Labshake search
Citations for Zymo Research :
851 - 900 of 1036 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: cDNA preparation and preamplification: RNA isolation of digested samples was performed using the Quick-RNA MicroPrep kit (Zymo Research, Cat #: R1050). In-column DNAse-I treatment was performed for trace DNA removal before eluting RNA ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Genomics 2020Quote: ... We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200) the resulting library.
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bioRxiv - Immunology 2021Quote: ... triplicate cell samples or 50 uL fresh mouse blood were harvested into Trizol reagent before purification via Direct-zol RNA miniprep (Zymo Research) per manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent carryover ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse cross-linked samples were treated with 20 μg/ml Proteinase K and purified using ChIP DNA Clean and Concentrator kit (Zymo research). The three reactions per condition were pooled at this stage ...
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bioRxiv - Genomics 2020Quote: Total RNA from testicular tissue samples of the four bulls was extracted using Direct-zol RNA Mini Prep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from C2C12 myoblasts or postnatal day 21 tail tip samples using Direct-zol RNA miniprep kit (Zymo Research) and reverse-transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... DNA from the ChIP and input control samples was isolated using ChIP DNA Clean and Concentrator Kit (Zymo Research, Irvine, CA) to prepare sequencing libraries using NEBnext Ultra II kit (NEB) ...
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bioRxiv - Plant Biology 2020Quote: Frozen leaf disc samples were ground with ball bearings and extracted using Direct-zol™-96 RNA (Zymo Research; Irvine, CA). Total RNA was eluted in DNAse/RNAse free water ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... and DNA extraction of 2017 surface precipitate samples was extracted using the ZymoBIOMICS™ DNA/RNA Mini Kit (Zymo Research Corp.). All extractions were performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from TRI Reagent samples using phenol-chloroform extraction or column-based extraction systems (Direct-zol RNA Microprep and Miniprep, Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from TRI Reagent samples using phenol-chloroform extraction or column-based extraction systems (Direct-zol RNA Miniprep, Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from TRI Reagent samples using phenol-chloroform extraction or column-based extraction systems (Direct-zol RNA Miniprep, Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified from proteinase K treated samples using a DNA purification kit following the manufacturer instructions (Zymo Research, CA, USA) and was subsequently analyzed either by running a 2% low melting agarose (APEX ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: Swab and bronchial brush samples were collected in 200 μL of DNA/RNA Shield 1x (Cat.# R1200, Zymo Research, Irvine, CA) and extracted for Viral RNA (vRNA ...
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bioRxiv - Microbiology 2020Quote: ... The nuclease-treated samples were used for viral RNA extraction using the Viral RNA extraction kit (Quick-RNA Viral Kit, R1035, Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Microbiology 2021Quote: ... DNA used for V4 sequencing was extracted from each sample using the Zymo Quick-DNA Fecal/Soil Microbe 96 Kit (Zymo D6011) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of this soma sample was then used for RNA isolation using the Zymo QuickRNA MicroPrep kit (Zymo Research, #R1050). RNA was also isolated from the 500 μL neurite lysate using the same kit ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth from each treatment condition was collected and pooled into a three samples and genomic DNA was extracted using Zymobiomics DNA miniprep kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from ∼1g of sample using Zymo quick DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Cat No. D6010) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
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bioRxiv - Immunology 2020Quote: Swab and bronchial brush samples were collected in 200 μL of DNA/RNA Shield X1 (Cat.# R1200, Zymo Research, Irvine, CA) and extracted for Viral RNA (vRNA ...
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bioRxiv - Genetics 2020Quote: ... We then prepared whole-genome bisulphite sequencing libraries from each DNA sample using the Pico Methyl-Seq Library Prep Kit (Zymo Research), according to the manufacturer’s protocol (see Supplementary Table 1 for detailed sample metadata and sequence accession codes) ...
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bioRxiv - Genomics 2021Quote: ... Samples were bisulfite converted after size selection using the EZ-96 DNA Methylation-Gold Kit (cat#: D5008, Zymo, Irvine, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The rRNA-depleted RNA samples were purified using the Zymo RNA Clean & Concentrator kit combined with DNase treatment on a solid support (Zymo Research). cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 µL of the sample was transferred to a fresh tube and RNA was extracted using a Direct-zol RNA microPrep kit (Zymo Research). All RNA samples were treated to remove DNA using a DNase Treatment and Removal kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA from the input control and IP samples was isolated using the ChIP DNA Clean and Concentrator Kit (#D5205, Zymo Research) according to manufacturer’s guidelines ...
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bioRxiv - Pathology 2023Quote: ... The DNA samples were then prepared for targeted sequencing with Quick-16S Primer Set V3-V4 Library (Zymo Research, Irvine, CA). The sequencing library was prepared using innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and limit PCR chimera formation ...
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bioRxiv - Genomics 2023Quote: Five Kellet’s whelk individuals were dissected for the Qiagen and Zymo kit extractions with each sample having a duplicate (10 total extractions conducted using the Qiagen kit and 10 total extractions conducted using the Zymo kit) in March 2021 ...
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bioRxiv - Microbiology 2023Quote: Metagenomic DNA and RNA were extracted starting from 350 µL of DNA/RNA shield swab sample (previously vortexed) and following a modified protocol of ZymoBIOMICS DNA/RNA Miniprep kit (Zymo Research) in which the lysis was performed only chemically using 700 µL of ZymoBIOMICS DNA/RNA lysis buffer (2 volumes of lysis buffer per 1 volume of sample) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Microbiology 2024Quote: ... and the total RNA of each sample was isolated with the Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from samples collected at the 48h timepoint (72h after the transfection) using Quick-DNA/RNATM Microprep Plus Kit (Zymo Research) including the DNAse I step ...
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bioRxiv - Microbiology 2024Quote: ... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The total microbe DNA from 100 mg of bulk or rhizosphere samples was extracted using the Quick-DNATM Fecal/Soil Microbe Miniprep kit (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Total genomic DNA from 50mg of dried samples and a standard microbial community (zymoBIOMICS Microbial Community Standard D6300, by ZYMO RESEARCH), here named “Ze” ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from temporal cerebral cortex samples using the Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo, Irvine, CA, USA) and purified using the RNA Clean and Concentrator-5 kit (Zymo) ...
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bioRxiv - Evolutionary Biology 2024Quote: We used a Trizol-based protocol to extract RNA from primate samples using the Direct-zol RNA Microprep kit (R2060, Zymo Research). All steps were centrifuged at 13,000 g for 30 s unless otherwise noted ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume of ethanol was added to the sample and RNA is isolated using the Direct-zol kit (Zymo research, R2050). The RNA eluted from the column was treated with TURBO DNase kit (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was isolated from biological triplicates of XX and XXY ovaries (60/sample) by Direct-zol miniprep kit (Zymo Research). Reverse transcription and qPCR were performed following the manufacturer’s protocol using TaqMan MicroRNA Reverse Transcription Kit ...
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bioRxiv - Genetics 2022Quote: ... An amount of 200 ng of genomic DNA from each sample underwent bisulfite conversion with Zymo DNA Methylation Gold Kit (Zymo Research). The converted DNA served as a template for PCR amplification and library preparations as in the former section ...
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bioRxiv - Microbiology 2022Quote: The samples were processed and analyzed with the ZymoBIOMICS®Shotgun Metagenomic Sequencing Service for Microbiome Analysis (Zymo Research, Irvine, CA).