Labshake search
Citations for Zymo Research :
1151 - 1200 of 1620 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cross-links between the DNA fragments and histones were reversed and the DNA fragments were recovered using the ChIP DNA Clean & Concentrator Kit (Zymo, Irvine, CA). Two biological replicates were performed for each group ...
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bioRxiv - Systems Biology 2022Quote: ... 100 μl of the diluted culture was spread on LB agar plates supplemented with 25 μg/ml of 2,3,5-triphenyltetrazolium chloride to stain colonies bright red using Rattler Plating Beads (Zymo Research, Irvine, CA, USA), and incubated for 12 to 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... The MDA-amplified VLP DNA was then processed using the Zymo Genomic DNA Clean and Concentrator-10 kit (Zymo Research, CA, U.S.A) and eluted in Zymo DNA Elution Buffer ...
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bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA from the heat lysed ZeptoMetrix MTB verification panel samples was purified and eluted in 40μL TE using Oligo Clean & Concentrator (Cat# D4061, Zymo Research, CA, USA). 5μL of extracted DNA was used as input for the LAMP assay.
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bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2023Quote: ... DNA bands at ∼380 bp were excised and purified utilizing the Zymo Gel DNA Recovery Kit (Zymo Research, Irvine, CA, United States). Purified PCR products were equimolar pooled for a final library concentration of 11 pmol/l ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from the livers or adipose tissue using Direct-zol RNA Microprep Kits (R2061, Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Swabs for microbiome analysis of the wound edge and center were collected using Levine’s technique and placed into 300 μl of DNA/RNA Shield (Zymo Research, Irvine, CA) and stored at –80°C until further processing ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Genomics 2023Quote: ... extracted RNA was concentrated and treated with DNAse I utilizing on-column digestion following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The resulting RNA was depleted of ribosomal RNA for two hours ...
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from thallus fragments using the ZR Fungal/Bacterial DNA Miniprep Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... was used as a positive control for each DNA extraction and the ZymoBIOMICS® Microbial Community DNA Standard (Zymo Research, Irvine, CA) was used as a positive control for each targeted library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting DNA was purified using the ChIP DNA clean concentrator as per the manufacturer’s instructions (Zymo Research, Irvine, CA, United States) and stored at -80°C until further analysis by (qRT-PCR) ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial samples were subjected to RNA extraction according to the manufacturer’s protocol (Zymo Quick-RNA Fungal/Bacterial Kits, Zymo Research, Irvine, CA). After a purity check of RNA extract quality using nanodrop and Agilent 5200 Fragment Analyzer (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was extracted from the brain tissues using Isogen reagent (Nippon Gene) and treated with DNase I (Zymo Research, Irvine, CA). Total RNA was then subjected to RT-PCR and amplicon sequencing to detect repeating units of wraparound transcripts as described above ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Genomics 2024Quote: ... 1987) and purified using a Zymo-Spin I-96 Plate and ZR-96 Clean and Concentrator Kit (Zymo Research Corporation, CA, USA) following the protocol of Rutherford et al ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from the leaves of soil-grown Arabidopsis plants using Direct-zol RNA MiniPrep Plus (Zymo Research, CA, USA) following manufacturer’s instructions and quantified using a NanoDrop spectrophotometer ...
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bioRxiv - Genomics 2024Quote: Viral RNA was extracted from the swab samples using the Zymo Quick-DNA/RNA Viral MagBead Kit (Zymo Research, Orange, CA, USA), and qPCR was performed using the AgPath-ID One Step RT-PCR Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: RNA was extracted according to protocol using Zymo Quick-RNA Fecal/Soil Microbe Microprep Kit (Cat# R2040, Zymo Research, Irvine, CA, USA). Samples were lysed by bead beating using Vortex Genie-2 set at max speed for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from previously collected and deidentified paraffin embedded PV patient biopsies using the Quick-RNA FFPE MiniPrep kit (ZR1008, Zymo, Irvine, CA). RNA concentration and purity were assessed using spectrophotometry ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was isolated using a modified phenol-chloroform extraction method (Davies et al., 2013) and then cleaned with DNA Clean and Concentrator kits (Zymo, Irvine, CA). Cleaned genomic DNA was sent to the University of California Davis Genome Center for library preparations using pooled Super-High-Throughput Shotgun sequencing for shallow WGS (mean 6x coverage ...
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bioRxiv - Microbiology 2024Quote: ... The fragments were stored underwater in individual Ziploc bags and preserved in tubes with DNA/RNA shield (Zymo Research, Irvine, CA, USA) at −20 °C once on board the schooner Tara ...
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bioRxiv - Genomics 2024Quote: Total DNA was extracted from each organ using the Quick DNA Fecal/Soil Microbe Miniprep kit (Zymo Research, CA, USA, Cat. D6010) following the manufacturer’s recommendations ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Digested plasmids were run in a 1.2% agarose gel and the ACE2 band was excised and purified using the Zymo Gel Extraction kit (Zymo Research, Orange, CA). Purified DNA was submitted to the University of Wisconsin-Madison Biotechnology Center DNA Sequencing Facility and a sequencing library was prepared using the NEBNext Ultra II kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... vSMC were cultured in a 12-well culture plate and lysed using 300 µl RNA lysis buffer (Zymo Research, Irvine, CA, USA). RNA was isolated with the Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were directly lysed in 1 ml of Trizol (R2050-1, Zymo Research) and subjected to interface cleanup as described below.
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bioRxiv - Molecular Biology 2023Quote: ... Round 1 PCRs were cleaned using DNA Binding Buffer (Zymo ZD4004-1-L) and UPrep Micro Spin Columns (Genesee Scientific 88–343) ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Physiology 2024Quote: ... the column was brought to room temperature for 5 min and the RNA was spun out into fresh tubes (50 μL for Zymo-II and 60 μL for Zymo-IICG columns). Next ...
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bioRxiv - Immunology 2021Quote: ... was used to isolate viral RNA (vRNA) from mucosal swab and bronchial brush samples collected in 200 µL DNA/RNA Shield 1X (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of buffy coat DNA was hybridized to the Methyl450 array after bisulfite conversion with EZ DNA kits (Zymo Research, Irvine, CA) prior to standard Illumina amplification ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Microbial DNA extractions were performed in batches (stored on ice) immediately after intestinal dissections with the Zymobiomics DNA Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Molecular Biology 2020Quote: Basal cells or well-differentiated epithelia were treated with Trizol and RNA was isolated using the Zymo Directzol Miniprep isolation kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Bioengineering 2020Quote: Total DNA was extracted from fecal and mucosal samples preserved in DNA/RNA Shield (DRS) solution (R1100-250, Zymo Research, Irvine, CA, USA) or fresh using ZymoBIOMICS DNA Miniprep Kit (D4300 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from dissected tissues using the Zymo Quick-DNA Miniprep plus kit following the solid tissue protocol (Zymo Research; Irvine, CA). The presence of wCfeT ...
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bioRxiv - Genomics 2021Quote: ... Bisulfite conversion of 700 ng genomic DNA was performed using EZ-96 DNA Methylation Kit (Deep Well Format) (Zymo Research; Irvine, CA, USA) in accordance with the given kit instructions (www.zymoresearch.com) ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 in DRG co-culture and 2 biological replicates of MDA-MB-231 in DRG-conditioned media (1 replicate was excluded during analysis after clustering revealed it was an outlier) was extracted 24h after co-culture using RNA MiniPrep Kit (R1057, Zymo Research, Irvine, CA). Samples were confirmed to have RIN value >8.5 with Agilent Bioanalyzer before undergoing library preparation with TruSeq stranded mRNA kit by Tufts Genomics Core ...