Labshake search
Citations for Zymo Research :
1251 - 1300 of 1551 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was also performed on three no-sample added (negative) and three ZymoBIOMICS Microbial Community Standard (positive) controls (Zymo Research, Irvine, CA, USA). DNA from an additional eight GWSS had been previously extracted using the Blood and Tissue kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: The soil microbiome DNA was extracted using 200 mg of soil from each pot and the Zymo Quick-DNA™ Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA). DNA purity was assessed using spectrophotometry (NanoDrop model ...
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bioRxiv - Microbiology 2023Quote: vIL-6/THP-1 and parental THP-1 cells were infected with or without r219.KSHV for 72h and RNA was purified using the Quick-RNA miniprep kit (Zymo Research, Irvine, CA, USA). Indexed ...
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bioRxiv - Neuroscience 2023Quote: DNA from each pool of 5-HT+ or 5-HT-cells was isolated and bisulfite-converted using the EZ DNA Methylation-Direct Kit (Zymo Research, Irvine, CA, USA) with the following adaptations ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole placentas were equilaterally quartered and homogenized in RNA Lysis buffer and RNA was extracted using the Zymo QuickRNA miniprep kit (Zymo Research, Irvine, CA USA) according to manufacturers recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Pith samples had low concentrations of DNA and were subsequently cleaned and concentrated into 35 µl using the Zymo Clean & Concentrate-25 kit (Zymo Research Corporation, Ca, USA). For all samples ...
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bioRxiv - Genomics 2023Quote: ... Three DNA libraries from the same DNA extraction were prepared with further purification and concentration using the Genomic DNA Clean and Concentrator kit (Zymo Research, Irvine, CA, USA). The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio ...
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bioRxiv - Microbiology 2023Quote: ... Large-scale DNA plasmid was amplified in Escherichia coli DH5α and purified by using a plasmid giga prep kit (Zymo Research, Costa Mesa, CA). DNA sequencing was performed to confirm the plasmid sequence’s authenticity.
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bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from the third eyelid of dromedary C3 with Zymo® according to the manufacturer’s instructions (Zymo Research, Irvine, CA, USA). RNA was quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Neuroscience 2024Quote: ... The aqueous layer of the phase separation was applied to the column of the Direct-Zol RNA Mini Kit (Zymo; Irvine, CA; Cat #: ZR2070), and isolated following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Collections from multiple donors were pooled and homogenized in one large suspension together with the microbiome preservative reagent DNA/RNA Shield™ (Zymo Research – CA, USA) to prevent outgrowths or depletions of microbial taxa ...
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bioRxiv - Plant Biology 2024Quote: The DNA was extracted from 250 mg of soil using the Zymo Quick-DNA™ Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA). DNA purity was assessed using spectrophotometry (NanoDrop model ...
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bioRxiv - Genomics 2024Quote: ... DNA and RNA were extracted from wastewater samples were extracted using the Zymo Environ Water RNA Kit (Cat No. R2042, Zymo Research Corp, Irvine, CA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA was isolated from the cyclin A/B RxL inhibitor (CIRc-004) and enantiomer control (CIRc-005) treated arms using Quick-RNA™ Miniprep kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 100 mg of frozen leaf tissue per sample using either Direct-zol® RNA Purification Kit or Quick-RNATM Miniprep Kit (Zymo Research, CA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted directly from the TRI reagent using a Direct-Zol RNA Mini-Prep Kit according to manufacturer instructions (Zymo Research Corporation, Irvine, CA). RNA from all four groups was processed using a Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) ...
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bioRxiv - Microbiology 2024Quote: ... fecal samples with the ZymoBIOMICSTM DNA Miniprep Kit and the Quick-DNATM Fecal/Soil Microbe DNA MiniPrep Kit (cat. D4300 & D6010, respectively; Zymo Research, Irvine, CA, USA), in accordance with their respective protocols ...
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA). Purified yeast-extracted DNA plasmid was then used as template to generate amplicons through two consecutive PCR reactions ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA plasmid present within the yeast cells was extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Purified yeast-extracted DNA plasmid was then used to transform competent E ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA constructs encoding single macrocyclic peptide ligand were verified by Sanger sequencing and further used to transform new competent EBY100 cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA, USA).
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Amplicons for next generation sequencing (NGS ...
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bioRxiv - Genomics 2024Quote: ... and three susceptible (n= 3) untreated seedlings were used for genomic high molecular weight DNA isolation using the Quick DNA HMW MagBead Kit (Zymo Research, Irvine, CA, USA). DNA integrity ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 volumes of adjusted RNA binding buffer (1:1 Zymo RNA Binding Buffer and 100% ethanol) is added to total RNA and vortexed ...
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bioRxiv - Neuroscience 2021Quote: ... or TRI Reagent (Zymo, #R2050-1) for gene expression analysis ...
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bioRxiv - Genomics 2021Quote: ... digestion with 1% zymolyase (Zymo Research) to weaken ascus walls ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: The PCR products were checked on an agarose gel stained with ethidium bromide before gel extraction (Zymoclean™ Gel DNA Recovery Kit, Zymo Research, Irvine, CA, USA). The resulting pure amplicons were ligated into pCR™-Blunt II-TOPO® (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... in the vagina with the other hand and rotate it for 10-15 seconds before placing the swab into the provided collection tube (FluidX tubes; 65-7534 - Brooks Life Sciences, Chelmsford, MA, USA; containing 0.8 ml DNA/RNA-shield; R1100-250, Zymo Research, Irvine, CA, USA) and breaking off the handle ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then transferred to a new tube and further processed on columns using the Direct-zol™ Kit (Zymo Research, Irvine, CA, USA). RNA was treated in-column with DNAse and eluted in nuclease-free water before being stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research, Irvine, CA, USA), as previously described (Kongprajug et al. ...
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bioRxiv - Microbiology 2022Quote: ... individual ticks were transferred to separate wells of a 96 well deepwell plate that was pre-loaded with a single 5mm steel ball bearing (OMNI International, GA, USA) and 400uL of 1X DNA/RNA shield (Zymo Research Corp., Irvine CA, USA) in each well ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... and using sterile forceps placed in ZR-96 BashingBead™ lysis tubes containing 750 μl DNA/RNA Shield™ (Zymo Research Corp., Irvine, CA, USA). The samples were shaken and placed on ice until they returned to the vehicle ...
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bioRxiv - Genomics 2022Quote: ... total RNA was extracted from peripheral white blood cells using the Zymo Research Direct-zol™ DNA/RNA Miniprep kit (Zymo Research Corporation, Irvine, CA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... A total of 500 ng of genomic DNA from cells in co-cultures were bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA). The Infinium HD Methylation Assay (bisulfite modification ...
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bioRxiv - Microbiology 2023Quote: DNA extraction was performed from 4g of frozen bedding samples (-80°) or from teat skin swabs with the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The bisulfite conversion of sonicated genomic DNA fragments was carried out based on the instructions provided in the EZ DNA-methylation lightning Kit (Zymo Research Corp., Irvine, CA, USA). The libraries were made using the Accel-NGS Methyl-Seq DNA library kit (SWIFT Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The parasites were washed twice with PBS prior to RNA extraction and purification using the Quick-RNATM MiniPrep kit (Zymo Research, Irvine, CA, United States) according to manufacturer specifications ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Microbiology 2020Quote: An aliquot of each fraction was then mixed 1:1 with 2× DNA/RNA Shield (Zymo Research) spiked with in vitro transcribed RNA (generated by T7 transcription off a Firefly luciferase (Fluc)-containing plasmid) ...
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bioRxiv - Microbiology 2021Quote: ... Viral pellets were then resuspended in 200 μl PBS and viral RNA was extracted using Quick-RNA Viral Kit (Zymo Research, CA. Cat. No. R1034 & R1035) following the manufacturer’s protocol and eluted in 10 μl RNase-free water ...
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bioRxiv - Zoology 2021Quote: ... The supernatant was collected and added to 700 μL of 95% ethanol for total RNA recovery using the Direct-zol™ RNA mini-prep kit (Zymo Research®, Irvine, CA, USA). The solution was transferred to filter columns and centrifuged (16,000 g × 30 s) ...
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bioRxiv - Microbiology 2022Quote: ... a negative control to account for microbial contaminants present in DNA extraction kits or introduced during the extraction (Eisenhofer et al., 2019) and a microbial community standard (Zymo Research, cat. no. D6300, Irving, CA). The DNA concentration was quantified with Qubit 3 using the high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... Three sections from each sample were individually placed into Zymo Quick-DNA Fecal/Soil Microbe ZR BashingBead™ Lysis Tubes filled with DNA/RNA Shield™ (Zymo Research Co, Irvine, CA, United States). Homogenization and physical lysis of biomass samples was achieved in the field using a custom tube holder attached to a reciprocating saw ...