Labshake search
Citations for Zymo Research :
5551 - 5600 of 6511 citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA concentration was measured on a NanoDrop and 50µg RNA was purified using an RNA Clean & Concentrator-25 Kit (Zymo, R1017), with on-column DNaseI digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... RPFs were isolated using MicroSpin S-400 columns (Cytiva) and purified using the RNA Clean & Concentrator-25 kit (Zymo Research). After size-separation of RPFs using a 15% polyacrylamide TBE-urea gel (Coolaber Technology Co. ...
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bioRxiv - Cell Biology 2023Quote: ... An additional plate grown in parallel was reserved for total genomic DNA extraction (Quick-DNA Universal Kit (Zymo Research, CA)) ...
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bioRxiv - Genetics 2023Quote: ... 100μl of the gDNA solution was purified using the Zymo Genomic DNA Clean and Concentrator –10 kit (Zymo Research #ZD4010) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 1 ug of genomic DNA was bisulfite (BS)-converted using the EZ DNA Methylation Gold Kit (Zymo Research Cat# D5006) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... total RNA was extracted from fresh apical-out enteroids using Zymo Direct-Zol RNA miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted from isolated fly heads using the Zymo Direct-Zol RNA miniprep kit (Zymo Research, Irvine, CA). Extracted RNA was quantified on a Nanodrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The RNAs were further purified with phenol/chloroform/isoamyl alcohol mix (pH 6.6) followed by clean-up with RNA Clean & Concentrator Kit (Zymo research). The purified RNAs were reverse transcribed using SuperScript IV (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... and a pool of L3 larvae (A. caninum) using a Quick-DNAÔ HMW MagBead Kit (Zymo Research, Irvine, CA, USA) and modified Solid Tissue extraction protocol (Supplemental Table 1) ...
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bioRxiv - Physiology 2024Quote: ... RNA was extracted from Tibialis anterior (TA) muscles from each group using The Direct-zol RNA MiniPrep kit according to manufacturer’s instructions (Zymo Research). RNA quality for sequencing was determined using the high-sensitivity Agilent RNA ScreenTape Assay for 4200 Tape Station System (Agilent Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA for 16S rDNA amplicon next generational sequencing was isolated using the ZymoBIOMICSTM – 96 DNA Kit (Zymo Research, D4309). The 16S amplicon library was prepared in a 96-well format using dual-index barcodes [80] ...
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bioRxiv - Plant Biology 2024Quote: ... and the DNA contained therein was excised and extracted using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Each transcript extracted was then cloned into a pRACE cloning vector (Clontech Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 300 ng of DNA was treated with bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, D5005, USA) and amplified by PCR using Takara Premix Ex TaqTM Hot Start Version (Takara Research ...
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bioRxiv - Molecular Biology 2024Quote: ... Selected colonies will be transferred to liquid culture and then plasmid isolation will be performed with the ZymoPURE Plasmid Maxiprep Kit (#D4202, ZYMO).
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bioRxiv - Molecular Biology 2024Quote: Pellets were resuspended in 500µL TRIzol reagent and total RNA was extracted using a Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Microbiology 2024Quote: ... beads were removed by centrifugation (20,000 × g, 5 min) and total RNA was isolated using the Direct-zol RNA miniprep kit (Zymo Research) as directed by the manufacturer.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted by Direct-zol RNA Miniprep Plus kit according to the manufacturer’s instruction (Zymo, Catalog no. R2072) and then reverse-transcribed into complementary DNA using the iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from pelleted cells as described previously16 using a ZymoBiomics DNA Miniprep kit (Zymo Research; Irvine, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were then reverse-crosslinked overnight in elution buffer (1 % SDS, 0.1 M NaHCO3) and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated as described and RNA was extracted using the Zymo Quick-RNA MiniPrep Kit (Zymo Research, catalog R1055) following the kit instructions ...
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bioRxiv - Zoology 2024Quote: DNA from the homogenized termite guts was extracted using the Quick-DNA™ Fecal/Soil Microbe Microprep Kit (Zymo Research) following kit instructions ...
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bioRxiv - Zoology 2024Quote: ... The cDNA to be used as a template for PCR amplification was obtained from a mix of ten 1 to 25 days-old CSFB adults by RNA extraction using Quick tissue kit (Zymo) and reverse transcription using the LunaScript® RT SuperMix Kit ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from homogenized mouse skin punch biopsies using a Direct-zol RNA MiniPrep Plus kit (Zymo research, R2072). Bone marrow-derived macrophages were plated at 2×105 cells/mL and treated with PBS or 500 ng/mL PAM3CSK4 (Tocris Bioscience ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from cells sorted into high and low PER1 expression bins was extracted using the FFPE miniprep kit (Zymo). Genomic DNA purified using the FFPE miniprep kit was then cleaned using Axiprep Magnetic Beads (Axygen ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 80-100 mg of tissue with the Direct-Zol RNA extraction kit (Zymo Research, CA, USA) as per the manufacturer guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mg of genomic DNA was treated with sodium bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and lysed by vortexing with 0.5 mm zirconia glass beads before extracting RNA using Zymo Direct-zol kits (Zymo #R2072).
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bioRxiv - Systems Biology 2024Quote: ... and then each sample was applied to a spin column supplied in the RNA clean and concentrator kit (Zymo Research). Samples were centrifuged at 12,500 rcf for 30 seconds ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of sheared DNA was bisulphite-converted using the EZ DNA Methylation-Gold Kit (D5005; Zymo Research, Irvine, CA). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR bands were cut out from agarose gels and purified with Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA).
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bioRxiv - Molecular Biology 2024Quote: ... Products from PCR1 were run on a 2% agarose gel in TAE (Tris-acetate-EDTA) buffer and fragments were extracted using the Zymo Gel DNA recovery Kit (Zymo). Eluted products were pooled in equimolar amounts and were submitted to the UC Davis DNA Technologies and Expression Analysis Core lab for a second round of PCR (PCR2 ...
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bioRxiv - Microbiology 2024Quote: ... The product of this reaction was then run on a 0.8% agarose gel and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research) to yield the final Spike DNA fragment ...
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bioRxiv - Plant Biology 2020Quote: ... and RNA greater than 17 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1017). After checking digestion quality ...
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bioRxiv - Plant Biology 2020Quote: ... RNA less than 200 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1015). Next ...
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bioRxiv - Synthetic Biology 2021Quote: ... Bands of the expected size were cut out and DNA was extracted with a ZymoClean Gel DNA recovery kit (Zymo Research). For all cloning steps ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the RNA volume was brought up to 100 μL with nuclease-free water and was purified away from other reaction components by an RNA Clean & Concentrator kit (Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The completed PCR reactions were pooled and ethanol-precipitated prior to purification using a DNA Clean & Concentrator Kit (DCC-25, Zymo Research). The concentration of the labeled 197-bp PCR products was determined by UV absorbance ...
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bioRxiv - Cell Biology 2020Quote: ... and the RNA was extracted using the Direct-zol™ RNA purification kit following the manufacturer’s protocol for DNase I digestion (Zymo Research). cDNA was synthesized with Maxima Reverse Transcriptase (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2020Quote: ... The parasites stages were homogenized in TRIzol with DNA and total RNA was extracted using the Direct-Zol RNA MiniPrep Plus kit (Zymo Research) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Microbiology 2019Quote: ... The enriched circular DNA samples were then purified by DNA Clean & ConcentratorTM-5 kit according to manufacturer’s instructions (Zymo Research, USA). Elution was done with 10 µl of 10 mM Tris (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Genomics 2021Quote: ... Fragments between 400-700 bp were excised from the gel and recovered using the ZymoClean Gel DNA Recovery Kit (Zymo Research). The final libraries were quantified using the Qubit dsDNA BR Kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Duplicate PCR reactions of each sample were carried out and the amplified products were pooled using a DNA Clean and Concentrator kit (Zymo Research). The resulting amplified fragments were analyzed via Sanger sequencing using both forward and reverse primers ...