Labshake search
Citations for Zymo Research :
5251 - 5300 of 6550 citations for Rat LIM Domain Kinase 2 LIMK2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted following the manufacturer’s recommendations of the magnetic bead based Quick-DNA MagBead Plus Kit (Zymo Research). The DNA concentration of each sample was measured using a Qubit fluorometer (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA from the DRG and SGC cultures was extracted using the Direct-zol RNA Miniprep Kit (Zymo Research), and 0.5–1 μg RNAs were reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... and stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were placed on ice and then purified using Zymo DNA clean and concentrator kit -5 (Zymo #D4004) following cDNA protocol ...
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bioRxiv - Cancer Biology 2023Quote: RNAs from respective cell lines were isolated and purified via the Zymo Quick-RNA microprep kit (Zymo Research, #R1058). 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng genomic DNA (gDNA) was used for bisulfite conversion using the EZ DNA methylation kit (Zymo Research, D5001). The bisulfited gDNA was denatured and neutralized for amplification ...
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bioRxiv - Microbiology 2023Quote: ... and the RNA samples were extracted using TRIzol reagent and purified using Direct-zol RNA Purification Kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction and DNase treatment were performed using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Adaptor-ligated fragments were then bisulfite-treated using the EZ DNA Methylation-Lightning™ Kit (Zymo Research, Cat# D5459). Preparative-scale PCR (16 cycles ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted with Direct-zol RNA miniprep kit with on-column DNase I digestion (Zymo Research #R2053). 0.5 to 1 μg of total RNA was used for reverse-transcription with ReverTra Ace qPCR RT master mix (Toyobo #FSQ-101 ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was isolated either via chloroform extraction or using a Direct-zol MiniPrep or MicroPrep kit (Zymo Research).
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bioRxiv - Immunology 2023Quote: ... cells and tissue were harvested in TRIzol and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1 h DNase treatment ...
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bioRxiv - Bioengineering 2024Quote: ... plasmid Homology Directed Repair Templates were produced by bacterial culture and standard plasmid preparation (Zymo Midiprep and Maxiprep kits) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cell samples were lysed with Trizol (Life-science) and RNA purification was done with Zymo kit (R1050, Zymo). RNA quantification was assessed with a Nano Drop 8000 Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Genomics 2023Quote: ... cleaned up either with phenol-chloroform and precipitated with ethanol or with RNA Clean & Concentrator -5 kit (Zymo Research). Two biological replicates were collected for each genotype ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was treated with DNase I and cleaned using an RNA Clean and Concentrator Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... These preparations were then transferred to Zymo Research RNA clean and concentrator-25 kit columns (Zymo Research, cat. R1018) and subjected to multiple wash and centrifugation steps as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bulk soil samples using Quick-DNA™ Fecal/Soil Microbe Miniprep Kit (Zymo Research). Quantity and purity of DNA isolated from bulk soil samples were assessed using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were cleaned and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... the reaction was stopped using RNA binding buffer from the RNA Clean & Concentrator Kit (Zymo Research, Irvine, CA, USA) plus ethanol following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Omega Bio-tek®) and subjected to DNase treatment and purification by the RNA Clean & Concentrator kit (Zymo Research) to yield high-quality RNA for library preparation ...
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bioRxiv - Genetics 2024Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
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bioRxiv - Cell Biology 2024Quote: ... Denmark) for 24 hours prior to total RNA extraction using Direct-zol™ RNA Miniprep kit (Zymo Research, USA). 100-150 islets in technical duplicates were used per condition ...
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bioRxiv - Cancer Biology 2024Quote: ... range: 10.52 ng - 22.9 ng) of cfDNA was bisulfite converted using the EZ DNA Methylation-Lightning Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA (3µg) was purified with RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA, USA, Ref. ZR1016), keeping only small RNAs fragments (17 - 200 nt ...
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bioRxiv - Immunology 2024Quote: ... PCR products were then identified by agarose gel method and purified via Zymoclean Gel DNA Recovery Kit (Zymo Research). Sanger sequencing of clones was performed by Quintara Biosciences to identify clones that displayed the proper genetic modification ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products of each group were pooled and purified using DNA Clean & Concentrator-25 kit (Zymo Research, #D4034), followed by next-generation sequencing (NGS ...
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bioRxiv - Microbiology 2024Quote: ... total DNA was extracted from approximately 1.0 × 104 neurons using the Quick-DNA™ Miniprep Plus Kit (Zymo Research). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix (ThermoFish Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were pooled and purified with a DNA Clean & Concentrator purification kit (Zymo Research, San Diego, CA, USA). The pooled amplicon library was sequenced on an Illumina MiSeq platform at the Idaho State University Molecular Research Core Facility (RRID:SCR_012598).
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bioRxiv - Developmental Biology 2024Quote: ... Polysome-bound RNA was retrieved from Dynabeads and DNase-treated using the Direct-zolTM RNA MicroPrep kit (Zymo Research).
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bioRxiv - Neuroscience 2024Quote: ... knockdown efficiency was calculated via qPCR: RNA was extracted with the Zymo Direct-Zol RNA microprep kit (#R2062, Zymo); cDNA synthesis was performed with the SuperScript IV First Strand Synthesis System using oligo-dT priming (#18091050 ...
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bioRxiv - Microbiology 2024Quote: ... Replicon DNA was isolated after another 6-hour growth using the ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research). Reporter replicon-transfected HEK293T cells were imaged 48 h after transfection on an EVOS M5000 Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted from a total of 100 mg of ground frozen shoot tissue with Direct-zol kit (Zymo Research) according to manufacturer’s protocol with in-column DNAse treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluates were then concentrated in a SpeedVac to ∼100 µL and purified with the DNA Clean and Concentrator Kit (Zymo). Libraries were sequenced on a HiSeq 2000 at the BioMicroCenter Core Facility at MIT with 50 nt read length.
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was extracted from no treatment and SHX-treated flash frozen cells using the Quick RNA extraction kit (Zymo) following the standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted using Direct-zol RNA MicroPrep kit according to manufacturer’s instructions (cat no. R2062, Zymo Research, Irvine CA). Purified RNA samples underwent fragment analysis at the MIT BioMicro Center ...
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bioRxiv - Cell Biology 2019Quote: RNA was typically isolated from confluent monolayers of cells seeded in 10-cm plates using the direct-zol RNA miniprep kit from Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cells lysed with Trizol using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2072). 1 µg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: IL2 and CD20 constructs were created through PCR amplification from cDNA and genomic DNA respectively.PCR products were purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) and cloned into the pEntryD-Topo vector ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from strains that had at least an IC50 shift of 1.5-fold was extracted using the YeaStar Genomic DNA kit (cat. No D2002, ZYMO Research).
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted from embryos and three-week old plants using Quick-DNA™ Micro prep Kit (Zymo D3020) according to the recommendations of the manufacturer.
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bioRxiv - Evolutionary Biology 2021Quote: ... bacterial colonies were selected and grown in overnight cultures for plasmid purification using the Zyppy Plasmid Miniprep kit (Zymo Research). Sanger sequencing of clones was performed using M13F and M13R primers ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the 96-well Direct-zol MagBead RNA Kit (Zymo Research). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop ...
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bioRxiv - Developmental Biology 2020Quote: Combinations of the AD or BD vectors were co-transformed into yeast using the Frozen-EZ II kit from Zymo Research and using empty vectors as negative controls ...
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bioRxiv - Developmental Biology 2020Quote: ... and stored in −80°C until extraction of total RNA extraction using the Direct-zol™ RNA kit (Zymo Research). The optical density value of total RNA was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... 1ng of each library constructed was used in a polymerase chain reaction with primers flanking the allele and the barcode to generate fragments which were subsequently gel verified and extracted using Zymo gel extraction kit (Zymo). 25ng of the purified product was subjected to self-ligation at 16 °C overnight in a total volume of 50uls and column purified using Qiagen (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was isolated by phenol-chloroform extraction followed by purification using the Genomic DNA Cleanup and Concentration kit from Zymo Research (catalogue number D4065) ...