Labshake search
Citations for Zymo Research :
901 - 950 of 1036 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 100 ng of each sheared DNA sample was then processed using the EZ DNA Methylation Gold Kit or EZ DNA Methylation-Lightning Kit (Zymo Research) according to the manufacturer’s protocol to create bisulfite-converted single-stranded (BC-ss ...
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Pathology 2023Quote: ... The DNA samples were then prepared for targeted sequencing with Quick-16S Primer Set V3-V4 Library (Zymo Research, Irvine, CA). The sequencing library was prepared using innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and limit PCR chimera formation ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from temporal cerebral cortex samples using the Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo, Irvine, CA, USA) and purified using the RNA Clean and Concentrator-5 kit (Zymo) ...
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ng of spike-in transcript was added to each sample at this step before RNA was isolated using Zymo Direct-Zol RNA extraction columns (Zymo #R2052), and RNA integrity was assessed by the appearance of two sharp rRNA bands on a 1% agarose gel and quantified using the absorbance at 260 nm.
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of ethanol were added to each of the samples before eluting the RNA using a Direct-zol RNA kit (Zymo Research) using manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was isolated from rat kidney samples and PTC cultures using Trizol + Direct-zol columns according to the referenced protocol (Zymo Research). For PTC cultures ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from fecal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... Negative controls (sterile water treated as a sample) and one positive control (ZymoBIOMICS Microbial Community Standard, Zymo Research, Cat No. D6300) were included in each batch ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from TRAP and input samples with TriZol and the Direct-Zol RNA MicroPrep Kit (Zymo Research, Orange, CA).
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from these samples as previously described (19) using a ZymoBiomics DNA Miniprep kit (Zymo Research, Irvine, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 0.15-0.25 grams of stool samples were extracted using the Quick-DNA Fecal/Soil Microbe kit (Zymo Research, Irvine, CA, USA). Negative extraction controls were included to ensure that no exogenous DNA contaminated the samples ...
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bioRxiv - Genomics 2024Quote: ... one additional library per sample was constructed using the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, Irvine, CA, USA). Each library type was pooled and 150 bp paired-end reads generated on an Illumina NextSeq 550 instrument at the University of Saskatchewan Next-Generation Sequencing Facility ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of DNA from the validation cohort of 20 patients (20 adenoma and 20 nontumor tissue samples) was processed with the EZ DNA Methylation-Gold Kit following the standard protocol (Zymo Research). Bisulfite conversion was followed by amplification of the target CpGs ...
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bioRxiv - Developmental Biology 2024Quote: We isolated genomic DNA from all samples using the Zymo Quick DNA mini-prep Plus Kit (Zymo Research; Cat. no. D4068). The samples were amplified and prepared for sequencing as described previously (Gilbert et al ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in TRIzol buffer and RNA was isolated using RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Molecular Biology 2020Quote: ... Immediately following digestion the reaction was stopped in the binding buffer from a Zymo ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205) and purified following the protocol in the kit ...
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bioRxiv - Microbiology 2022Quote: ... and 250 μl of this cell suspension was added to 250 μl of lysis buffer in ZR BashingBead™ lysis tubes (Zymo Research) containing 0.1 mm and 0.5 mm beads ...
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bioRxiv - Genomics 2022Quote: ... separating 22 or 10 (control wells) single nuclei per well in a 96-well plate containing 2.5 μL of M-digestion buffer (Zymo, Cat. D5020-9), 0.25 μL of Proteinase K (Zymo ...
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bioRxiv - Biochemistry 2023Quote: ... The tagmented DNA was cleaned up using Zymo DNA Clean and Concentrator-5 columns with 5X binding buffer (Zymo, cat. no. D4004). 5 μL of purified DNA out of 12.5 μL purified product was amplified in a 25 μL PCR reaction containing NEBNext PCR master mix (1X final) ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-S-UTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research, R1013) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... vSMC were cultured in a 12-well culture plate and lysed using 300 µl RNA lysis buffer (Zymo Research, Irvine, CA, USA). RNA was isolated with the Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2020Quote: ... and then both ribosome-bound RNA and denatured total RNA samples were purified with RNA Clean & concentrator-25 kit (Zymo Research, USA). RPFs were size-fractionated on 15% urea polyacrylamide gel electrophoresis ...
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bioRxiv - Genetics 2021Quote: ... Per sample 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Genetics 2020Quote: 200ng of liver genomic DNA per sample was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Standards using mouse genomic DNA of known percentage methylation (0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from the pellets and the highly infected samples used the Zymo Quick-RNA kit (Zymo Research LLC, Irvine, CA). Three samples with unfiltered hemolymph ...
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bioRxiv - Genetics 2021Quote: ... The adaptor-ligated DNA samples were subjected to bisulfite conversion by the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA, USA) and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche ...
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bioRxiv - Microbiology 2021Quote: ... and 103 fecal samples were collected in June 2018 and stored using DNA/RNA Shield swab collection tubes (Zymo Research, Cat# R1107). The samples were gathered from a total of 71 individuals in 28 households ...
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bioRxiv - Systems Biology 2022Quote: ... Total DNA extractions were performed for tea broth and pellicle samples using Quick-DNA Fecal/soil Microbe Miniprep kit (ZYMO Research Corp.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted from flash-frozen fetal liver (left lobe) samples using a Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA)) per manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: ... and 500ng of DNA from each sample was bisulfite converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol using the specified modifications for the Illumina Infinium Methylation Assay ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The RNA was then extracted from both samples using the Quick-RNA MiniPrep kit following the manufacturer’s instructions (Zymo Research, Irvine, CA). Total RNA in samples was quantified using a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from the leaf samples with the Direct-zol RNA Miniprep Plus kit according to the manufacturer’s (Zymo Research Europe GmbH) guidelines ...
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 10 min at 37°C before quenching using a Zymo Genomic DNA Clean & Concentrator 10 (Zymo Research D4011) kit according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA from the heat lysed ZeptoMetrix MTB verification panel samples was purified and eluted in 40μL TE using Oligo Clean & Concentrator (Cat# D4061, Zymo Research, CA, USA). 5μL of extracted DNA was used as input for the LAMP assay.
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bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: Oropharyngeal swabs and stool samples were collected in collection tubes containing DNA/RNA shield (Zymo Research Cat# R1107-E and Cat# R1101) and frozen at -80C until further analysis was performed ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...
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bioRxiv - Systems Biology 2023Quote: DNA was extracted from approximately 100 mg of each gut content sample using the ZymoBIOMICS™ 96 MagBead DNA Kit (D4308) (Zymo Research), following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... These samples were used as the starting material for an RNA extraction using the Quick-RNA Viral Kit (Zymo, Cat no: R1034). The final elution was collected in 15 μl of nuclease-free water.