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Citations for Zymo Research :
751 - 798 of 798 citations for Dendritic Cell Specific Transmembrane Protein DCSTAMP Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: ... we evaluated different commercial DNA extraction kits and procedural modifications by extracting Lehman Caves samples as well as “spike in” experiments in which cells from either an Acidithiobacillus thiooxidans culture or a microbial community standard (ZymoBIOMICS Microbial Community Standard, Zymo Research Corp.) were added to low biomass gypsum collected from the Frasassi Caves.
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: RNA from HEK Flp-In™ T-REx™ 293 and A549 cells was extracted using Zymo III columns (Zymo, Freiburg, Germany). Reverse transcription was performed using random hexamer primers (IDT ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to purify genomic DNA from the cells followed by bisulfite conversion using 50 ng DNA along with the EZ DNA Methylation- Gold kit (Zymo Research D5005). 10 ng of bisulfite-converted DNA was used to generate the whole genome bisulfite sequencing (WGBS ...
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bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 250 μl of this cell suspension was added to 250 μl of lysis buffer in ZR BashingBead™ lysis tubes (Zymo Research) containing 0.1 mm and 0.5 mm beads ...
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bioRxiv - Microbiology 2022Quote: ... gondii infected-PC12 cells that were harvested on day five post-infection using the Direct-zol RNA MiniPrep Plus kit (Zymo Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from HBEC controls and various HBEC derivative cells (three different clones for each group) using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). DNA samples were submitted to the Van Andel Institute’s Genomics Core (Controls ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Paleontology 2024Quote: ... qPCR was used to calculate the absolute number of bacterial cells in each sample by targeting the 16S rRNA gene using a Femto Bacterial DNA Quantification Kit (Zymo Research International). DNA sequencing was also carried out on a negative control (no contamination was found) ...
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bioRxiv - Neuroscience 2024Quote: RNA from biological duplicates for each condition (more than 30,000 cells for each sample) was extracted using the Quick-RNA Miniprep Kit (Zymo; Cat. No. R1055). Libraries were prepared from total RNA (15 ng per sample ...
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted and DNAseI-treated from DAC-treated AF2 cells using the ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003). This RNA was converted to cDNA using SuperScript III First-Strand Synthesis System (Invitrogen Cat# 18080051 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from AsPC1 or OV90 cells transfected with 100 nM siSMNDC1 or siCON for 48 h was extracted using Quick-RNA MiniPrep (R1055, Zymo Research, Irvine, CA). Three biological repeats were performed ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and viral RNA in the cell supernatants were extracted by using Direct-zol RNA MiniPrep kit (Zymo research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted from the resuspended pellet using the Zymo Quick-DNA Microprep Kit (cell suspensions protocol) per manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The DNA was quantified using the dsDNA kit on a Qubit 2.0 (Thermo Fisher ...
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bioRxiv - Physiology 2022Quote: HepG2 cell samples were collected into QIAzol Lysis Reagent and total RNA was extracted using Direct-zol RNA Miniprep (Zymo Research, CA, USA). Liver samples were first homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA purified from treated cells was first subjected to bisulphite conversion using the EZ-96 DNA Methylation-Direct MagPrep Kit (Zymo Research, U.S.; #D5044) to convert any unmodified cytosine residues to uracil ...
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bioRxiv - Bioengineering 2023Quote: ... and total genomic DNA was extracted from algal cell pellets (∼50-100 mg) with a Zymo Quick-DNA fungal/bacterial extraction kit (Zymo Research group, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: For DNA extraction cells were collected via filtration and filters transferred to 5 ml cryo vials containing 1 ml DNA/RNA shield (Zymo Research, Irvine, CA) as preservative ...
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bioRxiv - Genetics 2024Quote: ... A sample of sporulation culture with approximately 108 cells/mL was mixed 9:1 with a 5U/µL solution of Zymolyase (Zymo Research, cat. E1004) to a final zymolyase concentration of 0.5U/µL and incubated at 30°C for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from the isolated meiocytes (WT, clsy3, and clsy4) or tapetal cells using Direct-zol RNA Kit (Zymo Research, cat. no. R2061). Sequencing libraries were constructed using the RealSeq-Biofluids NGS Library Preparation Kit for miRNAs and small RNAs (BioCat ...
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bioRxiv - Microbiology 2022Quote: ... Total DNA from the infected and uninfected control cells was isolated using a Quick-DNA miniprep kit according to the manufacturer-recommended protocol (Zymo Research, Irvine, CA, USA). To measure HIV-1 PVI ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... were then homogenised in 200 µl nuclease-free water using a tube pestle and host cells lysed by addition of 1 ml of host depletion solution (Zymo Research, Irvine, CA, USA) with a 15 min incubation at room temperature with end over end mixing ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was extracted from the iPSC colonies after single cell sorting and clonal expansion by using the Quick-DNA™ Miniprep Kit (Zymo Research 11-317A) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from 1 × 105 cells using the Quick-RNA MiniPrep kit with Zymo-Spin IIICG columns (Zymo Research, Irvine, CA USA) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from each of the two technical replicates of sorted CFSElow CD4 T cells using Quick-RNA Microprep kit (Zymo Research, Irvine, CA, USA). Without measuring the resulted RNA concentration ...
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bioRxiv - Immunology 2021Quote: ... Single viable CFSElow CD3+ CD8− CD4+ T cells were sorted into 96-well PCR plates containing DNA/RNA Shield (Zymo Research, Irvine, CA, USA) using FACSAria II and Diva Software (BD Biosciences ...
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bioRxiv - Pathology 2021Quote: Total RNA was obtained from choroidal EC cultures and frozen RPE cells using an RNA purification kit (Direct-zol RNA MiniPrep; Zymo Research, Irvine, CA, USA), as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: DNA from each pool of 5-HT+ or 5-HT-cells was isolated and bisulfite-converted using the EZ DNA Methylation-Direct Kit (Zymo Research, Irvine, CA, USA) with the following adaptations ...
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bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA plasmid present within the yeast cells was extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Purified yeast-extracted DNA plasmid was then used to transform competent E ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA constructs encoding single macrocyclic peptide ligand were verified by Sanger sequencing and further used to transform new competent EBY100 cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... JRY114 was generated by plating 20 µl JRY101 cells grown overnight in rich non- selective media (YEPD) onto synthetic complete media supplemented with 5-FOA (Zymo Research, cat. No. F9001-5) (Boeke et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA of bacterial samples as well as eukaryote cells was purified using the Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, California, US) using the recommendations of the manufacturer.
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bioRxiv - Physiology 2022Quote: ... DNA was extracted from packed red blood cells (PRBCs) and frozen tissue samples (liver, gonad) using a commercially available DNA extraction kit (Zymo quick-DNA miniprep plus kit) as described previously ...
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bioRxiv - Genomics 2022Quote: ... total RNA was extracted from peripheral white blood cells using the Zymo Research Direct-zol™ DNA/RNA Miniprep kit (Zymo Research Corporation, Irvine, CA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... A total of 500 ng of genomic DNA from cells in co-cultures were bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA). The Infinium HD Methylation Assay (bisulfite modification ...