Labshake search
Citations for Zymo Research :
651 - 700 of 798 citations for Dendritic Cell Specific Transmembrane Protein DCSTAMP Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The assembled constructs were transformed into C122-metYp1p2-metY(CUA) cells made competent via Mix & Go (Zymo Research, cat. #T3007). PLB was added to a total of 1 mL ...
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bioRxiv - Neuroscience 2024Quote: The total RNA of SH-SY5Y or differentiated SH-SY5Y cells was extracted following the manufacturer’s instruction using the Quick-DNA/RNA MiniPrep Plus kit (ZYMO RESEARCH). 250 ng of total RNA was used for the reverse transcription of cDNA using TaqMan Reverse Transcription Reagents (applied biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then arrested in G1 using a final concentration of 4.0 µg/mL alpha-factor mating pheromone (Zymo Research) and incubated at 30°C with shaking until the vast majority of cells in the population exhibited the ‘shmoo’ phenotype (2.5 hours) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sorted cells were directly collected in lysis buffer for total RNA extraction using the Quick-RNA Micro-Prep kit (ZYMO). CD11chigh microglia were collected as cells with Venus fluorescence higher than the median Venus fluorescence of all CD11c+ microglia 18
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bioRxiv - Neuroscience 2024Quote: ... Treated and untreated control cells were collected with TRIzol reagent and purified using Direct-zol RNA microprep (Zymo Research #R2061). Sequencing libraries were generated from mRNA using a Oxford Nanopore cDNA-PCR v14 multiplex kit with the standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Microbiology 2024Quote: Total RNA was purified from the cell lysate using TRIzol-LS and a Direct-zol RNA Microprep kit (Zymo Research). RNA-Seq libraries were prepared with TruSeq Stranded Total RNA Library Prep Gold (Illumina) ...
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bioRxiv - Systems Biology 2024Quote: ... a commercial 1:1 mix of Imtechella halotolerans and Allobacillus halotolerans was added (2 × 107 cells each per 20 µL suspension; Zymo product numbers LMG 26483 and LMG 24826 ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was then harvested from cells 24 h post-transfection using the Direct-zol RNA Miniprep kits (Zymo Research). To ensure rigor and reproducibility ...
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bioRxiv - Bioengineering 2024Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Genomics 2024Quote: ... Cultures were centrifuged and each cell pellet was re-suspended in 100-500µL of DNA/RNA Shield (Zymo Research; R1100). Samples were stored at -80°C prior to combining the cells according to the abundances summarized in Supplementary Table 2.
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... reporter assay was carried out as described above in presence of Nsp1 or Nsp2 followed by harvesting of cells in Trizol (Zymo Research). The RNA was isolated using Direct-zol RNA miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from the sorted or the control cells either using the Quick-DNA Mini Kit or the Midi Plus Kit (Zymo Research). The integrated LentiCRISPRv2 vector sgRNA sequences were amplified through three rounds of PCR using the Taq DNA Polymerase S (high specificity ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA from BMDM cell lines or tissues was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Immunology 2021Quote: RNA extraction of whole lung pieces or purified cells was performed using the Quick-RNA Miniprep or Quick-RNA Microprep (Zymo Research), respectively ...
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bioRxiv - Cell Biology 2021Quote: mIMCD3 wild type and Jade mutant cells were washed with PBS and RNA extraction was performed with the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer’s instructions including a DNase1 treatment step ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: Dim and lit cells were sorted into 500 µl TriZol and RNA was extracted with DirectZol RNA purification kit (Zymo Research) with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from cultured cells using BIO TRI RNA reagent (Bio-lab) and Direct-zol RNA MiniPrep kit (Zymo research), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... The cells and medium were then collected and subjected to RNA extraction using the Direct-zol RNA MiniPrep kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Experiments to determine whether CxNV1 has DNA forms were initiated by extracting RNA from CT cells using Direct-Zol RNA MicroPrep (Zymo Research), and separately ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The remaining cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from cultured NSC-34 cells or primary motor neurons was isolated using DirectZole RNA Purification Kit (Zymo Research, R2052), and reverse transcribed using qScript cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus preparation or supernatants of virus-infected cells using a viral RNA extraction kit (Quick-RNA Viral Kit, R1035, Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were sorted by FACS as described above and RNA were extracted by using Quick- RNA Microprep Kit (Zymo Research, R1050) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The extracted DNA (100-1000 ng) from anal cells was bisulfite conversion by using EZ kit Gold Bisulfite Conversion Kit (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from cells following CTS or control treatments using the ZR-Duet™ DNA/RNA MiniPrep kit (Zymo D7001). RNA concentration and quality were measured using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...