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Citations for Expression Systems :
151 - 200 of 221 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... were cultured in ESF media (ESF 921 Insect Cell Culture Medium, Expression Systems) with 5% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Trichoplusia ni Hi5 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems) were infected at a density of 3.0 x 106 cells/mL with high titer Gβ1γ2-baculovirus ...
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bioRxiv - Biochemistry 2020Quote: ... cell suspension cultures adapted to grow in a serum-free medium (ESF, Expression Systems). Transfections were performed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Biophysics 2022Quote: ... for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems) at 27°C with ambient CO2 and gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Trichoplusia ni (T. ni) insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems) were co-infected with two baculoviruses at a density of ∼3.0 × 106 cells per ml ...
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bioRxiv - Biochemistry 2022Quote: ... in Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems). For expression ...
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bioRxiv - Biochemistry 2023Quote: ... in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems) in the presence of PEI ...
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bioRxiv - Biochemistry 2024Quote: ... were cultured in protein free ESF 921 insect cell culture media (Expression Systems LLC). Bacmid preparations ...
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bioRxiv - Biochemistry 2023Quote: Sf9 cells were grown at 27 °C in suspension within ESF293 medium (Expression Systems), supplemented with 0.06 mg/ml penicillin G and 0.1 mg/ml streptomycin (sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 and insect cell culture media were purchased from Expression Systems (Davis, CA, USA). Fetal bovine serum was purchased from PEAK (Wellington ...
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bioRxiv - Biophysics 2021Quote: Baculovirus expressing SSTR2KappaICL3-GFP at P2 was used to infect Sf9 insect cells (Expression Systems) at a density of 3–4 million cells/ml and incubated at 28C with shaking ...
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bioRxiv - Molecular Biology 2021Quote: HMCES-FLAG proteins were expressed in 250 mL suspension cultures of Sf9 cells (Expression Systems) by infection with baculovirus expressing rHMCES-FLAG for 72 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... Trichoplusia ni (T. ni) insect cells using baculoviruses generated by the BestBac method (Expression Systems) were used for expression of Gs heterotrimer ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were infected with baculovirus (prepared using Bac-to-Bac Baculovirus Expression System, Invitrogen) containing either the mutant ACKR3 or CXCR4 ...
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bioRxiv - Neuroscience 2021Quote: ... Subsequent steps used Spodopetera frugiperda SF9 cells cultured in ESF 921 medium (Expression Systems, Davis, CA). Bacmid was transfected into adherent SF9 cells using the Escort IV (MillaporeSigma ...
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bioRxiv - Biochemistry 2020Quote: ... Sf9 and High Five cells were cultured in ESF 921 medium (Expression Systems, 96-001-01) in a shaker at 140 rpm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Viral titres were analysed by flow cytometry on cells stained with gp64-PE antibody (Expression Systems). Human GLP1R ...
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bioRxiv - Immunology 2024Quote: ... Stable polyclonal S2 insect cells lines were generated through transient transfection with ExpreS2TR reagent (Expression Systems) mixed with the relevant plasmid and subsequent culturing under selection with G418 (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Titers were determined by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). Expression of AE1 for structural studies was carried out by infection of Sf9 cells at a cell density of 2-3 ×106 cells/ml with P1 virus at MOI (multiplicity of infection ...
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bioRxiv - Biophysics 2021Quote: ... and scFv16 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... and Gγ2 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were obtained from Expression Systems and maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... This CB1R construct was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Insect cell pellets expressing CB1R was solubilized with buffer containing 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2024Quote: ... Spodoptera frugiperda Sf9 cells were transfected with specific plasmid and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi5 cells grown in ESF-921 media (Expression Systems, Davis, CA, United States) at 27 °C ...
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bioRxiv - Cell Biology 2021Quote: ... were grown to a density of 4x106 cells/mL in ESP 921 media (Expression Systems, 96- 001-01) at 27 °C ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C and 100 rpm agitation in ESF921™ insect cell-culture medium (Expression Systems), keeping cell density between 0.4 - 2.5 × 106 cells/ml ...
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bioRxiv - Biochemistry 2023Quote: Co-expression of MeSufDSUC and MeSufDSUBC was performed in Spodoptera frugiperda ExpiSf9 cells (ExpiSf™ Expression System, ThermoFisher) in ExpiSfCD media through infection with baculovirus according to manufacturer’s instructions (Bac-to-Bac™ Baculovirus Expression system ...
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bioRxiv - Biophysics 2023Quote: ... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Approximate titers were estimated by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). All protein for this study ...
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bioRxiv - Biophysics 2019Quote: ... 1 microgram of bacmid DNA was diluted in ESF 921 media (Expression systems, no antibiotic or serum), mixed with 6 μL of Fugene HD transfection reagent (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Baculovirus was generated using Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems 94-001F) and the construct was expressed in Spodoptera frugiperda Sf9 insect cells ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of each plasmid was transfected into SF9 insect cells with BestBac 2.0 linearized baculovirus DNA (Expression Systems) using Cellfectin II reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Spodoptera frugiperda ovarian-derived Sf9 cells were maintained in suspension culture in ESF 921 media (Expression Systems, Davis, CA) and in adherent culture in Grace’s media (Gemini Bio-Products ...
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bioRxiv - Molecular Biology 2024Quote: Baculovirus encoding Halo-Ku80/Ku70 was amplified in three stages (P1, P2, and P3) in Sf9 cells (Expression Systems). To express X ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... GIPR and Gs heterotrimer were co-expressed in High Five™ cells grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... Plasmids for each chain were separately transfected into SF9 insect cells with BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of plasmid DNA was transfected into SF9 insect cells using BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Spodoptera frugiperda Sf9 insect cells were co-transfected with the constructed plasmids and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Tni cells were used for large-scale protein expression and are maintained in ESF 921 serum free medium (Expression Systems).
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bioRxiv - Biochemistry 2023Quote: ... The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems), followed by purification through Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: For expression of Nv-Osk and Nv-Vas proteins in S2 (Drosophila Schneider 2) cells (Expression Systems, cat # 94-005S), corresponding cDNAs were subcloned under control of Actin5C promoter (GenScript) ...
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bioRxiv - Molecular Biology 2020Quote: ... were subcloned into pAC8-derived vectors34 and baculovirus was generated in Spodoptera frugiperda Sf9 cells grown in ESF921 medium (Expression systems) following the manufacturer’s manual ...
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bioRxiv - Biophysics 2019Quote: ... with N-terminal FLAG and C-terminal octahistidine tag was expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus method (Expression Systems) in the presence of the L-168,049 (Tocris Bioscience) ...
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bioRxiv - Biophysics 2019Quote: ... heterotrimeric Gs was expressed in High Five™ (Trichuplusia ni) insect cells using baculoviruses generated by the BestBac method (Expression Systems). Two separate baculoviruses were used ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Both wild-type and kinase-dead (D286A) Sgk3 were expressed in baculvirus-infected Sf9 insect cells grown in ESF 921 medium (Expression Systems) at 27°C ...
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bioRxiv - Bioengineering 2022Quote: ... All baculovirus-based constructs in this work were expressed for 48 hours by infecting HiFive Trichoplusia ni insect cell cultures (Expression Systems) at a density of 2×106 cells/mL with infective baculovirus particles ...
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bioRxiv - Molecular Biology 2019Quote: One liter of Sf9 cells infected with P3 viral particles expressing each construct was grown in ESF 921 medium (Expression Systems) at 28°C ...