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Citations for Expression Systems :
201 - 221 of 221 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Baculoviruses encoding CSB variants, CSA/DDB1, or CUL4A/RBX1 were amplified in three stages (P1, P2, and P3) in Sf9 cells (Expression Systems). Protein expression was performed for 72 h in 500 ml Sf9 cells per construct infected with 10 ml P2 or P3 baculovirus ...
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bioRxiv - Immunology 2019Quote: All TEP1 and LRIM1/APL1C constructs were expressed using the baculovirus expression system using T.ni cells in ESF-921 media (Expression Systems LLC). TEP1 was expressed for ~60 h at 27°C ...
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bioRxiv - Molecular Biology 2021Quote: ... BriSΔ was produced with the MultiBac baculovirus expression system (Geneva Biotech)43 in Hi5 cells using ESF921 media (Expression Systems Inc.). Supernatants from transfected cells were harvested 3 days post-transfection by centrifugation of the culture at 1000g for 10 min followed by another centrifugation of supernatant at 5000g for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... were transiently transfected with ExpiFectamine into 2.5×107 Expi293F cells in 10ml medium according to manufacturer’s protocol (Gibco Expi293 Expression System, Thermo Fisher). One day after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... human full-length CB1 containing a N-terminal FLAG tag and C-terminal histidine tag was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Receptor was extracted using 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PfMyoA was expressed as described previously [17] in Sf9 cells adapted to serum-free ESF 921 media purchased from Expression Systems. Sf9 cells were co-infected with PfMyoA ...
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bioRxiv - Biochemistry 2023Quote: ... The human heterodimeric Gβ1γ2 was expressed in Spodoptera frugiperda Sf9 insect cells and the anti-Bril Fab was expressed in secreted form from Trichuplusia ni Hi5 insect cells using the baculovirus method (Expression Systems), respectively ...
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bioRxiv - Biophysics 2022Quote: ... Viral titers were performed by the flow cytometric method23 on a Guava easycyte 8HT in which cells were stained with Anti-gp64-PE antibody (Expression Systems).
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bioRxiv - Biochemistry 2023Quote: ... individual Orc1 constructs were co-transfected with vectors p8HCO (providing a methotrexate resistant gene) and pCopiaFP(mTurquoise2)-DmHis2A into Drosophila S2 cells (Expression Systems) maintained at 27°C in ESF 921 medium (Expression Systems) ...
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bioRxiv - Biochemistry 2024Quote: The HormR+GAIN/7TM construct of ADGRL3 was expressed in Sf9 insect cells grown in ESF 921 serum-free media (Expression System). Cells grown to a density of 4.0 × 106 cells ml−1 were infected with high-titer baculovirus ...
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bioRxiv - Biophysics 2024Quote: ... Bacmid was purified using the manufacturer’s instructions followed by transfection in Sf9 cells grown in suspension using ESF921 media (Expression Systems, CA) with X-tremeGENE HP transfection reagent (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... either control or Per2 shRNA as well as viral packaging vectors were transfected into HEK293/T17 cells according to the manufacturer’s instruction (ViraPower Lentiviral Expression System, Life Tech). Cell culture media was collected after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Omicron-SARS-CoV-2 spike RBD and ACE2 variants (CVD293 and CVD432) were expressed in high density Expi293FTM cells in Expi293TM expression media following manufacturer’s protocol (Expi293 Expression System, ThermoFisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... β1 and unlipidated (C68S mutation) γ2 subunits were co-expressed in HighFive (Trichoplusia ni) cells (Expression Systems; we did not test for mycoplasma). The protein was purified via Ni2+-affinity chromatography followed by anion exchange chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... or 2% vol/vol PI3Kγ (PIK3CG/PIK3R5) baculovirus and grown at 27ºC in ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001) for 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... or APP624 was optimized using the TEQC method [37] in which a 200 ml culture of Hi5 cells (Expression Systems, Inc, 94-002S) was infected at an estimated multiplicity of infection (eMOI ...
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bioRxiv - Cell Biology 2019Quote: ... elegans embryos were permeabilized using perm-1 RNAi (Carvalho et al., 2011) and dissected into a 62% solution of ESF-921 Media (Expression Systems). To arrest the embryos at metaphase ...
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bioRxiv - Biochemistry 2020Quote: ... The percentage of cells infected by virus was quantified by flow cytometry following staining of 10 µL of cells with 10 µL of PE-conjugated anti-gp64 antibody (Expression Systems, catalog #97-201) for 20 min in the dark at 4°C ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Biochemistry 2021Quote: ... insect cells plated for 24 hours in a Corning 6-well plastic dish (Cat# 07-200-80) containing 2 mL of ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001, Davis, CA.). All media contains 1x concentration of Antibiotic-Antimycotic (Gibco/Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... insect cells plated for 24 hours in a Corning 6-well plastic dish (Cat# 07-200-80) containing 2 mL of ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001, Davis, CA.). All media contains 1x concentration of Antibiotic-Antimycotic (Gibco/Invitrogen ...