Labshake search
Citations for Zymo Research :
6101 - 6150 of 6536 citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and total RNA was isolated by either phase separation with chloroform and isopropanol or using the Zymo Direct-zol RNA Miniprep kit (Zymo Cat #R2050). One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200 ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA). Small RNAs were prepared by GenXPro (GenXPro GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA).
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Genetics 2020Quote: ... The bisulfite conversion was performed on 100 ng of sperm DNA using the EZ DNA Methylation-Gold kit (Zymo Research, Freiburg, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... A total of 500 ng of genomic DNA was bisulfite-converted (BSC) using manufacturer-recommended EZ DNAm kits (Zymo Research; Irvine, California). After conversion ...
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bioRxiv - Genetics 2020Quote: ... and genomic DNA (gDNA) of surviving cells was isolated using a genomic DNA cleanup kit according to manufacturer instructions (Zymo Research, D4065). For Illumina sequencing and screening analysis ...
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bioRxiv - Genetics 2021Quote: ... The adaptor-ligated DNA samples were subjected to bisulfite conversion by the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA, USA) and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from PPN was extracted using the TransZol reagent (TransGen Biotech, China) in combination with the Direct-Zol RNA mini prep kit (Zymo Research, USA). cDNA was produced from 500 ng of total RNA with EasyScript Reverse Transcriptase (TransGen Biotech Cat #AE101 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA from ∼106 parental or sorted CEM/R5 cells or CD4 lymphocytes was purified using Quick-gDNA MiniPrep Kit (Zymo Research, USA). The CXCR4 target regions were PCR-amplified using 200 ng of genomic DNA ...
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated using TRIzol and the Direct-zol™ RNA MiniPrep Kit with two times of in-column DNase I treatment according to the manufacturer’s instructions (Zymo Research, R2053). RNA purity and concentration were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from the input library and from three biological replicates of phage exposed and unexposed samples using a ZymoBIOMICS™ DNA Miniprep Kit (Zymo Research), following the manufacturers protocol.
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bioRxiv - Microbiology 2019Quote: ... viral RNA from fecal pellets was extracted using the Quick-RNA Viral 96 Kits according to manufacturer’s protocol (Zymo Research, Irvine, CA). Tissue RNA extraction was performed using TRIzol (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified by gel extraction from a 1.5 % low-melt agarose gel using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Samples were quantified using the Qubit dsDNA HS assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Nucleic acid samples were extracted directly from oro-pharyngeal swab samples using a Direct-zol™ RNA MiniPrep Plus extraction kit (Zymo Research) and in full compliance to the manufacturers’ recommendations ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... DNA-digestion and RNA-extraction was performed using the Zymo-Spin IC Column from Direct-zol RNA MicroPrep kit (Zymo Research, R2060) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Bisulfite Conversion and DNA Yield Bisulfite conversion of extracted cellular or tissue DNA was performed using the DNA Methylation-Lightning™ Kit (Zymo Research) according to manufacturer’s instructions and eluted into 40 µl DNA Elution buffer (Zymo Research) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from yeast cells grown into SD-Trp-Leu to mid-log phase was isolated with YeaStar RNA Kit (Zymo Research R1002). RNA was treated with 0.02 units/µl DNase I (NEB M0303 ...
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bioRxiv - Cell Biology 2021Quote: ... was extracted from whole blood via cardiac puncture from 6-8-week-old Fzd2tm1Eem (homozygous flox or homozygous global mutants created by crossing with CMV-cre mice) or Fzd2tm1Vari (homozygous flox) animals using Quick-DNA™ Miniprep Plus Kit (Zymo Research). Illumina libraries were constructed with average insert sizes of ∼800 bp and sequenced on an Illumina NovaSeq6000 using paired-end 150bp reads ...
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bioRxiv - Cell Biology 2020Quote: ... mixed 1:1 with 100% ethanol and the mix was transferred to a column of the Direct-zol RNA MiniPrep Plus Kit (Zymo Research, #R2070). The following purification steps were done according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Fungal DNA from the cultures was extracted using a ZymoBIOMICS® Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the total RNA was extracted from the clear aqueous phase of the solution using Direct-zol™ RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Sodium bisulfite conversion of 500 ng of genomic DNA from each subject was performed using the EZ DNA Methylation-Gold™ Kit (Zymo, USA). DNA methylation levels at specific genomic sites were investigated by targeted deep bisulfite sequencing following the protocol by Moser et al ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mixture was cleaned and concentrated to 10 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). 1 μl of the codon randomised mRFP library was transformed into electrocompetent DH10B cells (20 μl competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The linear plasmid was excised from the gel and purified to 30 μl mQ using the Zymoclean™ Gel DNA Recovery Kit by Zymo (D4002). The codon random mRFP library (15 μl ...
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bioRxiv - Systems Biology 2022Quote: ... Total DNA extractions were performed for tea broth and pellicle samples using Quick-DNA Fecal/soil Microbe Miniprep kit (ZYMO Research Corp.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... insect legs were mechanically crushed and resuspended in 50 μl of sterile water and the genomic DNA (gDNA) was extracted using a Quick-DNA Universal kit (Zymo research, USA). 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega ...
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bioRxiv - Genomics 2022Quote: ... 500ng of high-quality genomic DNA was bisulfite converted using the Zymo EZ-96 DNA Methylation kit (Zymo Research, Irine, CA, USA). Bisulfite converted samples were then amplified ...
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bioRxiv - Molecular Biology 2022Quote: ... The dsDNA blocks were excised from the gel and purified to 20 μl mQ using the Zymoclean™ Gel DNA Recovery Kit (Zymo, D4002). 5 μl of the dsDNA was used to quantify the DNA concentration with the Qubit assay (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Molecular Biology 2022Quote: ... The fulllength assembled product was excised from the gel and purified to 44 μl mQ using the Zymoclean™ Gel DNA Recovery Kit by Zymo (D4002). 10 units of SapI (NEB ...
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bioRxiv - Microbiology 2022Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... epithelial sheets and hyphae pellets were grinded in liquid N2 and processed as described before (Amorim-Vaz et al., 2015a) combining two phase separations and DirectZol™ RNA MiniPrep kit (Zymo Research). DNase treatment was performed off-column using a DNA-free™ Kit (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the whole rosette leaves of 32 day-old control and drought-stressed plants using the Quick-RNA Miniprep Kit (Zymo-Research, USA). RNA quality and quantity were determined using a Nabi UV/Vis Nano Spectrophotometer (LTF Labortechnik ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated from tumor cell populations and unstained whole tumor single cell suspensions using the Quick-RNA™ Microprep Kit (Zymo R1050) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Tissue RNA extraction was performed using the Direct-zol RNA miniprep plus kit with DNAase treatment per package insert (Zymo, Irvine, California). SIV and CD4 primers for RT PCR and amplification conditions were as previously published [28] ...
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bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted from flash-frozen fetal liver (left lobe) samples using a Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA)) per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation of digested samples was performed using the Directzol RNA Miniprep kit with DNAse-I treatment to remove trace DNA before RNA elution (Zymo Research, R2050). RNA was quantified via Nanodrop spectrophotometry ...
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bioRxiv - Biochemistry 2022Quote: ... the LentiCRISPRv2 hygro plasmid was purified using a 0.7% agarose gel and a Zymoclean gel DNA recovery kit (Zymo Research cat # D4001). The prepared sgRNA oligo duplexes were then ligated into the purified LentiCRISPRv2 hygro plasmid ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA (250-500ng) was modified by sodium bisulfite reaction using the EZ DNA Methylation-Lightning kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions ...