Labshake search
Citations for Zymo Research :
851 - 900 of 1068 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... P7 and E18.5 was directly dissolved in RNA isolation buffer and RNA was isolated using the Quick-RNA micro prep kit (Zymo). Library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit ...
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from ∼6x105-7x105 MRC-5 infected fibroblasts using Zymo Duet RNA/DNA isolation Kit (Zymo Research). Viral genomes were quantitated by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification and plasmid isolation was done using the DNA Clean & Concentrator-5 and Zyppy Plasmid Miniprep Kits from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted and purified using Trizol reagent and RNA Clean & Concentrator-5 kit (Zymo Research cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Genomics 2023Quote: ... the aqueous phase was transferred to an RNA Clean and Concentrator-5 column and purified according to the manufacturer’s instructions including on-column DNaseI digestion (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Cell Biology 2022Quote: ... all wells were pooled to purify DNA products using the Zymo DNA Clean & Concentrator-5 kit (Zymo, Cat #: 11-303) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Microbiology 2024Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
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bioRxiv - Biochemistry 2024Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Immunology 2024Quote: ... and the resulting products were purified using the DNA Clean and Concentrator-5 Kit following the manufacturer’s instructions (Zymo Research). In-Fusion reactions were prepared in 5 µL volumes at a 1:3 vector to insert molar ratio ...
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bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Molecular Biology 2024Quote: ... a final round of PCR was performed by taking 100 ng of amplified sgRNA library and 5 cycles of the above PCR program was run followed by Zymo column clean up ...
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bioRxiv - Bioengineering 2024Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Systems Biology 2020Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Plant Biology 2021Quote: ... 500 pg - 2 µg DNA was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research). Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1 ...
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bioRxiv - Genomics 2021Quote: ... RNA was isolated from 2 day-differentiated TXΔXicB6 cells using the Direct-Zol RNA Miniprep Kit (Zymo Research). To remove any remaining gDNA ...
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bioRxiv - Immunology 2021Quote: Total RNA isolations from SARS-CoV-2 were carried using DIRECT-ZOL RNA Miniprep Kits (Zymo Research, USA), and concentrations were determined using QUBIT fluorometer with the QUBIT RNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 2 x 500 ng of DNA was bisulfite modified with the EZ DNA Methylation-Lightning kit (\#D5030, Zymo) and standardized to a concentration of 25 ng/uL BC-DNA ...
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bioRxiv - Systems Biology 2023Quote: ... We then sequentially added and centrifuged through the column 400 µL DNA/RNA prep buffer (Zymo, #D7010-2), 600 µL DNA/RNA wash buffer (Zymo ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 2×106 NCI-H295R cells was isolated with the Quick-DNA Miniprep Kit (Zymo research). Bisulfite treatment was performed using the EZ DNA Methylathion™ Kit (Zymo Research) ...
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bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 min at 30Hz and extracted RNA using the Direct-Zol RNA MiniPrep kit (Zymo Research, USA) with on-column DNA digest according to the manufacturer protocol ...
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bioRxiv - Genomics 2024Quote: The PCR reaction contained 2 µl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 µl of buffer + 0.05 µl of Taq Polymerase (PCRBIO Classic Taq ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Genetics 2021Quote: ... 5 heads of each genotype were pooled and total RNA extracted using the Direct-zol RNA Microprep kit (Zymo Research, R2060) and the quality determined using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2020Quote: ... all PCR products were cleaned using a Zymo Research ZR-96 DNA Clean & Concentrator™-5 (Zymo Research, Irvine, California, USA). Samples were eluted in 50µl of DNA free water.
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 24 hours to ensure complete removal of any 5’-triphosphate moieties then purified again using a RNA spin column (Zymo Research). Lastly ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared supernatant was then transferred to a fresh reaction tube and applied to spin-columns (RNA Clean & Concentrator-5 columns, Zymo-Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...