Labshake search
Citations for Zymo Research :
951 - 1000 of 1090 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... followed by a 5-day incubation period before total RNA was collected by Direct-zol RNA MiniPrep Plus kit (Zymo Research). 69 RNASeq profiles ...
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and mixed with 450 μl ethanol and transferred to an RNA Clean & Concentrator-5 column (Zymo Research) for downstream RNA purification and concentration ...
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was subsequently extracted with trizol/chloroform and cleaned with the Zymo RNA Clean and Concentrator-5 columns (Zymo Research, #R1013) with DNA digestion.
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bioRxiv - Microbiology 2024Quote: ... they were subjected to rRNA depletion using Ribominus rRNA depletion kit and further purified using RNA Clean and Concentrator-5 columns (Zymo Research). RNA integrity was checked using Bioanalyzer before proceeding to library prep and sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA pellets were resuspended in water and digested with 2 U of DNAse I for 30 min at 37 °C followed by purification using RNA clean & concentrator 5 columns (Zymo Research). mRNA levels in every fraction was quantified by RT-qPCR as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research). About 1.5 μg of total RNA was retrotranscribed with hexamers using Superscript VI retrotranscriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products for both pairs of primers were pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Cancer Biology 2024Quote: ... was incubated for 30 minutes on a rotating orbital.DNA recovered from dynabeads was purified with a DNA clean and Concentrator-5 kit (Zymo Research, D4013). Amplification of the recovered DNA was carried out using a whole genome amplification kit (WGA2 ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from 1 × 106 A549 cells treated with 0.5 % (v/v) DMSO or 5 µM FICD inhibitors (C22 or C73) for approximately 24 hours using an RNA miniprep kit (Zymo Research) and quantified using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Genomics 2022Quote: ... take out 10 μl and add 40 μl nuclease-free for second column purification (Zymo Research, R1016) followed by elution with 10 μl nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was purified in 10 μl water using the Oligo Clean and Concentrator™ kit (Zymo). Next ...
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bioRxiv - Genomics 2023Quote: ... The product was extracted from a 1% agarose-TBE gel (Zymo Gel Extraction kit, 10 µL elution), then assembled with the 5′ UTR gBlock in a Gibson reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 10-15 embryos were lysed and homogenized in 0.5 mL of TRI-Reagent (Zymo Research, Irvine, CA) with a pellet pestle ...
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using TRIzol-based (Fisher: 15596026) phenol-chloroform extraction and purified with RNA Clean and Concentrator-5 spin columns (Zymo research: R2050). RNA concentration and quality was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with 0.5 mg/ml proteinase K at 65 ℃ for one hour and purified with Zymo ChIP DNA Clean Concentrator kit (Zymo Research D5205) as the manufacturer’s manual and quantified by QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... The aqueous phase was retained for RNA purification using the Zymo RNA Clean & Concentrator-5 kit following manufacturer directions for the capture of small RNA fragments with slight modification (Zymo Research, R1013). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...