single-stranded DNAs after bisulfite conversion) were directly subjected to EZ DNA Methylation-Direct kit (Zymo Research) for bisulfite conversion with the following modifications ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Molecular Biology 2020Quote: ... The intermediate RNA products were removed with NaOH-EDTA solution in 95 °C for 10 minutes and the single strand DNA oligo probes were purified via DNA clean & concentrator-25 (D4033, Zymo Research). The ssDNA probes were either directly used for Oligopaint FISH or store in aliquots at −80°C.
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bioRxiv - Plant Biology 2023Quote: ... microdissected material of around 80 gynoecia (sections of around 24 paraffin blocks with 3 or 4 embedded inflorescences) was pooled in a single tube and RNA extraction was performed with Direct-zol RNA MicroPrep kit (Zymo Research). The integrity and concentration of extracted RNA of each sample was measured using a NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA). Small RNAs were prepared by GenXPro (GenXPro GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA).
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bioRxiv - Genetics 2019Quote: ... Small RNAs were sized selected and purified from a 15% denaturing polyacrylamide-urea gel using the ZR small-RNA™ PAGE Recovery Kit (Zymo Research). For oxidized RNA library preparations ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction reagent only controls were included for each batch of DNA extractions along with ZymoBIOMICS Microbial Community Standards (Zymo Research) and E ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 2.5 μl 10% SDS and purified using the DNA Clean & Concentrator Kit (Zymo). 20 μl of the transposed DNA was then amplified using the NEBNext High-Fidelity 2x PCR Master mix with i5 and i7 Nextera barcoded primers for 12 cycles (see Supplementary Table 6 for primer sequences) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated from tumor cell populations and unstained whole tumor single cell suspensions using the Quick-RNA™ Microprep Kit (Zymo R1050) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Single-fly genomic DNA preps were prepared using the solid tissue protocol of the Quick-DNA™ Miniprep Plus Kit (Zymo Research). In total ...
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bioRxiv - Genomics 2022Quote: ... separating 22 or 10 (control wells) single nuclei per well in a 96-well plate containing 2.5 μL of M-digestion buffer (Zymo, Cat. D5020-9), 0.25 μL of Proteinase K (Zymo ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Microbiology 2019Quote: ... and a mock microbial community DNA (8 bacterial + 2 yeasts species mixed in defined proportions) (ZymoBIOMICS Microbial Community DNA standard, Zymo Research, USA) were included in the experiments ...
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bioRxiv - Plant Biology 2019Quote: ... to several others we added a mock microbial community as a positive control (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We extracted DNA using the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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bioRxiv - Genomics 2023Quote: Reads were aligned to the 16S genes extracted from the complete reference genomes of the bacteria present in the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) using minimap2 ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 10 trunk fragments per biological replicate using the standard Trizol RNA extraction protocol with high salt precipitation and clean-up on Zymo RNA columns (Zymo R1013/R1017). RNA (1 μg ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... The ribosome pellet was resuspended in a solubilization buffer containing 0.5% SDS and 1mM EDTA and TRIzol before RNA was extracted using the Direct-zol kit (Zymo Research). RNA was separated on a 15% TBE-Urea gel before RNA between 17nt and 34nt were gel extracted ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted via-SDS lysis and phenol-chloroform extraction and further purified using Clean and Concentrator columns (Zymo Research). DNA concentration was determined using a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
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bioRxiv - Microbiology 2022Quote: ... pisum LSR1 RNA was extracted from a single aphid using the Zymo Quick RNA Miniprep Kit according to the manufacturer protocols (Zymo Research, CA, USA). The RNA was then reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... buffer without any added fecal material) and two microbial mock communities as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, California, USA).
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... we also produced ten separate V3-V4 16S rRNA gene amplicon libraries from the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA) using the 2-Step library preparation method ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was extracted from the iPSC colonies after single cell sorting and clonal expansion by using the Quick-DNA™ Miniprep Kit (Zymo Research 11-317A) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Two microdissected punches (left and right hemisphere) were taken from a single brain slice and stored in DNA/RNA Shield (Zymo Research, Irvine, CA, USA) at −80∘C until further processing ...
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bioRxiv - Immunology 2021Quote: ... Single viable CFSElow CD3+ CD8− CD4+ T cells were sorted into 96-well PCR plates containing DNA/RNA Shield (Zymo Research, Irvine, CA, USA) using FACSAria II and Diva Software (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer specificity was tested by performing ddPCR in the presence of an excess of eukaryotic (fish) or prokaryotic [ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA)] DNA ...