Labshake search
Citations for Zymo Research :
5701 - 5750 of 6511 citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with sterile PBS and total RNA was isolated using the Direct-Zol Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was run on a 1% Agarose gel and the band at the appropriate size was excised and the DNA extracted using the Zymoclean Gel DNA Recovery kit (Zymo Research). Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The bands of interest were cut from the denaturing gels and purified using ZR small-RNA PAGE Recovery Kit (Zymo Research). The purified RNA samples were stored in nuclease free water at -20 °C.
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bioRxiv - Immunology 2022Quote: ... Cells were sorted into TRIzol (Thermo-Fisher) and RNA was isolated using a Direct-zol RNA-mini kit with RNA clean and concentrator columns (Zymo Research) and treated with DNase (Qiagen).
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... An equal volume of 100% ethanol (800uL) was added immediately and mixed well before proceeding to RNA isolation with the Direct-zol RNA Microprep Kit (Zymo Research). DNase treatment and RNA isolation proceeded according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were made from the Zymo mock community using the LoopSeq 16S long-read kit (referred to throughout as ‘Zymo-Loop’). This preparation was also followed for the ATCC-gut and ATCC-oral communities as well as the uterine lavage and cyanobacterial enrichment samples ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... DNA with a size of at least ~200 bases was excised and purified using a gel DNA recovery kit (Zymo Research) with elution in 50 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... The transposed DNA fragments were further amplified and barcoded (Buenrostro et al., 2013, 2015) and purified with a ChIP DNA Clean & Concentrator kit (Zymo, D5205). The fragments were run on 2% E-Gel™ EX agarose gels (Thermo Fisher scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Cell Biology 2021Quote: Cellular and tissue total RNA was extracted using TRI reagent and Direct-zol RNA MiniPrep Plus kit (Zymo Research, Irvine, CA). First-strand cDNA was synthesized using the SuperScriptTM IV VILOTM cDNA synthesis kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Microbiology 2020Quote: ... The presence of inserted sequences in generated vaccine virus stocks was confirmed by RNA extraction (Direct-zol RNA kit, Zymo Research) followed by RT-PCR (qScript XLT ...
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Genomics 2020Quote: Sodium bisulfite conversion: 200 ng of NA12878 DNA was converted using the EZ DNA Methylation-Gold Kit (ZYMO Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Reactions were quenched with 8 volumes of ethanol and the DNA was recovered by using the DNA Clean and Concentrator kit (Zymo Research). DNA samples were digested to nucleosides using the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Immunology 2021Quote: Overnight cultures of MACS sorted antibody-escaped and ACE2 preselected yeast populations were proceed to yeast plasmid extraction kit (Zymo Research). PCRs were performed to amplify the N26 barcode sequences as previously described13 ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Product bands of the correct size (∼400 bp) were gel-purified using the Zymoclean™ Gel DNA Recovery Kit (Zymo, D4008) and subsequently analyzed by capillary electrophoresis (Fragment analyzer ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then de-crosslinked by incubation at 94C for 20 mins and subsequently lysed and extracted for genomic DNA (gDNA) using the Quick-DNA/RNA Miniprep Kit (Zymo D7005T). gDNA samples of both samples were sent to Cellecta Inc ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Developmental Biology 2022Quote: ... Glycerol stocks of the plasmids that were confirmed correct by Sanger sequencing were grown and prepared for maxiprep plasmid isolation (Zyppy Plasmid Maxiprep Kit, Zymo 6431). Proper cloning was achieved for all gRNAs.
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bioRxiv - Evolutionary Biology 2022Quote: ... The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina was used for library preparation and was combined with EZ-96 DNA Methylation-Gold™ MagPrep (ZYMO) for bisulfite libraries ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from the brain and muscle tissue separately using a Quick-DNA™ MagBead Plus kit (Zymo Research, 2017) and quantified using a Qubit™ dsDNA HS Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and the total RNA was then extracted using the Direct-zol RNA MiniPrep Kit (R2052, Zymo Research) and quantified using NanoDrop 2000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was first extracted from pools (10 flies for each cage and time point) using the Quick-DNA Plus kit (Zymo D4068), which includes a proteinase K digestion to ensure unbiased sampling of diverse bacteria ...
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then placed on a magnet and the immunoprecipitated DNA samples in the supernatant along with the input samples were purified using ChIP Purification Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified from proteinase K treated samples using a DNA purification kit following the manufacturer instructions (Zymo Research, CA, USA) and was subsequently analyzed either by running a 2% low melting agarose (APEX ...
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bioRxiv - Microbiology 2022Quote: ... DNA and RNA were extracted using the Quick DNA/RNA mini prep plus kit with ZR BashingBeads (0.1 and 0.5mm; Zymo Research, Carlsbad, CA). For coral samples ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: ... gastrocnemius muscle RNA from Sham and SMC mice were isolated with the Direct-zol RNA Miniprep Plus kit (Zymo Cat#: R2070). RNA library construction and sequencing were performed by the High-Throughput Genomics Core at the Huntsman Cancer Institute ...
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bioRxiv - Microbiology 2019Quote: ... the 180 basepair-long amplicons were purified by using a Zymo Research DNA Clean and Concentrator™ Kit according to the manufacturer’s protocol (Zymo Research) and eluted in in 30 μl 1× Tris-EDTA (TE ...
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bioRxiv - Microbiology 2019Quote: ... and PCR products were extracted and purified from agarose gels using the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). The Zyppy Plasmid Miniprep Kit (Zymo Research ...
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bioRxiv - Microbiology 2019Quote: ... Digests were heat inactivated for 20 minutes at 80°C then column cleaned using the DNA Clean and Concentrator kit (Zymo Research). Dilute ligations (100-500 ng DNA ...
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bioRxiv - Developmental Biology 2019Quote: Sodium bisulfite modification of genomic DNA (500 ng) of WT and Etv5 KO mESCs was performed according to the instruction of EZ DNA Methylation-DirectTM Kit (Zymo Research). Methylation specific PCR for a fragment of Fgfr2 promoter was carried out as we previously described (Zhang et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 µL of this soma sample was then carried forward for RNA isolation using the Zymo QuickRNA MicroPrep kit (Zymo R1050). In parallel ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 ng of genomic DNA was digested with the appropriate restriction endonuclease and purified using Genomic DNA Clean & Concentrator kit (D4065-Zymo Research). 0.6 ng was used as template for qPCR analysis using PowerUp SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Two rounds of bisulfite conversion of the adapter-ligated DNA were performed using EZ-DNA methylation Gold Kit (D5005-Zymo Research), and used as template for three separate PCR reactions (eight cycles of amplification ...
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bioRxiv - Bioengineering 2019Quote: The frozen samples were subjected directly to DNA extraction with the ZymoBIOMICS™ DNA Miniprep Kit (Zymo Research, Irvine, CA, USA), using a PowerLyzer® 24 Bench Top Bead-Based Homogenizer (MO BIO Laboratories ...
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bioRxiv - Microbiology 2019Quote: DNA fragments < 700 bp in length were isolated from 9 μg of total genomic DNA using a Select-a-Size DNA Clean & Concentrator kit (Zymo Research) according to manufacturer’s recommendations ...