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Citations for Expression Systems :
1 - 50 of 221 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Biochemistry 2019Quote: ... or GST-tagged BMAL1 PAS-AB were expressed in Sf9 suspension insect cells (Expression Systems). Sf9 suspension cells were infected with a P3 virus at 1.2 × 106 cells per milliliter and grown for 72 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was performed by transducing recombinant baculoviruses into Sf21 insect cells (Expression Systems). Cells were harvested by centrifugation and resuspended in 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... A recombinant baculovirus (P0) was produced in SF9 cells (Expression Systems, CA, USA) as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems ...
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bioRxiv - Cell Biology 2021Quote: ... First passage (P1) recombinant baculovirus was generated by transfecting Sf9 cells (Expression Systems) with bacmid DNA complexed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Biophysics 2023Quote: ... recombinant FUS was expressed in Sf9 insect cells (Expression Systems, Cat#94-001F) for 72 h using the baculovirus expression system87 and produced as a C-terminal SNAP fusion with an N-terminal maltose-binding protein (MBP ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant P1 baculovirus DNA was transfected into and cultured in Sf9 cells (Expression System) in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems ...
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bioRxiv - Microbiology 2023Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in the protein free ESF 921 Insect Cell Culture Medium (Expression System) and infected with 0.8-1.0% (v/v ...
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bioRxiv - Biochemistry 2022Quote: DHX9-His was expressed by transducing 800 ml Tni cell culture in ESF921 serum-free media (Expression Systems) at a density of 1×106 cells/ml with 16 ml baculoviral suspension (generated in Sf9 cells ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were infected with recombinant baculovirus (BestBac Expression Systems) at a density of ∼4.0 × 106 cells per ml ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: Expression of recombinant RDR2 was optimized using the TEQC method (62) in which a 200 ml culture of Hi5 cells (Expression Systems, Inc) was infected at an estimated multiplicity of infection (eMOI ...
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bioRxiv - Biochemistry 2020Quote: ... A recombinant baculovirus for Gαs subunit was prepared using the BestBac™ (Expression Systems) strategy ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... one liter of cells (2.5×106 cells ml−1, medium from Expression Systems) was infected with 20 ml baculovirus at 28 ℃ ...
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bioRxiv - Immunology 2020Quote: 50 ml of sf21 cells (2.5×106 cells ml−1, medium from Expression Systems) were infected with 1 ml baculovirus of GST-rNLRP1FIIND wild-type or mutants ...
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then used to infect 1 L of Sf21 cells (Expression Systems) and incubated for 48 hrs at 27°C ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems). Next ...
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bioRxiv - Biochemistry 2023Quote: Mouse CRY1 PHR domain (residues 1-491) was expressed in Sf9 suspension insect cells (Expression Systems) as previously described (Parico et al. ...
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bioRxiv - Biophysics 2019Quote: ... 13 mL of the second amplification was used to infect 1 L of Sf21 cells (Expression Systems) at 2.8 × 106 cells/mL and incubated at 27°C for 48 hours ...
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bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...
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bioRxiv - Immunology 2022Quote: ... 500 ml SF9 cells (∼106 cells/ml; Expression Systems, 94-001F) were infected with high-titer baculoviral supernatant ...
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bioRxiv - Biochemistry 2022Quote: ... When the cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems), we infected the cells with five separate virus preparations at a ratio of 1:2:2:2:2 for PTH1R(27-502)-LgBiT-2MBP ...
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bioRxiv - Biochemistry 2023Quote: ... The Sf9 cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems) for expression ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were kept in ESF 921 Insect Cell Culture Medium (Expression systems) that was supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... Sf9 cells (Expression System) were grown in ESF921 medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... sf9 cells (Expression System) was infected with baculovirus at a density of 4×106 cells/mL and incubated for 60 hours at 27 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Sf9 cells (Expression Systems) were infected at a density of 3 × 106 cells/mL with 1:300 v/v virus and harvested after 48 hours ...
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bioRxiv - Biochemistry 2024Quote: Sf9 cells (Expression System) were maintained in Sf-900™ II serum-free medium (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... Sf9 cell cultures were grown to cell density at about 4×106 cells/ mL in ESF 921 serum-free medium (Expression Systems). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: Sf9 insect cells (Expression Systems) were grown in suspension in ESF 921 media (Expression Systems ...
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bioRxiv - Biochemistry 2022Quote: ... Sf9 insect cells (Expression Systems) were infected with a BestBac-derived baculovirus at a density of 3.5 x 106 cells/mL and harvested 48 hours post infection ...
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bioRxiv - Biochemistry 2022Quote: ... High Five cells (Expression Systems) in suspension culture at a density of 1×106 cells/ml were infected with baculovirus (V1 ...
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bioRxiv - Biochemistry 2023Quote: ... ni insect cells (Expression Systems) and purified as described above.
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bioRxiv - Biophysics 2021Quote: ... Sf9 cells were seeded at 2 x 106 cells / mL in ESF-921 (Expression Systems), and infected simultaneously with myosin-15 baculovirus at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2019Quote: ... Cells were cultured in ESF 921 Insect Cell Culture Medium (Expression systems #96-001-01). Frozen cell line stocks were taken from −80°C freezer and thawed quickly at 27°C in a water bath ...
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bioRxiv - Biochemistry 2021Quote: ... and High Five cells were maintained in ESF 921 Insect Cell Culture Medium (Expression System). Human embryonic kidney 293T cells (HEK-293T ...
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bioRxiv - Cell Biology 2023Quote: Retriever was expressed by co-infecting Sf9 cells at 2 M/mL cell density (Expression Systems) with individual baculoviruses prepared by the Bac-to-Bac system used in other studies58 ...
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bioRxiv - Cell Biology 2023Quote: ... frugiperda insect cells grown at 27°C in ESF 921 Insect Cell Culture Medium (Expression Systems) supplemented with Fetal Bovine Serum (2% final concentration) ...
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bioRxiv - Cell Biology 2023Quote: ... frugiperda insect cells grown at 27°C in ESF 921 Insect Cell Culture Medium (Expression Systems) supplemented with Fetal Bovine Serum (2% final concentration) ...
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bioRxiv - Biophysics 2021Quote: ... in sf9 insect cells (Expression Systems). High Five™ cells (ThermoFisher ...
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells (Expression Systems, 94-005) were maintained at 25°C in Schneider’s Drosophila medium (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... The Sf9 insect cells (Expression Systems) at a density of 2.4 million/ml were infected with both Gαi and Gβγ virus in a 1:1 ratio ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Tni and Sf9 cells (Expression Systems) were maintained in ESF-921 media (Expression Systems ...
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bioRxiv - Biophysics 2023Quote: ... High-five insect cells (Expression Systems) and Expi293F mammalian cells (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: Spodoptera frugiperda (Sf9) insect cells (Expression Systems) were grown in ESF 921 serum-free medium (Expression Systems ...