Labshake search
Citations for Zymo Research :
5201 - 5250 of 6550 citations for Rat LIM Domain Kinase 2 LIMK2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research).
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bioRxiv - Plant Biology 2023Quote: RNA was purified from seedlings Col-0 and cpk32-1 using the Direct-zol RNA MiniPrep Kit (Zymo Research). The reverse-transcriptase cDNA synthesis reaction was carried out by the RevertAid Reverse Transcriptase (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and prepared for total RNA purification using the Quick-DNA/RNA Viral MagBead kit from Zymo Research and an Agilent Bravo NGS A liquid handler(32) ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was harvested from cells 24-hours post-transfection using the Direct-zol RNA Miniprep kits (Zymo Research). Each in vivo splicing assay was performed a minimum of three times.
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bioRxiv - Neuroscience 2023Quote: Hippocampal tissue samples were lysed and bisulfite modified with EZ-96 DNA Methylation-Direct Kit (Zymo Research, CA, USA) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Unmethylated cytosines in the adapter-ligated DNA were chemically converted by EZ DNA Methylation-Gold Kit (Zymo Research Corp.). The CT converted products were purified and subjected to 12 cycles of PCR amplification before single-end sequencing using an Illumina NextSeq500 platform.
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bioRxiv - Biochemistry 2023Quote: ... These cells were cultured in LB media and plasmids were purified using ZymoPURE II Plasmid Midiprep kit (Zymo Research). Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and RNA was extracted using Direct-zol RNA mini kit (Zymo Research, Irvine CA) according to the manufacturer’s instructions and stored in - 70°C till further use ...
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bioRxiv - Biochemistry 2023Quote: ... The methylated and poly-A-tailed RNA samples were purified using an RNA purification kit (Zymo Research, California, USA) with RNase-free water ...
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bioRxiv - Biochemistry 2023Quote: ... Colonies were picked up to amplify before isolating genomic DNA using Quick-DNA miniprep Plus kit (Zymo research, #D4068). Standard PCR with a forward primer (5’-TATCGGTGGTATGCTGTTTCTG-3’ ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were snap-frozen on liquid N2 for RNA extraction performed using Direct-zol RNA MicroPrep kit (Zymo Research).
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bioRxiv - Molecular Biology 2023Quote: ... A portion of the RNA was purified a second time with the RNA Clean & Concentrator-5 kit (Zymo Research), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted using the Direct-zol RNA MiniPrep Kit according to the manufacturer’s instructions (Zymo Research, R2052). The RNA was then reverse transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... A genomic DNA extraction was performed on this clone using the Quick DNA MicroPrep kit (Zymo Research Ref D3020) using manufacturer’s instructions and a subsequent PCR analysis was done using primers amplifying both the LC3B gene and the GFP-edited copy of this gene using Taq Platinium Polymerase Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and the precipitates were subjected to total RNA extraction using the Quick-RNA Fungal/Bacterial Microprep Kit (Zymo Research). DNA removal ...
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bioRxiv - Plant Biology 2023Quote: ... and 400 ng of DNA was treated with bisulfite using the EZ DNA Methylation-Gold kit (Zymo Research, D5006). The DNA regions of interest were PCR-amplified (10 min 95°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted following the total RNA extraction protocol for the Quick-RNA Miniprep kit (Zymo Research, Irvine, CA) and RNA samples were treated by DNase I ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA from filters from the two North Sea datasets were extracted using the Quick-DNA miniprep kit (Zymo, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and WC (anaerobic: n = 131) isolates using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions with the modifications outlined in our previous publication (Webb et al. ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA in cell lysates was extracted using a Quick-RNA extraction miniprep kit (# R1055 Zymo Research, CA, USA) following the manufacturer’s instructions and RNA concentration determined by the nanodrop machine (Thermo Scientific) ...
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bioRxiv - Genomics 2023Quote: ... RNA was extract using either acid phenol extraction (Ares, 2012) or Direct-zol RNA miniprep kit (Zymo Research #R2053). Samples extracted with Direct-zol RNA miniprep kit were disrupted by vortexing with glass beads (Sigma-Aldrich #Z250465 ...
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bioRxiv - Microbiology 2023Quote: ... and 50 µl of the pooled PCR product was purified with the DNA Clean and Concentrator kit (Zymo Research). The final BarSeq library was eluted in 40 µl water ...
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bioRxiv - Genomics 2023Quote: ... depletion by Illumina TruSeg Stranded Total RNA with Ribo-Zero Plus and NEBNext rRNA Depletion Kit (Zymo Genetics, Inc., Seattle, WA, USA). The libraries were sequenced to approximately 9 billion base pairs (bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Products were combined at equimolar concentrations and purified using a Zymo DNA Clean and Concentrator kit (Zymo Research D4014) to create a single multiplexed library for sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The amplicon DNA size was confirmed by gel electrophoresis and purified using the Zymoclean Gel DNA Recovery Kit (Zymo). DNA sequencing library of the purified CASP4 PCR amplicons was prepared using the Native Barcoding Kit (cat no ...
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bioRxiv - Neuroscience 2023Quote: ... Free RNAs were extracted using phenol/chloroform and purified with the RNA Clean & Concentrator-5 kit (Zymo Research, R1015).
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bioRxiv - Cell Biology 2022Quote: ... Triplicate samples were harvested and RNA was extracted using Direct-zol™ RNA MiniPrep Plus kit (Zymo Research, #R2072) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from the frozen sections using the Quick-DNA/RNA Miniprep Plus Kit (Zymo, D7003) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from the PBMC samples using the Direct-Zol RNA MiniPrep kit (Zymo Research, Irvine, CA).
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Bisulfite conversion of genomic DNA (500 ng) was performed using the Zymo Research EZ DNA Methylation kit (Zymo Research) according to the manufacturer’s recommendations for the Illumina Infinium assay ...
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bioRxiv - Genetics 2023Quote: ... 500 ng of genomic DNA was bisulfite converted by the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA), and 4 μl of bisulfite-converted DNA was measured on the Illumina HumanMethylation450 array using the manufacturer’s protocol (Illumina ...
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bioRxiv - Microbiology 2023Quote: RNA from snap-frozen ileum and liver tissues were extracted using the Quick-RNA Miniprep Plus kit (Zymo Research). cDNA was synthesized from 1.5 μg ileum or 2 μg liver RNA using High-Capacity cDNA Reverse Transcriptase kit (Applied Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified for transfection into mammalian cell lines using a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from the shoots and roots using the Direct-zol RNA Mini Prep Kit (Zymo Research). RNA quality and quantity were measured using a NanoDrop ND-1000 spectrophotometer ...
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bioRxiv - Bioengineering 2023Quote: Plasmids of enriched nanobodies were isolated from the terminal round of sorting using a yeast miniprep kit (Zymo, D2004). For nanobody Fc fusions ...
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bioRxiv - Microbiology 2023Quote: Bacterial RNA was extracted from ∼1 × 108 CFU after AMP exposure (above) using Direct-zol RNA Miniprep kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was size-selected to ≥200 nts using the Zymo RNA Clean and Concentrator-5 kit (Zymo #R1013) according to manufacturer’s protocol and eluted in 6 µl of nuclease-free water.
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bioRxiv - Cancer Biology 2023Quote: ... The samples were incubated (37°C, 30 min) and purified with the Zymo RNA Clean & Concentrator Kit (Zymo Research). The concentration of purified RNA was determined (Qubit™ RNA HS Assay Kit) ...
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bioRxiv - Genetics 2023Quote: ... The un-crosslinked sample was ethanol precipitated and purified using the large fragment DNA recovery kit (Zymo Research, D4046). Religated-biotinylated restriction fragments were pulled down using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA (gDNA) was purified from ES cells and tail clips using the Quick-DNA Miniprep Kit (Zymo Research). Initial validation of target insertion was performed with primers designed to amplify across the 5’ and 3’ integration sites (Supplementary data 1-4) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and 150 µL of 100% ethanol and then purified through a column from the purification kit (Zymo Research, R1015) followed by quality check with a Bioanalyzer small RNA kit (Agilent).
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bioRxiv - Synthetic Biology 2023Quote: ... and sequence confirmed (Sequetech) and then maxi-prepped per the manufacturer’s protocols (ZymoPure II Plasmid Maxiprep Kit, Zymo Research). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4 °C.
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bioRxiv - Systems Biology 2023Quote: ... total RNA was extracted from cryosections lysed in Trizol using the Direct-zol RNA Miniprep kit (Zymo Research, R2050) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Colonies were scraped with 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203); the extracted plasmid DNA was then precipitated with isopropanol ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA samples were purified and concentrated using an RNA Clean & Concentrator-5 Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... DNA was purified from the frozen liver using the Quick-DNA Miniprep Plus kit (D4068, Zymo Research, Tustin, CA). After quality and quantity checks ...
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bioRxiv - Synthetic Biology 2024Quote: ... Bulk RNA was isolated from sorted cells and cells with the original library (Direct-zol RNA Microprep Kit, Zymo) and used as a template for cDNA synthesis (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA and RNA were extracted from specimens in parallel using the Quick-DNA/RNATM FFPE miniprep kit (Zymo Research). Nucleic acid concentration was determined with a Qubit™ 1xDNA or RNA high sensitivity kit (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... The total RNA and RF samples were purified using a modified RNA Clean & Concentrator-5 kit method (Zymo Research). The volume of each sample was increased to 100 µl ...