Labshake search
Citations for Zymo Research :
1001 - 1050 of 1090 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were purified from agarose gels using the Zymoclean® Gel DNA Recovery Kit or from PCR reactions using the DNA Clean & Concentrator®-5 DNA kit from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Cell Biology 2021Quote: ... and isolated genomic DNA was purified by using the Genomic DNA Clean & Concentrator™-10 kit (Zymo D4011). DNA was eluted in 25 µl of 10 mM Tris-HCl buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... About 10-50 ng mRNA of each fraction was converted using the EZ RNA methylation kit (Zymo Research) with a modified high-stringency conversion condition ...
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bioRxiv - Plant Biology 2024Quote: ... Final DNA purification was performed with the Zymogen Genomic DNA Clean and Concentrator-10 column (Zymo Research, Irvine). Libraries were prepared using SMRTbell Template Prep Kit 1.0 and sequenced on a PacBio Sequel platform at Novogene.
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bioRxiv - Biochemistry 2023Quote: ... 20 μL of the reaction mixture was added to 10 μL 0.5 M EDTA and purified by ZYMO Oligo Clean & Concentrator spin columns ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from whole-tissue (50 ng) and isolated mitochondria (10 ng) were converted to cDNA libraries (Zymo-Seq RiboFree Total RNA #R300 ...
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bioRxiv - Genomics 2024Quote: ... Creating biotinylated methylation controls involved initially combining 10 μl of fully methylated pUC19 DNA (Zymo Research, Cat.N: D5017) with an equal amount of unmethylated lambda DNA (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted from the cells grown in 10 cm dish using QuickRNA Miniprep kit (Zymo Research) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted DNA was treated with RNAse A (1 μL of 1 mg/mL added to samples of 100 μL for 30 minutes at room temperature) and then purified and concentrated using a DNA Clean and Concentrator-5 Kit (Zymo Research Corp., USA). The concentration of genomic DNA was measured on a QUBIT 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 % ethanol was added to the aqueous phase (1:1, v/v) and transferred to RNA Clean and Concentrator (RCC) ™ −5 kit (Zymo Research; R1013) and centrifuged at 16,000 x g for 30 s ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Genetics 2020Quote: ... Reactions were then quenched by the addition of 1 volume DTT 1 M and then purified on an RNA Clean & Concentrator-5 column (Zymo Research, cat. R1013).
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bioRxiv - Microbiology 2023Quote: ... Remaining material from the same DNA extract of subculture 19.9 was then concentrated using the DNA Clean and Concentrator-5 kit (Zymo Research; Irvine, California, U.S.A.) and used for long read sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was purified and the DNA oligos were removed from samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: For DNA extraction cells were collected via filtration and filters transferred to 5 ml cryo vials containing 1 ml DNA/RNA shield (Zymo Research, Irvine, CA) as preservative ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was removed from the purified RNA samples using RNA Clean & Concentrator™-5 columns with on-column DNase digest (Zymo Research, R1014). For rRNA-depleted samples ...
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bioRxiv - Molecular Biology 2022Quote: ... The solution was diluted to 10% RNALater by the addition of 270μL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 2.5 μl 10% SDS and purified using the DNA Clean & Concentrator Kit (Zymo). 20 μl of the transposed DNA was then amplified using the NEBNext High-Fidelity 2x PCR Master mix with i5 and i7 Nextera barcoded primers for 12 cycles (see Supplementary Table 6 for primer sequences) ...
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Dual symbiosis in the deep-sea hydrothermal vent snail Gigantopelta aegis revealed by its hologenomebioRxiv - Genomics 2020Quote: ... The extracted DNA was further purified by Genomic DNA Clean & Concentrator™-10 kit (ZYMO Research, Irvine, CA, USA). The DNA was finally eluted in 10mM Tris-HCl buffer (pH=8.5) ...
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bioRxiv - Developmental Biology 2024Quote: ... Bulk genomic DNA was extracted from 10 non-virgin female adult flies using the Quick-DNA Microprep kit (Zymo) and quantified by Qubit ...
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bioRxiv - Microbiology 2022Quote: ... total RNA (10 μg) was subjected to RNaseR digestion followed by cleanup using RNA clean and concentrator kit (Zymo). Linear RNA depleted fraction was then fragmented using Magnesium RNA fragmentation module using manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were first grown in YPD overnight and then streaked on SC agar plates containing 1 mg/mL 5-FOA (Zymo Research, Orange, CA, USA). A single colony from an SC/5-FOA agar plate was streaked again on a new SC/5-FOA agar plate ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA extractions were performed upon 5 million trophozoites of Nf69 using Quick-DNA Miniprep Plus Kit according to manufacturer recommendations (Zymo Research, Irvine, CA, USA). In short ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The sample was then centrifuged at 2300 rpm for 5 min and the supernatant was collected and concentrated and purified using a Zymo-Spin V reservoir (Zymo Research Irvine, CA, USA) and Qiagen MinElute spin column (Qiagen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... strains were struck to synthetic defined (SD) media (CSM minus arginine, yeast nitrogen base, glucose) containing 5-Fluoroorotic Acid (5FOA) (Zymo Research, Irvine, CA, USA). to isolate cells that spontaneously lost pTC416-SEC53 ...
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bioRxiv - Genomics 2023Quote: ... Tagmentation was carried out at 55C for 5 minutes before being stopped by the addition of 12 uL of DNA binding buffer (Zymo Research, D4004-1-L) for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Neuroscience 2023Quote: RRBS libraries were prepared from 200 ng of genomic DNA digested with MspI restriction enzyme and then extracted with ZR-DNA Clean & Concentrator™-5 kit (Zymo Research, Cat# D4014). According to Illumina’s specified guidelines ...
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bioRxiv - Microbiology 2023Quote: ... a 4.5 ml sample of each microcosm and extracted bacterial DNA with a Quick-DNA Fecal/Soil Microbe Miniprep Kits (Zymo Research, Irvine, California, USA). Two negative controls that contained the SH salt medium alone were included in the process of DNA extraction ...
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bioRxiv - Bioengineering 2024Quote: Cell number was directly assessed as a function of total isolated DNA at experimental Days 1 and 7 using a Zymo Quick-DNA Microprep Plus Kit (n=5, Zymo Research Crop, Irvine, CA). A standard curve at experimental Day 0 was generated using known quantities of hMSCs and following the “Biological Fluids & Cells” protocol as directed ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
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bioRxiv - Cancer Biology 2020Quote: ... cfDNA was isolated from 0.5 - 10 ml of plasma using the Quick-cfDNA Serum & Plasma Kit centrifugation protocol according to the manufacturer’s protocol (Zymo Research). cfDNA was eluted twice in the provided elution buffer or in MilliQ and subsequently stored at −80°C ...
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bioRxiv - Genetics 2021Quote: ... 100µl of the gDNA solution was purified using the Zymo Genomic DNA Clean and Concentrator –10 kit (Zymo Research #ZD4010) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... of PCR-amplified parts from the genomic DNA of a phiX174 bacteriophage genome (Supplementary Table S4), purified from a 10% urea-PAA gel (RNA PAGE Recovery, (Zymo). Determination of RNA concentration was performed using the Qubit RNA HS Assay kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... ∼10% of both nuclear and cytoplasmic lysates were used for Western Blot and ∼90% subjected to RNA extraction (ZYMO R1055).
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bioRxiv - Genomics 2021Quote: Total RNA was purified from the 10 replicate cell cultures using a Direct-zol RNA Miniprep Plus kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mL of samples were centrifuge to separate the biomass (3,000 rpm, 10 min) and genomic DNA was extracted with the ZR Soil Microbe DNA MiniPrepTM kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from overnight LB agar cultures harvested and re-suspended in 5mL 10 mM phosphate buffer (pH 7.0) using the genomic DNA Quick Prep Kit (Zymo Research), and stored at -20°C ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries were prepared genomic DNA (10 ng) using a Pico Methyl-Seq Library Prep Kit (ZYMO, catalog # D5456). MQ beads (Epigentik ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was diluted to 10% RNALater by the addition of 270 µL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...