Labshake search
Citations for Zymo Research :
8451 - 8500 of 9639 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: DNA extraction from clonal iPSC lines cultured in 96-well plates was performed using the Quick-DNA 96 Kit (Zymo Research). Briefly ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... Endotoxin-free plasmid library DNA was prepared using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research). To clone the library of gRNA pairs ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA from BMDM cell lines or tissues was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... bisulfite conversion was conducted according to manufacturer protocol with the EZ DNA Methylation-Gold Kit (Zymo Research) using 5 µL of in vitro methylated plasmid DNA or 1.5 µg genomic DNA measured with the Qubit dsDNA BR Assay (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... RNA extraction was performed and contaminating DNA was removed with DNase I (#E1010, Zymo Research) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 1 μg of genomic DNA was digested by 10U of DNA Degradase Plus (Zymo Research) overnight at 37°C ...
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bioRxiv - Genomics 2020Quote: ... The pooled cDNA was purified by Zymo DNA Clean & Concentrator Kit (D4013) ...
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bioRxiv - Zoology 2020Quote: DNA was extracted using the ZR Genomic DNA II kit (Zymo Research) and amplified at 12 loci microsatellite (Cliµd01 ...
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Microbiology 2020Quote: ... All genomic DNA used for screening PCR was isolated using the Quick-DNA miniprep kit (Zymo Research). The resulting knockout strain was designated Variovorax CL14ΔHS33.
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bioRxiv - Microbiology 2020Quote: ... pJMC158 DNA was isolated from a clone using the ZR Plasmid Miniprep Classic Kit (Zymo Research), sequence confirmed ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted with TRI Reagent (Zymo Research, R2050-1-200). RNA was then isolated using the Zymo Direct-zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was then isolated using the Zymo Direct-zol RNA Miniprep Kit (Zymo Research, catalog number R2053) and reverse-transcribed using Bio-Rad iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... We purified genomic DNA from the pooled cells using a Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). We performed whole genome sequencing of the two pools essentially as described (Lee et al. ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were resuspended in 500 µL water and transferred into ZR Bashing Bead Tubes (Zymo Research, CA). The ZR Bashing tubes were centrifuged for 1 minute at 8000g to discard the water ...
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were lysed with the addition of 100 μL of DNA binding buffer (Zymo Research), and tagmented DNA was purified with a 1.5:1 ratio of AMPure XP beads (Beckman ...
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bioRxiv - Genomics 2020Quote: Total RNA from testicular tissue samples of the four bulls was extracted using Direct-zol RNA Mini Prep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Zymoclean Gel RNA recovery kit (Zymo research) was used to recover and clean the target RNA ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using the RNA Clean and Purification kit (Zymo research) with DNAse step following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... followed by RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research). Reverse transcription of 900ng of template RNA into cDNA was done using random-hexamer primers with the Revertaid first strand cDNA synthesis kit (Thermo Scientific) ...
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bioRxiv - Genomics 2020Quote: ... and total RNA was isolated using the Diret-zol RNA mini-prep kit (Zymo Research R2051). Sequencing libraries were prepared using the QuantSeq 3’-mRNA Seq Library Prep Kit for Illumina (Lexogen ...
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bioRxiv - Microbiology 2020Quote: ... and RNA was purified according to the manufacturer’s protocol utilizing the Direct-zol RNA Miniprep Plus Kit (Zymo Research). Purified RNA was subsequently used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kits (Thermo-Fischer) ...
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bioRxiv - Microbiology 2020Quote: Media was cleared from cells and Trizol reagent (Zymo Research) directly added ...
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bioRxiv - Physiology 2020Quote: ... RNA was isolated and DNase treated (Zymo Research), then used for cDNA library synthesis (NEB E6560) ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested in Trizol at the specified time points and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1-hour DNase treatment ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate) and digested with EcoRI and BamHI alongside digestion of the base plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate). Successful Gibson Assembly reactions occurred using a 7:1 molar ratio of insert:backbone.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Following desalting using an Oligo Clean & Concentrator (Zymo Research), the polymerase reaction products were subjected to MALDI-TOF-MS (Bruker ultrafleXtreme ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA purification was performed using RNA Clean and Concentrator (Zymo Research) with the optional DNase I treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were purified using the Quick-RNA Mini Prep kit (Zymo Research). Quantitative PCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... and RNA was isolated using Direct-zol 96 (Zymo Research). RNA-seq libraries were prepared from 500 ng of RNA using Quant-seq 3’ FWD (Lexogen ...
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bioRxiv - Plant Biology 2020Quote: ... reinhardtii RNA for qRT-PCR analyses was isolated by using Quick-RNA™MiniprepKit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Dnase digested RNA was further purified using an RNA Clean & Concentrator™-25 (ZYMO RESEARCH) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2020Quote: ... to remove the template and subsequently cleaned up with a DNA cleanup kit (Zymo Research) before Gibson assembly ...
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bioRxiv - Cancer Biology 2020Quote: ... cfDNA was isolated from 0.5 - 10 ml of plasma using the Quick-cfDNA Serum & Plasma Kit centrifugation protocol according to the manufacturer’s protocol (Zymo Research). cfDNA was eluted twice in the provided elution buffer or in MilliQ and subsequently stored at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... was bisulfite-converted by using the EZ DNA Methylation kit (Zymo Research). PCR products were gel-purified (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 80 uM of “a-factor” (Zymo Research catalog # Y1004-500) was added to inhibit the growth of switched α cells before HO induction ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted using a Directzol RNA Extraction Kit (Zymo Research, http://www.zymoresearch.com). qPCR was performed as previously described by Waite and Dardick (2018) ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... A total of 150 ng of the ZR small RNA ladder (Zymo Research) and 120 pmol of a homemade mix of synthetic RNA (MX with 31,29 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was purified with RNA Clean and Concentrator 25 columns (Zymo Research). Ten micrograms of each sample was loaded onto a 10% polyacrylamide gel containing 8 M urea ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of DNA was bisulfite-converted using EZ DNA Methylation-Direct Kit (Zymo Research) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research) and Mlh1−/− tissues were used as Mlh1+/+ and Mlh1−/− allele controls ...
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bioRxiv - Synthetic Biology 2020Quote: Strain Xjb(DE3) was obtained from Zymo Research (Irvine ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: ... total RNA was collected from adherent cells using the Quick RNA Miniprep Kit (Zymo Research) following manufacturer instructions ...