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Citations for 3M :
51 - 100 of 189 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Beads were resuspended by vortexing in 5 µL of digestion buffer (3M GuaHCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel slices were rotated at 4°C overnight in RNA Elution Buffer (0.3M NaOAc pH 5.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... isolated and crosslinked nuclei were MNase digested in 400µl MNase digestion buffer (0.3M Sucrose ...
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bioRxiv - Genetics 2023Quote: ... 200 µl of MURBs buffer (50 mM sodium phosphate, 25 mM MES, 3M urea ...
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Novel model of distal myopathy caused by the myosin rod mutation R1500P disrupts acto-myosin bindingbioRxiv - Molecular Biology 2019Quote: Protein lysates were prepared by homogenizing hindlimb muscle tissue in myosin extraction buffer (0.3M NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... and cytoplasmic fraction was released by incubation with 1.5 ml of lysis-buffer (0.3M sucrose ...
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bioRxiv - Developmental Biology 2022Quote: ... were lysed in hypotonic buffer (10mM Tris pH 8, 2mM MgCl2, 24mM CaCl2, 0.3M sucrose ...
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bioRxiv - Immunology 2022Quote: ... Samples were resuspended in 200 µL elution buffer 1 (EB1, 50% ACN, 0.3M NH4OH) and incubated for 1 hour on a ThermoMixer at 45°C ...
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bioRxiv - Immunology 2020Quote: ... having been washed with low cold salt extraction buffer (0.3M sodium phosphate, pH 7.6), was treated with 1:5 trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... Roughly 40-50g of tissue was homogenized in pre-chilled grinding buffer B (0.3M sucrose ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized by incubation in blocking buffer (0.05M PBS, 0.3M glycine, 5% donkey serum) containing one of two detergents ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked in 10% FBS in immunofluorescence (IF) buffer (0.3M glycine in PBS) for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the degelation step is immediately initiated by adding 3.75 mL of degelation buffer (3M guanidine thiocyanate and anti-foam reagent supplied to the reverse transcription reaction buffer (RT buffer) ...
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bioRxiv - Genetics 2024Quote: Protein lysates or histone samples were mixed with 5X Loading Buffer (5% SDS, 0.3M Tris pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... followed by another wash in 20X SSC buffer (3M NaCl, 0.3M sodium citrate, pH 7.0) for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... followed by another wash in 20X SSC buffer (3M NaCl, 0.3M sodium citrate, pH 7.0) for 15 min ...
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bioRxiv - Immunology 2021Quote: The slides were equilibrated with 300μl buffer 2xSSC (0.3M NaCl, 0.03M sodium citrate, pH=7.0) 2x 3mins ...
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bioRxiv - Microbiology 2019Quote: ... re-suspended in 2 ml of NTA buffer (50 mM NaH2PO4, pH 8.0, 0.3M NaCl) containing 2 mg lysozyme ...
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bioRxiv - Molecular Biology 2020Quote: ... equilibrated and eluted with SEC buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP). The BTB domain of PLZF (Ahman ...
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bioRxiv - Cancer Biology 2020Quote: Harvested cell culture pellets were resuspended in protein lysis buffer (25mM HEPES pH 7,5; 0,3M NaCl ...
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bioRxiv - Immunology 2022Quote: ... Beads were then reconstituted in 200 µL elution buffer 2 (EB2, 5% ACN, 0.3M NH4OH) and incubated for 1 hour on a ThermoMixer at 45°C ...
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bioRxiv - Cell Biology 2019Quote: ... then cells were treated with RNase in 2XSSC buffer (0.3M NaCl, 30mM Sodium Citrate, pH7.0), dehydrated and denatured under 72°C following cold ethanol wash ...
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bioRxiv - Molecular Biology 2019Quote: ... The chromatin was then isolated by centrifugation and washed twice in MNase digestion buffer (0.3M Sucrose ...
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bioRxiv - Biochemistry 2023Quote: NuMat was subfractionated by sequential extraction with GdnHCl extraction buffers (1M, 2M, 3M, 4M, 8M) as per the experimental schematic shown in Fig 1 a ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclei were incubated with blocking buffer (1% Normal Donkey Serum in PBS + 0.3M glycine) for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... and 200 U/L of ACE in 0.1M sodium borate buffer (pH 8.3, 0.3M NaCl). Six inhibitor concentrations were tested (5 ...
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bioRxiv - Molecular Biology 2020Quote: ... Reaction was stopped with 100 µL of Buffer A (0.3M NaOAc and 0.1mM EDTA, pH 5.6), 700 µL absolute ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Then the cells were incubated with blocking buffer (1XPBS, 1% BSA, 0.3M glycine, 0.1% Tween 20) supplemented with 5% goat serum for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Hybridized membranes were washed 2x with 20x SSC buffer (3M NaCl, 300 mM NaCit, pH 7.0), washed 3x with 2x SSC ...
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bioRxiv - Biochemistry 2022Quote: ... gel fractions corresponding to 20–30 nt were harvested and crushed in extraction buffer (0.3M NaCl). After overnight rotation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining pellet was washed with the washing buffer (10mM HEPES pH7, 50mM NaCl, 0,3M sucrose) supplemented with protease inhibitors cocktail (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 5 CV of high salt buffer (25 mM Tris-HCl, pH 7.5, 3M NaCl). After re-equilibration of the column with buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear extracts were extracted at 4 C in high salt buffer (20mM Tris pH7.9, 1.5mM MgCl2, 25% glycerol, 0.2mM EDTA, 0.5mM DTT and 0.3M NaCl) followed by centrifugation at 6000g ...
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bioRxiv - Developmental Biology 2024Quote: ... 400 µL MNase complete buffer (85 mM Tris-HCl pH 7.5, 3mM MgCl2, 2mM CaCl2, 0.3M Sucrose ...
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bioRxiv - Molecular Biology 2024Quote: ... 2X PK buffer (0.2M Tris-HCl [pH 7.5], 25mM EDTA, 0.3M NaCl, 2% [w/v] SDS) was added to stop the reaction ...
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bioRxiv - Immunology 2021Quote: ... The membrane was then incubated in AC buffers with decreasing concentrations of Guanidine HCl (3M, 1M, 0.1M) for 30min each ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclei pellet were resuspended in an equal volume of Nuclear Lysis Buffer (Hepes 1M, pH7.6, KCl 3M, EDTA 0.5M ...
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bioRxiv - Neuroscience 2019Quote: ... PC12 cells were lysed in ice-cold gradient buffer (0.3M NaCl, 1mM MgCl2, 15mM Tris-HCl (PH7.4), 0.1mg/mL cyclohexamide and 1mg/mL heparin ...
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bioRxiv - Biophysics 2023Quote: ... Different concentrations of protein samples were mixed with 4X Loading buffer (0.25 M Tris-HCl pH 6.8, 8% SDS, 0.3M DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Briefly: The cell pellet containing expressed untagged RAD15 was thawed in two volumes of lysis buffer (3M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and five column volumes of Buffer B (A (0.1M Tris HCl, pH 7.5, 0.3M Nacl, 50mM imidazole) respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Washes were then performed with Wash Buffer (Molecular Instruments) at 37°C followed by 5x SSCT (3M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... and eluted with Direct Elution Buffer (10mM Tris-HCl pH8, 0.3M NaCl, 5mM EDTA pH8, 0.5%SDS). The chromatin was crosslink reversed by heating at 65 °C for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel containing RNA was incubated overnight at 42°C in elution buffer (0.3M NaOAc pH 5.5, 2% SDS). RNA was ethanol precipitated and stored at -20°C until use.
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed with 1 x SDS sample buffer (0.32 M Tris pH 6.8, 10% SDS, 50% glycerol, 3M β-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... Ni-NTA resin was resuspended in 1mL wash buffer (20mM imidazole, 5mM NaH2PO4.H2O, 0.3M NaCl in H20), transferred to a 2mL microcentrifuge tube ...
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bioRxiv - Cancer Biology 2021Quote: ... Between 10 to 20 × 108 freshly harvested RMC2C cells bearing either SMARCB1 or mCHERRY and treated 2uM doxycycline for 48hrs were resuspended in 1.5 ml ice-cold hypotonic buffer (0.3M Sucrose ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 50mL MURB protein sample buffer (100mM MES pH7.0, 1% SDS, 3M urea, 10% 2-mercaptoethanol) prior to Western analysis.
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bioRxiv - Molecular Biology 2022Quote: 1 ml of frozen blood sample is thawed and lysed in 10 ml of the NUN buffer (0.3M NaCl ...