Labshake search
Citations for Zymo Research :
6251 - 6300 of 6536 citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-S-UTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research, R1013) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research, R1058) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... These samples were used as the starting material for an RNA extraction using the Quick-RNA Viral Kit (Zymo, Cat no: R1034). The final elution was collected in 15 μl of nuclease-free water.
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bioRxiv - Cell Biology 2023Quote: ... was extracted from podocytes of Ercc1/Nphs2.Cre/mTmG mice using Direct-zol™ RNA MiniPrep Kit (cat. no. R2052, Zymo Research). Isolation of glomeruli ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Plant Biology 2023Quote: ... The adapter-ligated DNA molecules were purified using 1× Agencourt AMPure XP magnetic beads and were subjected to bisulfite conversion using ZYMO EZ DNA Methylation-Gold Kit (Zymo, Cat. #D5005). Libraries were then amplified by PCR using 20 μL of bisulfite-converted DNA molecules ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from three biological replicates of vehicle-treated (Veh) and 48 h-TTX-treated (TTX) neurons (6 samples in total) were isolated using Quick-RNA Microprep kit (Zymo Research, R1051) according to the instructions of manufacturer ...
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bioRxiv - Systems Biology 2023Quote: ... The aqueous phase was transferred to another tube by pipette and cleaned up using a Zymo cleanup and concentrator kit (Zymo Research D4013), then subject to size selection using SPRI-select beads (Beckman Coulter B23317 ...
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bioRxiv - Microbiology 2023Quote: ... DNA bands at ∼380 bp were excised and purified utilizing the Zymo Gel DNA Recovery Kit (Zymo Research, Irvine, CA, United States). Purified PCR products were equimolar pooled for a final library concentration of 11 pmol/l ...
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bioRxiv - Cell Biology 2023Quote: ... The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060). All RNA samples were initially amplified using the Ovation RNA-Seq System v2 kit (Tecan Genomics) ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 vRNA was isolated from BAL supernatant (200 µL) and mucosal swabs (nasal, pharyngeal, and rectal) using the Zymo Quick-RNA Viral Kit (Zymo Research, USA) as previously described [27,29] ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from the livers or adipose tissue using Direct-zol RNA Microprep Kits (R2061, Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA was then isolated from the membrane using phenol-chloroform and concentrated with the RNA Clean and Concentrator kit (Zymo Research, R1013), followed by reverse transcription using the RT Oligo primer (Supplementary Data 12 and SuperScript III ...
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bioRxiv - Plant Biology 2023Quote: ... and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 using Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Genomics 2023Quote: ... and dissected tissues (head, abdomen, gonads) from adult unparasitised males and females using a Direct-zol RNA Microprep kit (Zymo Research, USA), including DNAse treatment following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA, United States). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ligation products were cleaned using AMPure XP Beads (Fisher, Cat. #NC9933872) before processing for bisulfite conversion using the Zymo EZ DNA Methylation Kit (Zymo, Cat. #D5001), and by amplifying the bisulfite converted products over 55 cycles of 95°C for 30 seconds followed by 55°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from line with Bon GLKD driven by MT-Gal4 (and control flies that express an shRNA against the white gene) was depleted of ribosomal RNA with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... ∼15 ng of genomic DNA from each sample was first spiked in with in vitro methylated lambda phage genomic DNA (0.2%) as controls and was then subjected to bisulfite conversion (EZ DNA Methylation-Direct Kit, Zymo Research Cat# D5020). Half of the bisulfite converted DNA was used for low input WGBS analysis (for 5mC+5hmC profiling) ...
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bioRxiv - Genomics 2023Quote: 10 ng of gDNA isolated from GFP+/DAPI-FACS-sorted cell populations was first spiked with lambda phage controls and was bisulfite-converted with the Zymo EZ DNA Methylation Direct Kit (Zymo Research, D5020) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 IVO blastocysts or 80 8-cell embryos were digested and bisulfite converted according to the manufacturer’s instruction of EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). Bisulfite-specific primers were designed with the online Methyl Primer Express ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... DNA of 86 ovarian cancer patients was isolated from paraffin-embedded normal tissue with Quick-DNA FFPE Miniprep kit (Zymo Research, USA); other DNA samples were isolated from blood using PureLink Genomic DNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research, #D4060). The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB ...
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bioRxiv - Paleontology 2024Quote: ... qPCR was used to calculate the absolute number of bacterial cells in each sample by targeting the 16S rRNA gene using a Femto Bacterial DNA Quantification Kit (Zymo Research International). DNA sequencing was also carried out on a negative control (no contamination was found) ...
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bioRxiv - Molecular Biology 2024Quote: The RNA was extracted and purified using the Directzol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research; Cat# R2052) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: RNA from biological duplicates for each condition (more than 30,000 cells for each sample) was extracted using the Quick-RNA Miniprep Kit (Zymo; Cat. No. R1055). Libraries were prepared from total RNA (15 ng per sample ...
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from thallus fragments using the ZR Fungal/Bacterial DNA Miniprep Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Five hundred nanograms of each DNA sample and control DNA were bisulfite-treated (BST) using the EZ DNA Methylation-Lightning Kit (Zymo Research, # D5030) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm successful gene insertion and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm presence of desired mutation and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Immunology 2024Quote: ... Libraries were generated by the Emory Integrated Genomics Core (EIGC) as follows: RNA was isolated using the Quick-RNA MicroPrep kit (Zymo,11-328M). 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara ...