Labshake search
Citations for Zymo Research :
8551 - 8600 of 9639 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Bisulfite conversion of the methylated adapter-ligated DNA fragments was achieved using the EZ Methylation-Gold kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... and the resulting PCR products were purified (Zymo, Irvine,CA), and sequenced (GENEWIZ ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was sheared via sonication on the Covaris platform prior adaptor ligation and bisulfite conversion using the EZ-96 DNA Methylation-Lightning Kit (Zymo®). Libraries were then amplified using the DNA UltraII Kit (NEB®) ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA was extracted using Zymo Direct-zol kit (Zymo Research). RNA concentration was measured with Qubit RNA HS assays (ThermoFisher #Q10213 ...
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bioRxiv - Plant Biology 2020Quote: ... Constructs were transfected into yeast using Frozen-EZ Yeast Transformation II Kits (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Fragmented DNA was purified using a Zymo clean and concentrator column (Zymo Research) following the manufacturer’s instructions and eluted into 11 μl of TLE (10 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Blunted fragments were purified using a Zymo clean and concentrator column (Zymo Research) following the manufacturer’s instructions (eluted into 8 μl of TLE ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Some DNA extracts that showed high levels of degradation were size-selected using Select-a-Size column or bead kits (ZYMO, Irvine, CA). High molecular weight ...
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bioRxiv - Biochemistry 2020Quote: ... DNA was released by treatment with Proteinase K and purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). DNA yield was approximately 1-2 ng/107 cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were column-purified (Zymo Research, Irvine, CA) and eluted in 30 μL ...
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bioRxiv - Neuroscience 2020Quote: ... We recovered the DNA using ChIP DNA Clean & Concentrator columns (Zymo Research, Irvine, CA), and stored the resulting material at −80 °C before qPCR analysis ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA was Bisulfite-converted (BC) using the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research), and assessed using qPCR with optimized primers and SsoAdvanced SYBR Green Master Mix (BIO-RAD) ...
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bioRxiv - Neuroscience 2020Quote: ... RNA extraction was performed using the Zymo Directzol Microprep RNA Kit (Zymo; Cat#: R2062). RNA quality was evaluated using an Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were treated with DNaseI (Zymo Research) or TURBO DNase (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We pooled up to three extractions per sample using the Genomic DNA clean & Concentrator kit (Zymo Research, Irvine, CA, USA), following the supplied protocol ...
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bioRxiv - Cell Biology 2020Quote: ... DNase-treated samples were then further purified and concentrated using an RNA-cleanup kit (Zymo Research), and cDNA was obtained using SuperScript III first-strand synthesis (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... reagent using a bead mill apparatus (MPBio 5G, Irvine, California, USA) followed by column purification (Zymo Direct-zol RNA mini prep ...
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bioRxiv - Neuroscience 2020Quote: ... TUNEL positive control experiment was performed using DNase LJ (ZYMO RESEARCH, #E1010)-treated brains.
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated using the Mini Kit (Zymo Research) and sent to Novagene for library preparation and sequencing.
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bioRxiv - Microbiology 2020Quote: ... and total RNA was isolated by either phase separation with chloroform and isopropanol or using the Zymo Direct-zol RNA Miniprep kit (Zymo Cat #R2050). One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200 ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Microbiology 2020Quote: ... Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e. 1X PBS, pH 7.2 or Zymo DNA/RNA shield). Swabs in PBS were placed on ice and sent for culturing while the other swabs were transported back at room temperature to the laboratory and stored at -80°C ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was eluted from all samples using the Direct-zol kit (Zymo Research) and cDNA libraries were prepared with total RNA Gold library preparation (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... The RNA was further purified and DNase treated using the Direct-zol RNA MiniPrep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... The RNA was then column purified according to the manufacturer’s instructions (Zymo, R1019). PCR with primers specific to intron 1 and exon 3 from the mouse β-actin gene were used to screen samples for genomic DNA after DNase treatment.
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bioRxiv - Bioengineering 2020Quote: ... pelleted and plasmid DNA was extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... as follows: A purified α-factor pheromone suspension (Y1001, Zymo Research, Irvine, CA) was diluted to a working concentration of 10 μM in 100 mM KCl to achieve and maintain saturation binding of ligand to receptors present within sample cell membranes59–60 ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were then purified (Zyppy Plasmid Miniprep Kit, Zymo Research, Irvine, CA) and PCR of the gRNA sequence was performed using custom barcoded PCR primers (Supplemental Table S2 ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Biochemistry 2020Quote: ... Reactions were column-purified (Zymo research) and resuspended in nuclease-free water.
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bioRxiv - Biochemistry 2020Quote: ... Products of the first-round PCR were pooled and purified by a DNA clean and concentrator kit (Zymo) and diluted to 2 ng/μL ...
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bioRxiv - Microbiology 2020Quote: The double stranded deoxyribonucleic acids (dsDNA) was extracted from the duplicate sludge samples using ZR soil microbe DNA kit (Zymo Research, CA., USA), according to manufacturers instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Bisulfite converted DNA was prepared according using the EZ DNA Methylation-Lightning Kit (Zymo D5030). Library construction was performed using TruSeq DNA Methylation Kit (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... bisulfite converted DNA was prepared using the EZ DNA Methylation-Lightning Kit (Zymo D5030). PCR was performed with 10-20ng of bisulfite converted material for each locus of interest (see Table S1) ...
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bioRxiv - Bioengineering 2020Quote: ... and the DNA was obtained via Zymoclean Gel DNA Recovery Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... All pre-ligation gel extractions utilized Zymoclean Gel DNA Recovery Kits (Zymo Research). The β-lactamase plasmid was prepared for ligation in a similar process ...
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bioRxiv - Bioengineering 2020Quote: ... All pre-ligation gel extractions used Zymoclean Gel DNA Recovery Kits (Zymo Research). The GFP11 plasmid was prepared for ligation in a similar process ...
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bioRxiv - Bioengineering 2020Quote: ... All pre-ligation gel extractions used Zymoclean Gel DNA Recovery Kits (Zymo Research). The His6 production plasmid was prepared for ligation in a similar process ...
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bioRxiv - Bioengineering 2020Quote: ... These fragments were then purified using the DNA Clean and Concentrator Kit-5 (Zymo Research). The purified amplicons were then fused by performing a Golden Gate assembly reaction (Weber et al. ...
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bioRxiv - Bioengineering 2020Quote: ... using Direct-zol™ RNA MicroPrep Kit (Zymo Research, Freiburg) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Purification of mRNA was performed using the RNA Clean and Concentrator Kit (Zymo Research R1017). gRNA was stored at −80°C immediately after measuring concentration and diluting to 130 μM.
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using a Zymo DNA Clean and Concentrator Kit (Zymo Research D4014). Sequences for these primers and thermocycling conditions are given in Figure S7)
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated using the commercially available Quick-RNA kit (Zymo Research, Irvine, CA). RNA concentration and purity were assessed using a Nanodrop machine via absorbance at 280 nm ...
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bioRxiv - Bioengineering 2020Quote: ... The cultures were incubated in 96-well blocks (Zymo) at 30 °C with agitation for a total of 8 hours ...
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bioRxiv - Bioengineering 2020Quote: ... 10 µl of Zymolyase (Zymo Research E1004) or 50 µl Lyticase from Arthrobacter luteus (Sigma L4025-25KU ...
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bioRxiv - Bioengineering 2020Quote: ... Sequence verified and purified plasmids were transformed into yeast strain W303α using the Frozen-EZ Yeast Transformation II Kit (Zymo research) according to the manufacturer’s instructions and plated on YNB-URA dropout plates ...