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Citations for Zymo Research :
1301 - 1350 of 1353 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Microbiology 2023Quote: ... Remaining material from the same DNA extract of subculture 19.9 was then concentrated using the DNA Clean and Concentrator-5 kit (Zymo Research; Irvine, California, U.S.A.) and used for long read sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was removed from the purified RNA samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014). For rRNA-depleted samples ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified and the DNA oligos were removed from samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e. 1X PBS, pH 7.2 or Zymo DNA/RNA shield). Swabs in PBS were placed on ice and sent for culturing while the other swabs were transported back at room temperature to the laboratory and stored at -80°C ...
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bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Products were loaded on a 2 % agarose gel and the appropriate bands were excised and cleaned up with the Zymoclean Gel DNA Recovery Kit (Zymo Research). All purified amplicons were shipped to Macrogen Europe B.V ...
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bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Isolates for sequencing were cherrypicked using an Opentrons OT-2 Liquid Handler and DNA extraction was then performed using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) on a KingFisher Flex instrument (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... Pools 4-8 from experimental replicate #2 were resuspended in 100 µL spheroplast digest buffer with 2.5U/mL Zymolyase T100 (Zymo Research #E1005) and incubated at 37°C for 20 minutes ...
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bioRxiv - Genomics 2024Quote: ... for 2 hr at 37 C and then cleaned and concentrated using a Zymo DNA Clean and Concentrator column (Zymo D4013). The debranched RCA product was run on a 1% low-melt agarose gel ...
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA extractions were performed upon 5 million trophozoites of Nf69 using Quick-DNA Miniprep Plus Kit according to manufacturer recommendations (Zymo Research, Irvine, CA, USA). In short ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Microbiology 2023Quote: ... a 4.5 ml sample of each microcosm and extracted bacterial DNA with a Quick-DNA Fecal/Soil Microbe Miniprep Kits (Zymo Research, Irvine, California, USA). Two negative controls that contained the SH salt medium alone were included in the process of DNA extraction ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Neuroscience 2023Quote: RRBS libraries were prepared from 200 ng of genomic DNA digested with MspI restriction enzyme and then extracted with ZR-DNA Clean & Concentrator™-5 kit (Zymo Research, Cat# D4014). According to Illumina’s specified guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
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bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl of 0.5 M EDTA was added to this mixture and RNA fragments were column purified (Zymo research Cat no-D4061) and eluted in RNAse free water ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a series of 8 serial dilutions of a known mock microbial community consisting of 8 gram positive and negative bacteria and 2 yeast (Zymo Research, catalog # D6300), as previously described (31) ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
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bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
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bioRxiv - Zoology 2023Quote: ... for 3 min at 28 Hz in 2 mL microcentrifuge tubes in 1X sterile PBS or four volumes of DNA/RNA Shield (Zymo Research, Irvine, CA) and two 5 mm stainless steel grinding balls ...
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bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... we first made a PCR standard by gel extracting the 93-bp PCR amplicon produced from the GAPDH primer/probe set using a 2% agarose gel and Zymo DNA Gel Extraction kit (Zymo cat. no. D4007). The concentration of gel-extracted GAPDH amplicon was quantified with a QuBit high sensitivity dsDNA assay and then diluted in nuclease-free water to create 10-fold serial dilutions ranging from 1e9 to 1e2 molecules/uL ...
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bioRxiv - Genomics 2024Quote: ... and 2 µL of DNA isolated using the Quick-DNA Fecal-Soil Microbe kit or the Quick-DNA Miniprep kit (Zymo Research, Irvine, CA). Reactions were run in a BioRad C1000 Touch (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Punches from each of these brain regions were placed in individual tubes containing 2□mm size beads (ZR BashingBeads Lysis Tubes, Zymo Research, item S6003-50) and 1 mL of QIAzol lysis reagent (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...