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Citations for Zymo Research :
901 - 950 of 1068 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The digested fragments were purified using the DNA Clean & Concentrator-5 Kit and the Zymoclean Gel DNA Recovery Kit (Zymo Research) respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Products were checked on an agarose gel and cleaned-up through a PCR-clean up kit (DNA Clean and Concentrator-5, Zymo, D4013), followed by a secondary PCR to add illumina indexes (SFig ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by a 5-day incubation period before total RNA was collected by Direct-zol RNA MiniPrep Plus kit (Zymo Research). 69 RNASeq profiles ...
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bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2024Quote: ... Products were checked on an agarose gel and subjected to a PCR-clean up step (DNA Clean and Concentrator-5, Zymo, D4013). A second PCR (PCR2 ...
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... or downstream of the poly(A) segment (Trichoplax AKDF19383) and purified via ethanol precipitation or DNA Clean & Concentrator-5 columns (Zymo Research). mRNA was transcribed for 2-2.5 h with mMESSAGE mMACHINE kits (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The aqueous phase was extracted and cleaned up using the Zymo RNA Clean and Concentrator-5 kit (Zymo Research, cat#R1013). Only samples with RNA integrity (RIN ...
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bioRxiv - Microbiology 2024Quote: ... All 30 samples were pooled in an equivolume and purified with RNA Clean & Concentrator-5 columns (Zymo Research, Irvine, CA, USA). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
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bioRxiv - Cell Biology 2023Quote: ... Viral gene sequences in pPolI plasmids for all PR8 segments were amplified by PCR using corresponding primer pairs and were purified using ZYMO Research DNA cleaner and Concentrator-5 (ZYMO, D4014). Purified PCR products were in vitro transcribed using the T7 RiboMAX Express Large Scale RNA Production System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Cancer Biology 2023Quote: ... Initial genomic library distributions were assessed on a 1.5% agarose gel and purified using a ZR-96 Genomic DNA Clean & Concentrator-5 Kit (Zymo Research, D4067). Library concentrations were quantified using the Quant-iT™ dsDNA Broad Range Assay Kit (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and mixed with 450 μl ethanol and transferred to an RNA Clean & Concentrator-5 column (Zymo Research) for downstream RNA purification and concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... the non-poly(A) RNA segments in the flow-through were purified using the RNA Clean & Concentrator-5 kit (Zymo Research). The resulting purified poly(A ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Neuroscience 2023Quote: ... 88∼135 bp) were resolved on a 10% TBE polyacrylamide gel and extracted separately using the DNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Microbiology 2023Quote: RNA was purified and concentrated according to the protocol for RNA Clean and Concentrator Kit - 5 (Zymo Research Corporation, Irvine, CA) with an eluted volume between 30-35µL ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified by phenol-chloroform extraction and the aqueous fraction was further cleaned using DNA Clean & Concentrator-5 with Zymo-Spin Columns (Zymo Research). Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA pellets were resuspended in water and digested with 2 U of DNAse I for 30 min at 37 °C followed by purification using RNA clean & concentrator 5 columns (Zymo Research). mRNA levels in every fraction was quantified by RT-qPCR as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research). About 1.5 μg of total RNA was retrotranscribed with hexamers using Superscript VI retrotranscriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products for both pairs of primers were pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Microbiology 2024Quote: ... they were subjected to rRNA depletion using Ribominus rRNA depletion kit and further purified using RNA Clean and Concentrator-5 columns (Zymo Research). RNA integrity was checked using Bioanalyzer before proceeding to library prep and sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was subsequently extracted with trizol/chloroform and cleaned with the Zymo RNA Clean and Concentrator-5 columns (Zymo Research, #R1013) with DNA digestion.
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bioRxiv - Cancer Biology 2024Quote: ... was incubated for 30 minutes on a rotating orbital.DNA recovered from dynabeads was purified with a DNA clean and Concentrator-5 kit (Zymo Research, D4013). Amplification of the recovered DNA was carried out using a whole genome amplification kit (WGA2 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from 1 × 106 A549 cells treated with 0.5 % (v/v) DMSO or 5 µM FICD inhibitors (C22 or C73) for approximately 24 hours using an RNA miniprep kit (Zymo Research) and quantified using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: Total RNA isolations were performed from SARS-CoV-2 specimens using DIRECT-ZOL RNA Miniprep Kits (Zymo Research, USA). Quantitative RT-PCR was performed with the QUANTIVIRUS SARS-CoV-2 Test Kit (Diacarta ...
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bioRxiv - Immunology 2021Quote: Total RNA isolations were performed from SARS-CoV-2 specimens using Direct-zol RNA Miniprep Kits (Zymo Research, USA). Quantitative RT-PCR was performed with the QuantiVirus SARS-CoV-2 Test Kit (Diacarta ...
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bioRxiv - Genomics 2021Quote: ... Excess positive SARS-CoV-2 inactivated swab samples (200µl viral transport medium from nose and throat swabs inactivated in 200 µl Zymo DNA/RNA shield and 800 µl Zymo viral DNA/RNA buffer ...
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bioRxiv - Systems Biology 2023Quote: ... We thawed the cell pellets on ice and then resuspended with 400 µL RNA binding buffer (Zymo, #R1013-2). The mixtures were then transferred to 2 mL screw cap tubes with zirconia beads inside ...
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bioRxiv - Molecular Biology 2024Quote: ... which were cut out from a 2% agarose gel and cleaned using the Zymoclean Gel DNA Recovery Kit (Zymo), eluting in 15 μl ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...