Labshake search
Citations for 3M :
101 - 150 of 189 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... Post-hybridization washes were undertaken with 0.005x (ZmSCR1) and 0.01x (ZmSCR1h) SSC buffer made from a 20x SSC stock (3M NaCl, 0.3M Na3citrate), calculated to ensure stringency.
-
bioRxiv - Cancer Biology 2019Quote: ... by overnight capillary transfer at RT using 20X SSC transfer buffer (3M NaCl, 0.3M Sodium citrate, pH 7). DNA was fixed on the membrane by UV-crosslinking at 1.2J/cm2 for 30s in HL-2000 Hybrilinker (UVP ...
-
bioRxiv - Cancer Biology 2019Quote: ... by overnight capillary transfer at RT using 20X SSC transfer buffer (3M NaCl, 0.3M Sodium citrate, pH 7). DNA was fixed on the membrane by UV-crosslinking at 1.2J/cm2 for 30s in HL-2000 Hybrilinker (UVP ...
-
bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
-
bioRxiv - Molecular Biology 2019Quote: ... Insoluble precipitates were removed by centrifugation and soluble crosslinked chromatin extracts were diluted 10x in IP buffer (0.3M Sucrose ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were washed rapidly with PBS before incubation (at least 30 min) with pre-hybridization buffer : 15% formamide in 2X SSPE pH8.0 (0.3M NaCl ...
-
bioRxiv - Bioengineering 2020Quote: ... samples were incubated in penetration buffer (0.2% Triton X-100, 20% DMSO, and 0.3M glycine in 1X PBS), then blocking buffer (0.2% Triton X-100 ...
-
bioRxiv - Genomics 2019Quote: ... 6uL of 2x hybridization buffer (40% dextran sulfate in 8x SSC (20x SSC: 3M NaCl, 0.3M Sodium citrate)) was then added to each probe ...
-
bioRxiv - Genomics 2019Quote: ... 6uL of 2x hybridization buffer (40% dextran sulfate in 8x SSC (20x SSC: 3M NaCl, 0.3M Sodium citrate)) was then added to each probe ...
-
bioRxiv - Developmental Biology 2022Quote: ... Insoluble precipitates were removed by centrifugation and soluble crosslinked chromatin extracts were diluted 10x in IP buffer (0.3M Sucrose ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell pellet was washed once with Disruption Buffer (20mM Tris-HCl pH7.9, 10mM MgCl2, 1mM EDTA, 10% glycerol, 0.3M ammonium sulfate and protease inhibitor) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the column is washed three times total: once using 400μL RNA Prep Buffer (3M GuHCl in 80% EtOH), twice with 400μL 80% ethanol ...
-
bioRxiv - Developmental Biology 2022Quote: ... about 50 μl of pelleted cross-linked cells were resuspended in 350 μl sarkosyl lysis buffer (0.25% sarkosyl, 1 mM DTT and Protease Inhibitor Cocktails in 0.3M RIPA buffer and sonicated at 60% amplification by a tip sonicator (125W ...
-
bioRxiv - Neuroscience 2023Quote: ... chromatin was eluted by resuspending beads in 100 ul of Elution buffer (10 mM Tris pH 8.0, 5mM EDTA, 0.1% SDS, 0.3M NaCl) supplemented with 50ugr of proteinase K and incubating at 65C overnight in an agitating thermoblock with heated lid ...
-
bioRxiv - Biochemistry 2023Quote: The embryo derivative samples (Embryos, in situ NuMat, and in situ NuMat extracted 1M, 2M, 3M GdnHCl buffers) were blocked by rotating in 3% BSA in PBT for 2 hours at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells were permeabilized with 0.1% Triton X-100 for 15 minutes at RT and blocked with IF buffer (1.3M NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed twice with PBS before being exposed to pre-extraction buffer (25 mM HEPES pH 7.4, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton-X-100, 0.3M Sucrose) for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were washed rapidly with PBS before incubation for at least 30min with pre-hybridization buffer (15% formamide in 2X SSPE pH8.0 [0.3M NaCl ...
-
bioRxiv - Genomics 2023Quote: ... They were then allowed to air-dry before addition of digestion buffer (3M Urea, 100 mM HEPES, pH8). Samples were resuspended with sonication (as above) ...
-
bioRxiv - Genetics 2024Quote: ... Homogenate was then transferred to a pre-chilled 15mL conical tube and mixed with 6mL Buffer B (2.3M sucrose ...
-
Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... resuspended in 50 μl of sample buffer with urea (50 mM Tris-HCl, pH 6.8, 100 mM DTT, 3% SDS, 3M urea ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
-
bioRxiv - Neuroscience 2020Quote: ... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
-
bioRxiv - Plant Biology 2021Quote: Mitochondrial respiration rate measurements were performed using the Clark-type oxygen electrode (Hansatech) with 120 - 240 μg of enriched mitochondria in 1 mL of mitochondrial respiration buffer (0.3M Sucrose ...
-
bioRxiv - Cell Biology 2019Quote: ... the cultures were fixed using cold paraformaldehyde (4%) for 1 hr and then permeabilized using a buffer containing digitonin (50 µg/ml)/sucrose (0.3M) (22 ...
-
bioRxiv - Biochemistry 2021Quote: ... The crude F1 preparation was loaded onto a 5mL HisTrap HP 5 mL column at 4 °C and washed with 300 mL of 97 % wash buffer (10% MeOH, 10% glycerol, 0.3M NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (27) ...
-
bioRxiv - Biochemistry 2022Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (44) ...
-
bioRxiv - Plant Biology 2022Quote: ... Post-hybridization washes were undertaken with 0.005 x SSC buffer made from a 20x SSC stock (3M NaCl, 0.3M Na3citrate), calculated to ensure stringency.
-
Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Antibodies were then eluted by incubating the samples in an elution buffer (0.5 M L-glycine, 3 M Urea, 3M Guanidium Chloride ...
-
bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate ...
-
bioRxiv - Developmental Biology 2023Quote: ... in each round the sample was incubated for ten minutes at room temperature in an elution buffer (0.5M L-Glycine, 3M urea, 3M guanidine hydrochloride ...
-
bioRxiv - Developmental Biology 2023Quote: ... in each round the sample was incubated for ten minutes at room temperature in an elution buffer (0.5M L-Glycine, 3M urea ...
-
bioRxiv - Cell Biology 2023Quote: ... the column is washed three times total: once using 400 μL RNA Prep Buffer (3M GuHCl in 80% EtOH), twice with 400 μL 80% ethanol ...
-
bioRxiv - Biophysics 2023Quote: ... Both reservoirs were filled with 400μl of measurement buffer each (3M KCl, 100mM Tris, 0.1 mM EDTA, pH 8), connected to Ag/AgCl electrodes (silver wire with 0.5mm diameter ...
-
bioRxiv - Plant Biology 2023Quote: ... Post-hybridization washes were undertaken with 0.005 x SSC buffer made from a 20x SSC stock (3M NaCl, 0.3M Na3citrate), calculated to ensure stringency.
-
bioRxiv - Biochemistry 2023Quote: ... and subsequently eluted with 5–10 bead volumes of elution buffer (0.1M Tris [pH 8.0], 0.3M NaCl, 250mM Imidazole). Ni-NTA eluates was concentrated using amicon ultra concentrators ...
-
bioRxiv - Genomics 2023Quote: ... FAC-sorted cells were pelleted at 600 rcf for 10 minutes in a swinging bucket centrifuge at 4°C and resuspended in cold Buffer I (0.3M Sucrose ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with ten column volumes of elution buffer (0.1M Tris HCl, pH 7.5, 0.3M Nacl, 250mM imidazole) in fractions of 1.0 ml in DNA/protease-free Eppendorf tubes ...
-
bioRxiv - Genetics 2023Quote: ... Fixed cells were incubated in blocking buffer (1% BSA, 2% FCS, 0.3M glycine and 0.1% Tween-20 in PBS) at room temperature for 1 hour ...
-
bioRxiv - Physiology 2023Quote: ... Samples were dehydrated in an increasing methanol (MeOH) gradient (20%→40%→60%→80%→(2x)100%) v/v in B1n buffer (0.3M glycine ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were rinsed three times with dH20 and incubated in equal amounts of dH20 and elution buffer (3×5min; 0.5M L-glycine, 3M urea, 3M guanidine hydrochloride ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were rinsed three times with dH20 and incubated in equal amounts of dH20 and elution buffer (3×5min; 0.5M L-glycine, 3M urea ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The solution is placed back on the magnet for 5 minutes and the supernatant containing the non-biotinylated strand is removed gently with non-stick pipettes and mixed immediately with Neutralization Buffer (100 uL 3M Sodium Acetate pH 5.2 and 4.9 mL 1X TE Buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then washed 3 times 3 min in Tris buffer saline (TBS; Tris 1M, NaCl 3M and pH 7.5) and incubated 20 min in 0.3% hydrogen peroxide to block endogenous peroxidases ...
-
bioRxiv - Cell Biology 2021Quote: ... washed with water and resuspended in glass bead disruption buffer (20mM Tris-HCl pH 8.0, 10mM MgCl2, 1mM EDTA, 5% Glycerol, 0,3M ammonium sulfate) supplemented with 1mM DTT ...
-
bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
-
bioRxiv - Biophysics 2022Quote: ... an aliquot from the exchanging experiment was sampled and diluted 1:13 into D2O quenching buffer containing (100 mM phosphate, pH 2.1, 50 mM NaCl, 3M GuHCl) at 1 °C ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The clarified peptide flow-throughs were separated from the beads and the beads were reconstituted in 200 mL IMAC binding/wash buffer (80 MeCN/0.1% TFA) and loaded onto equilibrated Empore C18 silica-packed stage tips (3M, 2315). The samples were then washed twice with 50 μL of IMAC binding/wash buffer and once with 50 μL of 1% FA and eluted thrice from the IMAC beads to the stage tips with 70 μL of 500 mM dibasic sodium phosphate (pH 7.0 ...
-
bioRxiv - Immunology 2020Quote: ... Beads were resuspended in 150 μl Direct elution buffer (10mM Tris-HCl pH8, 0.3M NaCl, 5mM EDTA pH8, 0.5%SDS) and incubated overnight at 65°C and input samples were included ...