Labshake search
Citations for Zymo Research :
1151 - 1200 of 1551 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Up to 75 ng of sheared gDNA was subjected to bisulfite conversion using the EZ-96 DNA Methylation Kit (Zymo Research; Irvine, CA), with liquid handling on a Hamilton MicroLab STAR (Hamilton ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA (500□ng) from whole blood was bisulfite treated using the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4□μl of bisulfite- converted DNA was measured on the Illumina 450k array[14] following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and an aliquot of 500□ng of genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation Kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The Infinium Methylation EPIC BeadChip technology (Illumina ...
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bioRxiv - Genomics 2022Quote: ... 750 ng of DNA was extracted from whole blood and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 160 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50-100 mg seedlings using the Quick-RNA Miniprep Kit with on-column DNase I digestion (Zymo Research, Irvine, CA). cDNA was synthesized with 2–2.5 μg total RNA using the Invitrogen SuperScript III Reverse Transcriptase and the Oligo(dT)20 primer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... was cleaned and concentrated with either sparQ PureMag beads (QuantaBio, Beverly, MA) or the Genomic DNA Clean & Concentrator-10 Kit (Zymo Research, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Microbiology 2022Quote: ... samples were processed for RNA extraction and purification using the Zymo Direct-zol™ RNA Mini Prep kit (Zymo Research, Irvine, CA, USA) per manufacturer instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminants before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). Four to eight tubules were collected for each sample for a total length of 2 – 4 mm.
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bioRxiv - Plant Biology 2022Quote: ... The sheared DNA was cleaned up and recovered by 1.2x AMPure XP beads followed one round of sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research Corporation, Irvine, CA) as outlined in the manufacturer’s instruction with 80 min of conversion time ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acid was isolated with either the Quick Microprep Plus kit (Catalog D7005) or the Zymo Quick Miniprep Plus kit (Catalog D7003) (Zymo Research, Irvine, CA). Gel electrophoresis was performed as described above ...
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bioRxiv - Physiology 2022Quote: ... Samples thawed and then disrupted with an eppendorph tube pestle and total RNA purified using a Direct-zol plus RNA miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: HepG2 cell samples were collected into QIAzol Lysis Reagent and total RNA was extracted using Direct-zol RNA Miniprep (Zymo Research, CA, USA). Liver samples were first homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Bioengineering 2024Quote: ... Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research, Irvine, CA). The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity ...
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bioRxiv - Neuroscience 2024Quote: ... Larval and adult samples were completely homogenized in RNA Lysis buffer from the Quick-RNA miniprep Kit (R1055, Zymo Research, Irvine, CA, USA) using a pestle and micro-tube homogenizer (for 30 sec ...
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bioRxiv - Genetics 2024Quote: ... 2016) Genomic DNA (500ng) from whole blood underwent bisulfite treatment with the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4 µl of bisulfite-converted DNA was measured on the Illumina 450k array following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-500 ng of DNA from each sample was bisulfite-converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol and the specified modifications for the Illumina Infinium methylation assays ...
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bioRxiv - Genetics 2024Quote: ... Five hundred nanograms of DNA per sample were bisulfite-treated using the Zymo EZ-96 DNA methylation kit (Zymo Research, Orange, CA, USA). A bisulfite sequencing PCR approach was applied to the promoters of 12 candidate genes (Supplementary Table S3) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellets were washed with PBS before total RNA was extracted with the Direct-zol RNA Micro-Prep method (Cat. R2063-A, Zymo Research Corp., CA), and the extracted RNA was reverse transcribed using LunaScript® RT SuperMix Kit (Cat ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid and fosmid DNA was purified with either the Zyppy Plasmid Miniprep kit or ZymoPURE II Plasmid Midiprep kit (Zymo Research, Irvine, CA). Following restriction digests or PCR ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μL of each reaction product were pooled and purified using the Zymo DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing was performed at the Chan Zuckerberg BioHub facility on an Illumina NextSeq 550 platform in High Output mode.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was isolated from polarized murine bone marrow-derived macrophages using the Quick-RNA™ MiniPrep Kit (Zymo Research, Irvine, CA, USA). RNA quantity and quality was verified by two methods ...
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bioRxiv - Zoology 2023Quote: ... using the manufacturer’s instructions or co-extraction of DNA and RNA was accomplished with a Quick DNA/RNA MiniPrep Plus Kit (Zymo Research, Irvine, CA). The quality and quantity of DNA and RNA were analyzed by either NanoDrop 1000 or NanoDrop One (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... for 3 min at 28 Hz in 2 mL microcentrifuge tubes in 1X sterile PBS or four volumes of DNA/RNA Shield (Zymo Research, Irvine, CA) and two 5 mm stainless steel grinding balls ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA (500ll!ng) from whole blood was bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), following the standard protocol for Illumina 450K micro-arrays ...
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bioRxiv - Microbiology 2023Quote: ... patient wound slough was removed from the ulcer base with a dermal curette and immediately stored in RNA Shield (Zymo Research, Irvine, CA) at 4°C for 24 hours before being frozen at –80°C until further processing ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from approximately 3.0 × 105 EndoC-βH1 using Direct-zol RNA Miniprep Kit (cat. R202-Zymo Research, Irvine, CA, US) following manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... and urine samples underwent RNA extraction in the Virology Unit at the Institut Pasteur de Madagascar (IPM) using the Zymo Quick DNA/RNA Microprep Plus Kit (Zymo Research, Irvine, CA). In total ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was performed according to manufacturer’s protocol using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA, USA, #D6400). The pooled libraries were sequenced on an Illumina MiSeq using MiSeq®Reagent Kit v2 (500-cycle ...
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bioRxiv - Microbiology 2024Quote: Total genomic DNA was extracted from the vaginal samples using a modification of the ZymoResearch Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA) and FastPrep mechanical lysis (MPBio ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The liver of control and BPA lineage fish (9 females and 6 males) were used for total RNA extraction by using Quick RNA/DNA Miniprep Plus Kit (#D-7003, Zymo Research, CA, USA) according to the manufacturer’s protocol as previously described60 ...
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bioRxiv - Genomics 2024Quote: ... 750 ng of DNA was extracted from whole blood and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 160ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Genomics 2024Quote: ... 250 ng of DNA was extracted from muscle tissue and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 250 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 50 mg of soybean leaf tissue using the Direct-zol RNA Miniprep Plus Kit with DNaseI treatment (Zymo Research, Irvine, CA). RNA samples were quantified using a Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: HBV rcDNA and pgRNA were extracted from both mouse liver tissue and serum samples using a Quick-DNA/RNA Microprep Plus Kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 250 ng of DNA was extracted from adipose tissue and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 250 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... The pm-mCherry was then extracted and purified with the DNA Clean and Concentrator- 25 TM kit (D4033; Zymo Research, Irvine, CA, USA). The pm-mCherry was subsequently transcribed in vitro using the T7-FlashScribe kit (C-ASF3507 ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA of each muscle sample was extracted from approximately 50 mg of frozen tissue using Direct-zol RNA Miniprep - Zymo Research (Zymo Research, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... face, retroauricular crease (RAC), scalp, and toe web using swabs (Isohelix, Kent, UK) and preserved in DNA/RNA Shield (Zymo Research, Irvine, CA). Environmental samples were collected using swabs (hearth ash ...
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bioRxiv - Microbiology 2024Quote: ... Environmental samples were collected using swabs (hearth ash, soil, water) or Sterivex filters (water) and preserved in DNA/RNA Shield (Zymo Research, Irvine, CA). Sample collection was performed by a single individual following IRB-approved protocols (detailed in Supplemental Information).
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bioRxiv - Microbiology 2024Quote: ... Extraction of microbial DNA from the in situ samples was performed using a Zymo Quick-DNA Fecal/Soil microbe kit (Zymo Research, Irvine, CA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA/RNA hybrids were further purified following DNase digestion and additional purification using Zymo-SpinTM columns (ZYMO-RESEARCH CORP, Irvine, CA, USA). Extracts were treated with RNase (to remove all RNAs) ...
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bioRxiv - Microbiology 2024Quote: Libraries for mRNA sequencing were constructed from total RNA sample using the Zymo-Seq RiboFree Total RNA Library Prep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... two C57BL/6 wild-type murine cecal samples (generated previously in [18]) and one mock community (ZymoBIOMICS Gut Microbiome Standard, Zymo Research, Irvine, CA) were used to test the efficacy of the primer sets across multiple sample types ...