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Citations for Electron Microscopy Sciences :
401 - 450 of 529 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and allowed to completely dry before staining with either a Sato’s Lead solution (1% lead acetate (EMS, Cat# 17600), 1% lead citrate (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Developmental Biology 2023Quote: ... all WMISH fixations were done in 4% paraformaldehyde (PFA, stock 32% PFA ampules from Electron Microscopy Sciences, cat. 15714) in ASW for 6 h–overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... a 4% (v/v) paraformaldehyde (PFA) solution was diluted from 16% stock (Cat# 50-980-487, Electron Microscopy Sciences) by 1X Gibco™ DPBS (Cat# 14190-136 ...
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bioRxiv - Plant Biology 2024Quote: ... mix well and adjust pH with 9 mL 1N NaOH (Electron Microscopy Sciences, Cat. No. 21160, Hatfield, PA, USA). This solution was filtered with a 0.2 µm PES filter prior to use.
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bioRxiv - Physiology 2024Quote: ... Oil Red O staining was used to visualize lipid oil droplets in tissues (Electron Microscopy Sciences Cat #26609-01). Wet mount images of white adipose tissue were obtained after fixation in a 4% PFA solution prior to imaging and cell area and number measurements ...
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bioRxiv - Cell Biology 2024Quote: 1 × 106 cells in HBS were fixed using 4% paraformaldehyde (PFA) (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: For negative staining 5 µl of NLRP3 sample was placed on a copper grid (Electron Microscopy Sciences, cat. no: FCF400CU50), incubated for 1 min ...
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bioRxiv - Developmental Biology 2021Quote: ... as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu, EMS). After removal of gelatin by incubation on PBS at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells in FACS buffer (PBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences) for 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and mounted on the slide using 10 μl of FLUORO-GEL II mounting medium with DAPI (EMS; Cat.# 17985-51) and visualized using an Axio Observer Z1 fluorescence microscope (Zeiss) ...
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bioRxiv - Neuroscience 2021Quote: ... 100-nm thick sections were cut and collected on 300 hexagonal mesh copper grids (Electron Microscopy Sciences, cat # T300H-Cu). In each set of five grids ...
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bioRxiv - Neuroscience 2021Quote: ... Canada) diluted in water combined (1:1) with 4% aqueous osmium tetroxide (cat#19170, Electron Microscopy Sciences, Hatfield, PA, USA) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Molecular Biology 2019Quote: ... in PBS (+Ca2+/Mg2+) for 10 min and directly fixed afterwards with 2,4% paraformaldehyde (PFA; Electron Microscopy Sciences; cat.# 15710) in PBS (+Ca2+/Mg2+ ...
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bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Single β-cells from dispersed human islets were cultured on 25mm round coverslips from Electron Microscopy Sciences (cat# 72225-01) overnight in the human islet media describe above ...
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bioRxiv - Physiology 2020Quote: ... the PDMS was gently cut using a double edge carbon steel razor blade (Cat: 71933-50, Electron Microscopy Sciences, USA), placed onto double-sided tape that had been adhered to a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with ketamine/xylazine before transventricular cardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA; Cat #19210) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: 300 nm thick sections were collected on formvar-coated copper slot grids (Electron Microscopy Sciences, USA; Cat. No. GS2010-Cu) and stained with 2% uranyl acetate in 70% methanol followed by Reynold’s lead citrate (Mai et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
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bioRxiv - Systems Biology 2023Quote: Mouse hearts were briefly perfused with 10ml cold PBS and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Cat# 15710) (overnight ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... encapsulated cochleas were fixed for an hour in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (Electron Microscopy Sciences, Cat# 15960) supplemented with 2 mM CaCl2 ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Neuroscience 2023Quote: ... iAssembloids were first fixed with 4% paraformaldehyde (16% stock, diluted 1:4 in DPBS, Electron Microscopy Sciences, Cat. No. 15710) and then incubated in a 25% formamide (MilliporeSigma ...
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bioRxiv - Neuroscience 2023Quote: ... the resin-infiltrated sections were flat-embedded onto fluoropolymer films (ACLAR®, Pennsylvania, USA, Electron Microscopy Sciences, cat# 50425-25) covered with resin and kept at 55 °C in a convection oven for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... mice were perfused transcardially with heparinized saline and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... 1.1 ml was deposited on a glass slide and covered with a 5mm coverslip (cat. # 72296-05, Electron Microscopy Sciences). 11 images of each reaction were taken ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then immediately applied to glow-discharged Quantifoil grids (R 1.2/1.3, 400 mesh, copper grids, EMS Cat #: Q450CR1.3) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... A Leica VT1000 S vibratome was equipped with a platinum coated double edged blade (Electron Microscopy Sciences, Cat. #72003-01) and set to a maximal speed of 0.9mm/s for collecting 50 µm slices ...
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bioRxiv - Developmental Biology 2024Quote: 48 hpf Tg(kdrl:eGFP) and Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-Runx1-eGFP) embryos were fixed in 4% formaldehyde (Electron Microscopy Sciences, Cat#15712) diluted in PBS/0.1% tween20 (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were fixed for 15 min at RT with 4% electron microscopy grade paraformaldehyde (PFA; Electron Microscopy Sciences, cat. no. 15710) diluted in PIPES–EGTA– magnesium (PEM ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was removed and the neurons were fixed with 4% electron-microscopy-grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in a cytoskeleton-protective buffer (PEM ...
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bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were coverslipped with mounting medium (EMS Immuno Mount DAPI and DABSCO, Electron Microscopy Sciences, Cat # 17989-98, Lot 180418). For optogenetics animals ...
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bioRxiv - Neuroscience 2023Quote: ... Labeled sections were mounted onto glass slides and coverslipped with fluorescent mounting medium (Citofluor AF1; Cat#17970-25, Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2023Quote: ... The block edges were covered with additional strips of carbon tape and 2-4 strips of copper tape (EMS; cat#77802) and coated with a layer of colloidal silver liquid (EMS ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (10 µL) of fixed cells were then loaded onto PTFE printed glass slides (cat#63429-04 from Electron Microscopy Sciences) with 4-mm diameter wells and allowed to air-dry ...
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bioRxiv - Neuroscience 2023Quote: ... and slices were fixed right after recordings by immersion in freshly prepared 2.5% glutaraldehyde (from Electron Microscopy Science Cat. no. 16220), 4% paraformaldehyde (from SIGMA-ALDRICH Cat.no ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Cell Biology 2023Quote: ... the flies were anesthetized using CO2 gas and mounted to microscope slides using clear nail polish (Electron Microscopy Sciences, Cat# 72180) applied to the wing base and dorsal side of the thorax by an experimenter masked to genotype and treatment ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed at 10-15 min post-damage for 45 min with 4% paraformaldehyde (PFA; Cat No – RT-15710, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Biophysics 2019Quote: MCN were fixed from 15th to 19th day in vitro (DIV) with 4 % EM grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in PEM buffer (80 mM PIPES ...
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Tape containing sections was attached to a 4-inch silicon wafer in rows using double sided carbon tape (EMS, Cat# 77817-50) and sections were post-stained with uranyl acetate and lead citrate ...