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Citations for Zymo Research :
501 - 550 of 564 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Developmental Biology 2024Quote: ... we used limited-cycle PCR to amplify the oligopools (Twist Biosciences) and cleaned up the PCR product using DNA oligo purification kits (Zymo Research, D4003). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... We then digested the template DNA using TurboDNase at a concentration of 0.1 U/μl in 200 μl and cleaned up the reaction using the RNA Clean & Concentrator-25 kit (Zymo Research). Finally ...
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bioRxiv - Genomics 2022Quote: ... The reaction is incubated in the thermocycler at 50 degrees for 30 minutes and 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were purified from the reaction using a RNA Clean & Concentrator(tm)-5 column (Zymo Research, Cat. n° R1013). The resulting RNA fragments were ligated to different adaptors (listed in Supplementary Table 1 ...
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bioRxiv - Genomics 2021Quote: ... The bisulfite conversion reaction was subsequently washed and desulfonated using reduced volumes in a 384-plate spin column (Zymo Research) essentially as described in (Luo et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction volume was increased to 100 μl with H2O and further purified using RNA Clean & Concentrator (Zymo Research, R1013) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA on ice with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP).
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bioRxiv - Microbiology 2022Quote: 74μL of ultrapure water was added to the remaining sample (to make up to 100μL total volume) and each reaction was then purified using the Zymo Genomic Clean and Concentrate kit (Zymo D4011). The DNA was eluted with 10mM Tris buffer heated to 63°C and incubated for a few minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were cleaned up with RNA Clean and Concentrator 100 columns (Zymo, referred to as “kit-purified” in text). Purity was assessed using a 6% Tris-Borate-Urea gel (Invitrogen).
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bioRxiv - Synthetic Biology 2023Quote: ... A maximum of 96 PCR reactions were pooled at a time and cleaned with Zymo-Spin II columns (Zymo Research). Pooled samples were sequenced with the NGSelect Amplicon sequencing service of Eurofins Genomics.
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bioRxiv - Microbiology 2024Quote: ... The product of this reaction was then run on a 0.8% agarose gel and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research) to yield the final Spike DNA fragment ...
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bioRxiv - Genomics 2022Quote: ... 10 μl of ligated product was added to 40 μl nuclease-free water before performing the column purification with RNA Clean & Concentrator™ (Zymo Research, R1016). After that ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of the PCR product that contained the specific modules were stored in Zymo DNA/RNA shield (Zymo Research Cat.No R1100-50) in the freezer at -20 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by RNA isolation according to the TRIzol product instructions or using a Direct-zol RNA MiniPrep Plus kit (ZYMO Research Corp, 2072). DNA contamination was removed from the TRIzol-isolated RNA via enzymatic digestion with 10 U of Turbo DNase (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Bioengineering 2023Quote: ... Undigested bands corresponding to mutant PCR products were gel extracted with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Cat. No./ID: D4007), then cloned into pJET vectors (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was extracted using hot acid phenol method followed by purification using Direct-zol RNA Miniprep Kit (Zymo Research). The RNA was treated with DNaseI ...
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bioRxiv - Microbiology 2023Quote: ... Total nucleic acids were extracted from the Sterivix filters using the Zymo Direct-zol DNA/RNA Miniprep kit (Zymo Research). Extracted DNA was quantified using a Qubit v3.0 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid extraction and purification were performed using the Quick-DNA/RNA™ Viral MagBead (Zymo Research, Irvine, California, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... S26) were subject to a dual nucleic acid isolation protocol using the DNA/RNA Mini Prep Plus kit from Zymo Research (Irvine ...
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bioRxiv - Biochemistry 2021Quote: ... the RNA volume was brought up to 100 μL with nuclease-free water and was purified away from other reaction components by an RNA Clean & Concentrator kit (Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The completed PCR reactions were pooled and ethanol-precipitated prior to purification using a DNA Clean & Concentrator Kit (DCC-25, Zymo Research). The concentration of the labeled 197-bp PCR products was determined by UV absorbance ...
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bioRxiv - Developmental Biology 2021Quote: ... The reactions were incubated for 30 min at 37°C and immediately purified using ZYMO RNA Clean & Concentrator (ZYMO RESEARCH, D7011). The purified RNA samples were eluted in 20 μl of nuclease free water ...
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bioRxiv - Genomics 2020Quote: ... 5 µl reactions were purified with the ZR-96 Oligo Clean & Concentrator and 15 µl reactions were purified with the Oligo Clean & Concentrator kit (Zymo Research) and eluted with probe resuspension buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared supernatant was then transferred to a fresh reaction tube and applied to spin-columns (RNA Clean & Concentrator-5 columns, Zymo-Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... The reaction was then cleaned up using Zymo ChIP DNA Clean & Concentrator following the manufacturers’ instructions (Zymo Research; Irvine, CA, USA). The resulting PCR reaction was then cloned into pGEM T-Vector system and selected for using the XGal/IPTG system (Promega ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary validation of genotyping reactions was performed as needed using the Quick-DNA Plant/Seed Miniprep kit (Zymo Research, cat#D6020). Bulk Illumina and Nanopore data from Tpd1 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was digested by 2M NaOH and the reaction was neutralized with 1M Tris-HCl (pH 7.5) followed by column purification (Zymo research, R1016). The 5’adapter (for C2C12 libraries ...
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bioRxiv - Genomics 2023Quote: ... was transformed with 2 uL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research). Each cloned gRNA sequence-containing PB-CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio).
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Microbiology 2024Quote: ... The digested reaction mix was then purified prior to Gibson Assembly using a Zymo DNA clean and concentrator kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Multiple PCR reactions of each fragment were pooled and purified by gel purification using the ZymocleanTM Gel DNA Recovery Kit (ZYMO RESEARCH) and used for transformation.
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Developmental Biology 2024Quote: ... Hydrolysis was neutralized with 1M acetic acid before purifying the libraries using ssDNA/RNA Clean & ConcentratorTM (Zymo Research, 50-444-498).
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bioRxiv - Molecular Biology 2021Quote: ... templates were run on a 1% agarose gel to confirm cutting and the reactions were purified with Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). 1ug of linearized template was used in a 100uL T7 RNA Polymerase (purified in house ...
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bioRxiv - Molecular Biology 2020Quote: ... Immediately following digestion the reaction was stopped in the binding buffer from a Zymo ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205) and purified following the protocol in the kit ...