Labshake search
Citations for Zymo Research :
101 - 150 of 6356 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Biochemistry 2019Quote: ... the DNA samples used for 450k analysis were digested overnight at 37°C using BamHI and EcoRI and then bisulfite converted using EZ DNA Methylation-Lightning Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
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bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The reaction was incubated at 32°C for 4 hours before being treated with DNase for 20 minutes at 37°C then purified using an RNA clean and concentrate kit (Zymo Research). The RNA quality was checked using a MOPS/formaldehyde agarose gel electrophoresis.
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bioRxiv - Immunology 2023Quote: ... incubated for 15 min at room temperature and either frozen at -80°C or directly followed by RNA isolation using a Direct-zol RNA microprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Two hundred and fifty nanograms of linker-ligated genomic DNA were subjected to sodium bisulfite conversion for 210 min at 64°C using the EZ DNA Methylation Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research) before transfection (Supplemental Fig ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was prepared from fibroblasts isolated from mouse hearts using Direct-zol RNA miniprep kit (Zymo Research). cDNA libraries were prepared using the NEBNext Ultra Directional Library Preparation Kit following rRNA depletion using the NEB rRNA depletion kit ...
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bioRxiv - Molecular Biology 2024Quote: Mouse back skin was homogenized and RNA was extracted using Quick-RNA Miniprep Kit (Zymo Research, Cat# R1054) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... was used for bacterial 16S ribosomal RNA targeted sequencing and custom 16S primers were utilized to amplify the V3-V4 region (Zymo Research, Irvine, CA). Real-time PCR was then used to prepare the sequencing library and final qPCR fluorescence readings were pooled together according to equal molarity and the final pooled library was cleaned using the Select-a-Size DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Systems Biology 2019Quote: ... for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research, D4003T). Libraries were resolved on a 2% MetaPhor agarose (Lonza ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Immunology 2021Quote: Tissue samples were collected and stored in RNAlater solution as described above and prepared for RNA isolation as follows: Isolated brain regions were homogenized in lysis buffer using BashingBeads Lysis tubes (Zymo Research Europe, Freiburg, Germany) and isolated using AllPrep DNA/RNA Mini Kit (Qiagen ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Punches from each of these brain regions were placed in individual tubes containing 2□mm size beads (ZR BashingBeads Lysis Tubes, Zymo Research, item S6003-50) and 1 mL of QIAzol lysis reagent (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from mouse iBAT following the standard protocol for the Direct-zol RNA Miniprep kit (Zymo Research) and assessed for quality on the Agilent 2200 TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was extracted from mouse kidneys and livers by the Direct-zol™ RNA Purification Kits (Zymo Research). Purified RNA was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA from archived (stored at -80°C) American alligator whole blood was isolated using the Quick-RNA Whole Blood Kit (Zymo Research, Irvine, CA). Samples from both LW and CFR were selected randomly based only on the availability of samples ...
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bioRxiv - Microbiology 2021Quote: ... Each strain was grown in 1 ml of LB+CA100 at 30°C for 48 hours and genomic DNA was extracted using a Quick-DNA High Molecular Weight kit (Zymo Research, cat#D6060) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... chromatin was de-crosslinked for 15 minutes at 65°C and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research, Freiburg, Germany). Binding of Msh2 ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was frozen at -20°C until DNA extraction using a Zymo fecal/soil DNA extraction kit according to enclosed directions (Zymo Research, Irvine, CA). After sonication ...
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bioRxiv - Microbiology 2020Quote: ... isolates were grown shaking in SG broth at 37°C and genomic DNA isolated using the Quick-DNA Fungal/Bacterial miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's suggested protocol.
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bioRxiv - Developmental Biology 2020Quote: DNA was isolated from cells using the Quick-gDNA miniprep kit (Zymo). DNA (50ng ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were processed using the YeaStar RNA Kit (Zymo Research, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: DNA was isolated from cells using the Quick-gDNA miniprep kit (Zymo). DNA (50ng ...
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bioRxiv - Cell Biology 2021Quote: DNA was collected from sorted subsets of cells Quick DNA kit (Zymo). Lentiviral insertions in genomic DNA were amplified by nested PCR ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from cells using commercial RNA extraction kits (Zymo Research). RNA (1ug ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was harvested from cells with the Direct-zol miniprep kit (Zymo). Harvested RNA was prepared for sequencing with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from cells using commercial RNA extraction kits (Zymo Research). RNA (1ug ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from cells using Quick-RNA kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... glabrata cells were prepared using Frozen-EZ Yeast Transformation Kit (Zymo Research) and transformation followed an electroporation-based protocol described previously (49 ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was extracted from cells with Direct-zol-96 RNA Kit or Direct-zol RNA MiniPrep Kit (Zymo Research). RNA samples were subjected to qRT-PCR analysis using AgPath-ID One-Step RT-PCR Kit (Applied Biosystems ...