Labshake search
Citations for Zymo Research :
1151 - 1200 of 1353 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... For RNA analysis 5% of input material and 70% of elution were processed by the addition of 400 µl RNA lysis buffer (Zymo) and RNA extraction using the Zymo Quick-RNA™ MicroPrep RNA extraction kit (Zymo ...
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bioRxiv - Molecular Biology 2020Quote: ... The enriched RNA and input total RNA from 5% of lysate were purified using Direct-zol RNA Purification Kit (Zymo). Target genes in input total RNA and enriched RNA were quantified by qRT-PCR using SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... the transposed DNA fragments were harvested and cleaned up with Zymo DNA Clean and Concentrator-5 kit (D4011, Zymo Research). The libraries were indexed and amplified with Illumina i5/i7 indexing primers (FC-121-1011 ...
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... Lysis tubes were shaken 5 min at 16 Hz using a ball mill to extract and purify DNA following the manufacturer’s guidelines (Zymo Research). Microbiome profiling of isolated DNA samples was performed by Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembly is cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 3 μl Gel Loading Dye ...
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bioRxiv - Bioengineering 2022Quote: ... The IVT reaction was assembled based on the manufacturer’s recommendations and the sgRNA was purified using an RNA Clean & Concentrator-5 kit (Zymo Research). RNA concentration was measured using a NanoDrop (ThermoFisher)
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bioRxiv - Plant Biology 2021Quote: ... The RNA was then purified and concentrated using the RNA Clean Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). cDNA libraries were prepared using a mix of 50 ng RNA and 0.5 ng Spike-in RNA Variant Control Mix E2 (Lexogen ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated using the PerfectPure RNA Cultured Cell kit (5 PRIME) or Quick-RNA™ MiniPrep (Zymo Research). One microgram of the total RNA was reverse-transcribed into cDNA with M-MLV (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were purified from the reaction using a RNA Clean & Concentrator(tm)-5 column (Zymo Research, Cat. n° R1013). The resulting RNA fragments were ligated to different adaptors (listed in Supplementary Table 1 ...
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bioRxiv - Genetics 2020Quote: ... viral RNA was prepared from 200 µl of clarified virus stocks (3.000 rpm, 5 min) with the Quick-RNA Viral Kit (Zymo research) and eluted in 14 µl RNase free H20 ...
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bioRxiv - Genetics 2021Quote: RNA was isolated from eight 5 days old males using ZR Tissue and Insect RNA MicroPrep™ (Zymo Research, #R2030). DNA was removed using TURBO™ DNase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RNA was cleaned and concentrated to 10 μl using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA), as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... P7 and E18.5 was directly dissolved in RNA isolation buffer and RNA was isolated using the Quick-RNA micro prep kit (Zymo). Library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit ...
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from ∼6x105-7x105 MRC-5 infected fibroblasts using Zymo Duet RNA/DNA isolation Kit (Zymo Research). Viral genomes were quantitated by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification and plasmid isolation was done using the DNA Clean & Concentrator-5 and Zyppy Plasmid Miniprep Kits from Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted and purified using Trizol reagent and RNA Clean & Concentrator-5 kit (Zymo Research cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the aqueous phase was transferred to an RNA Clean and Concentrator-5 column and purified according to the manufacturer’s instructions including on-column DNaseI digestion (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Cell Biology 2022Quote: ... all wells were pooled to purify DNA products using the Zymo DNA Clean & Concentrator-5 kit (Zymo, Cat #: 11-303) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Microbiology 2024Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
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bioRxiv - Immunology 2024Quote: ... and the resulting products were purified using the DNA Clean and Concentrator-5 Kit following the manufacturer’s instructions (Zymo Research). In-Fusion reactions were prepared in 5 µL volumes at a 1:3 vector to insert molar ratio ...
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bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Molecular Biology 2024Quote: ... a final round of PCR was performed by taking 100 ng of amplified sgRNA library and 5 cycles of the above PCR program was run followed by Zymo column clean up ...
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bioRxiv - Systems Biology 2020Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Plant Biology 2021Quote: ... 500 pg - 2 µg DNA was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research). Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1 ...
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bioRxiv - Genomics 2021Quote: ... RNA was isolated from 2 day-differentiated TXΔXicB6 cells using the Direct-Zol RNA Miniprep Kit (Zymo Research). To remove any remaining gDNA ...
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bioRxiv - Immunology 2021Quote: Total RNA isolations from SARS-CoV-2 were carried using DIRECT-ZOL RNA Miniprep Kits (Zymo Research, USA), and concentrations were determined using QUBIT fluorometer with the QUBIT RNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 2 x 500 ng of DNA was bisulfite modified with the EZ DNA Methylation-Lightning kit (\#D5030, Zymo) and standardized to a concentration of 25 ng/uL BC-DNA ...
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bioRxiv - Systems Biology 2023Quote: ... We then sequentially added and centrifuged through the column 400 µL DNA/RNA prep buffer (Zymo, #D7010-2), 600 µL DNA/RNA wash buffer (Zymo ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 2×106 NCI-H295R cells was isolated with the Quick-DNA Miniprep Kit (Zymo research). Bisulfite treatment was performed using the EZ DNA Methylathion™ Kit (Zymo Research) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 min at 30Hz and extracted RNA using the Direct-Zol RNA MiniPrep kit (Zymo Research, USA) with on-column DNA digest according to the manufacturer protocol ...
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bioRxiv - Genomics 2024Quote: The PCR reaction contained 2 µl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 µl of buffer + 0.05 µl of Taq Polymerase (PCRBIO Classic Taq ...
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bioRxiv - Microbiology 2023Quote: ... control samples (n = 4, one for each extraction batch) using commercially available kits optimized for fecal-based microbial communities (Zymo fecal/soil kit), and some aliquots archived ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the PMA-treated soil samples (n = 48) and the no-template (water) controls (n = 4) using the Quick-DNA Fecal/Soil Kit (Zymo Research, Freiburg, Germany), which is designed explicitly for soil-based microbial communities ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...