Labshake search
Citations for Zymo Research :
1201 - 1250 of 1620 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... DNA samples were plated randomly across 96-well plates and provided to the Emory University Integrated Genomics Core for bisulfite modification using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA), and subsequent assessment of genome-wide DNAm using the Illumina MethylationEPIC Beadarray (Illumina ...
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bioRxiv - Microbiology 2020Quote: The double stranded deoxyribonucleic acids (dsDNA) was extracted from the duplicate sludge samples using ZR soil microbe DNA kit (Zymo Research, CA., USA), according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted according to manufacturer specifications using the Zymo Quick-DNA Fungal/Bacterial Miniprep kit (Cat# D6005, Zymo Research Corp., Irvine, CA). For community characterization ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from bacterial suspensions prepared from tissue homogenates was extracted and purified using the Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA). All extracted DNA was stored in the supplied kit elution buffer at −20°C until polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2020Quote: The respiratory samples were prospectively stored at −80°C after stabilization by mixing an equal volume of DNA/RNA Shield (2X concentrate; Zymo Research, Irvine, CA). The thawed samples were centrifuged at 20,000×g for 2 min ...
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bioRxiv - Systems Biology 2020Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminant before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). 4-8 tubules were collected for each sample.
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated with the NucleoSpin Tissue kit (Macherey&Nagel, Düren, Germany) and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Pyrosequencing was performed on a PyroMark ID System (Biotage ...
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bioRxiv - Molecular Biology 2022Quote: The following kits were tested for the evaluation of the most effective RNA isolation: ZR Quick-RNA™ MicroPrep (catalogue #R1051, Zymo Research, CA,.), RNeasy® Micro Kit (catalogue #74004 ...
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bioRxiv - Microbiology 2022Quote: ... and the desired 4671 bp and 7177 bp fragments containing either RM_p16 to 20 (pRM bp 14561-19231) or RM_p16 through 21 (pRM bp 14561-21646) were gel-purified (Zymoclean ™Gel DNA Recovery Kit, Zymo Research, Irvine, CA). The fragment ends were blunted (DNATerminator® ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA from archived (stored at -80°C) American alligator whole blood was isolated using the Quick-RNA Whole Blood Kit (Zymo Research, Irvine, CA). Samples from both LW and CFR were selected randomly based only on the availability of samples ...
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA samples were prepared for targeted sequencing with the Quick-16S NGS library Prep kit and 16S primer set V3-V4 (Zymo Research Irvine, CA). The final library was sequenced on Ilumina MiSeq with a V3 reagent Kit (600 cycles) ...
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bioRxiv - Bioengineering 2022Quote: Yeast competent cells were transformed with polycistronic constructs containing fluorescent proteins using the Frozen-EZ yeast transformation II kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... was used for bacterial 16S ribosomal RNA targeted sequencing and custom 16S primers were utilized to amplify the V3-V4 region (Zymo Research, Irvine, CA). Real-time PCR was then used to prepare the sequencing library and final qPCR fluorescence readings were pooled together according to equal molarity and the final pooled library was cleaned using the Select-a-Size DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Genomics 2022Quote: Up to 75 ng of sheared gDNA was subjected to bisulfite conversion using the EZ-96 DNA Methylation Kit (Zymo Research; Irvine, CA), with liquid handling on a Hamilton MicroLab STAR (Hamilton ...
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bioRxiv - Cell Biology 2022Quote: ... similar to previous TRIM25 siRNA data.20 A 600-bp amplicon flanking the gRNA targeting site was amplified from genomic DNA isolated from each clonal population using a Quick-DNA Miniprep Plus kit (Zymo Research, Irvine, CA) and KOD Hot Start Master Mix (Millipore Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast cells in mid-log phase were co-transformed with pDEST32/pDEST22::AtNHR2B or pDEST32::HopG1/ pDEST22::AtNHR2B using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). Transformed yeast cells were plated on Double Drop Out (DDO ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the subset of 12 samples utilized for metagenomics using the Zymobiomics DNA/RNA Mini Kit (Zymo Research, CA, USA) and RNA was cleaned ...
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bioRxiv - Microbiology 2021Quote: ... 50 µl of apical wash was used for the extraction of nuclease digestion-resistant viral RNA using the Quick-RNA Viral kit (#R1035; Zymo Research, Irvine, CA), as described previously (36) ...
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bioRxiv - Microbiology 2021Quote: Double-stranded DNA was then purified from the lysates using a Zymo Genomic DNA Clean & Concentrator Kit-10 (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2021Quote: ... which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research, Irvine, CA). An analysis of our final DMS data suggest this library contains at least 7,614 of 7,686 possible variants ...
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bioRxiv - Microbiology 2022Quote: ... Triplicate DNA extracts from each sample were then pooled and DNAs were purified using DNA Clean & Concentrator Kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from AsPC1 or OV90 cells transfected with 100 nM siSMNDC1 or siCON for 48 h was extracted using Quick-RNA MiniPrep (R1055, Zymo Research, Irvine, CA). Three biological repeats were performed ...
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bioRxiv - Microbiology 2020Quote: ... The YAC DNA was extracted using spin column-based nucleic acid purification (ZR BAC DNA Miniprep Kit, ref. D4049, Zymo Research, Irvine, CA). The YAC containing viral cDNA was transformed into E ...
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bioRxiv - Synthetic Biology 2020Quote: ... MCs were further cleaned to remove any PP contamination using Plasmid-Safe ATP Dependent DNase kit (Epicenter, WI, USA) followed by the DNA Clean & Concentrator Kit (Zymo Research, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... The pellet was frozen at -20°C until DNA extraction using a Zymo fecal/soil DNA extraction kit according to enclosed directions (Zymo Research, Irvine, CA). After sonication ...
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bioRxiv - Genomics 2021Quote: ... Microbial DNA was extracted and purified after return to the lab with a Zymo Soil/Fecal DNA extraction kit (Zymo Research, Irvine, CA). Quantification of DNA was performed with a Qubit 3.0 fluorimeter (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Physiology 2021Quote: ... Individual frozen tissue samples (∼10 mg) were homogenized in TRI Reagent and total RNA was isolated using Direct-zol RNA MiniPrep kits (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA and DNA were extracted from the mosquito supernatants using the ZR-DuetTM DNA/RNA MiniPrep kit (Zymo Research Corp., Irvine, CA, USA) with a scaled down version of the manufacturer’s protocol with Dnase treatment of RNA using either the kit’s DNase or the Qiagen RNase-Free DNase Set (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
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bioRxiv - Immunology 2020Quote: ... The brush is removed from the channel of the bronchoscope and the brush is placed into 200 μL of DNA/RNA Shield X1 (Cat.# R1200, Zymo Research, Irvine, CA). The bronchoscope remains in place while two aliquots of 20 ml of saline each are instilled into the bronchus and subsequently aspirated ...
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bioRxiv - Microbiology 2020Quote: ... isolates were grown shaking in SG broth at 37°C and genomic DNA isolated using the Quick-DNA Fungal/Bacterial miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's suggested protocol.
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and viral RNA in the cell supernatants were extracted by using Direct-zol RNA MiniPrep kit (Zymo research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted from the resuspended pellet using the Zymo Quick-DNA Microprep Kit (cell suspensions protocol) per manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The DNA was quantified using the dsDNA kit on a Qubit 2.0 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellets were washed with PBS before total RNA was extracted with the Direct-zol RNA Micro-Prep method (Cat. R2063-A, Zymo Research Corp., CA), and the extracted RNA was reverse transcribed using LunaScript® RT SuperMix Kit (Cat ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μL of each reaction product were pooled and purified using the Zymo DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing was performed at the Chan Zuckerberg BioHub facility on an Illumina NextSeq 550 platform in High Output mode.
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid and fosmid DNA was purified with either the Zyppy Plasmid Miniprep kit or ZymoPURE II Plasmid Midiprep kit (Zymo Research, Irvine, CA). Following restriction digests or PCR ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was isolated from polarized murine bone marrow-derived macrophages using the Quick-RNA™ MiniPrep Kit (Zymo Research, Irvine, CA, USA). RNA quantity and quality was verified by two methods ...
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bioRxiv - Physiology 2022Quote: ... Samples thawed and then disrupted with an eppendorph tube pestle and total RNA purified using a Direct-zol plus RNA miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: HepG2 cell samples were collected into QIAzol Lysis Reagent and total RNA was extracted using Direct-zol RNA Miniprep (Zymo Research, CA, USA). Liver samples were first homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... 2016) Genomic DNA (500ng) from whole blood underwent bisulfite treatment with the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4 µl of bisulfite-converted DNA was measured on the Illumina 450k array following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and urine samples underwent RNA extraction in the Virology Unit at the Institut Pasteur de Madagascar (IPM) using the Zymo Quick DNA/RNA Microprep Plus Kit (Zymo Research, Irvine, CA). In total ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA (500ll!ng) from whole blood was bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), following the standard protocol for Illumina 450K micro-arrays ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...