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Citations for Electron Microscopy Sciences :
451 - 500 of 529 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The animals were intracardially perfused with 100 mL of 0.9% physiological saline followed by approximately 250 mL of freshly prepared 0.1 M sodium cacodylate buffer (pH 7.4; Electron Microscopy Sciences cat #11653), which contained 2.5% glutaraldehyde (Electron Microscopy Sciences cat #16320 ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were removed from the wells and mounted onto microscope slides using Fluorogel II with DAPI (cat. No. 17985-50, Electron Microscopy Sciences). Coverslips were imaged with confocal microscopy and the proliferation index was evaluated by dividing total cell number by EdU-positive cells.
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bioRxiv - Cell Biology 2022Quote: ... The tissue was dehydrated using a graded ethanol series and infiltrated with Epon-812 (Electron Microscopy Sciences, cat# RT 13940; Hatfield, PA) using propylene oxide as the transition solvent ...
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bioRxiv - Neuroscience 2020Quote: ... the separation of the brain from the VNC was performed using fine-tipped forceps and MicroTools (Cat #: 50-905-3315, Electron Microscopy Sciences). The time from digestion (the part of the protocol most likely to induce cell stress ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then dehydrated in acetone and incubated in a series of three progressively concentrated acetone-Epon 812 resin (EMS, Cat#RT14120) mixtures ...
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bioRxiv - Molecular Biology 2021Quote: ... Resuspended cells were loaded on Concanavalin A-treated coverslip (#1.5 Micro Coverglass −25 mm Diameter, Electron Microscopy Sciences, Cat. No. 72225-01) assembled on imaging cell chamber (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11, Electron Microscopy Science). The carbon layer was floated on the water surface and then transferred to the cryo-EM grids by raising them to the water surface under the carbon layer ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
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bioRxiv - Cell Biology 2023Quote: ... and a hydrophobic ring was drawn around the tissue sample using a Liquid Blocker Super PAP Pen (Electron Microscopy Sciences, Cat#71312). The sample was incubated overnight at 4°C with a blocking solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
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bioRxiv - Microbiology 2023Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the cornea and the lens were removed from the eyes and the eyecups were further fixed by immersion in 4 % paraformaldehyde (PFA, EMS Cat#15710) for 30 minutes (min ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was pipetted onto precoated Laminin coverslips at 150-170 μl on each coverslip (Cat#: 72298-01, Electron Microscopy Science), placed in 35 mm petri dishes ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were washed with DPBS and then fixed with 4% paraformaldehyde (diluted from a 16% solution; Electron Microscopy Sciences cat. no. 15710) for 15 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: Purified rAAV8 samples (human-produced empty and full, baculovirus-Sf9 produced empty and full) were applied to Lacey carbon Quantifoil grids (EMS Cat# Q225CR-06) and flash frozen ...
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bioRxiv - Neuroscience 2019Quote: ... brains were briefly rinsed in 2.5% potassium dichromate and placed in a solution of 2.5% potassium dichromate and 0.01% osmium tetroxide (Electron Microscopy Sciences; Cat# 19150; Hatfield, PA) for around 12 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips containing the homozygously expressing SNAP-NOIs cells were pre-fixed with 2.4% [w/v] formaldehyde (FA; Electron Microscopy Sciences; cat.# 15710) in 1x PBS (+Ca2+/Mg2+) ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... in 2.5% potassium dichromate and transferred to an admixture of 2.5% potassium dichromate and 0.01% osmium tetroxide (Electron Microscopy Sciences; Cat# 19150; Hatfield, PA) for 24 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Cell Biology 2022Quote: Cells plated on glass coverslips were processed for chemical fixation (CF) by incubation for 30 minutes at room temperature with 0.2% glutaraldehyde (Electron Microscopy Science, Cat.16220) and 2.5% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were suspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% monomeric formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29) ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Cell Biology 2023Quote: 70 nm ultramicrotome sections were cut using a Diatome Ultra 45° diamond knife and collected onto square 100 mesh copper grids (EMS, Cat# G100-Cu). Grids were post-stained in 1.2% uranyl acetate diluted in water for 4 min ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Cell Biology 2023Quote: Wandering third-instar larvae were dissected in ice cold Ca2+-free saline and fixed for 6 minutes in Bouin’s Fixative (Electron Microscopy Sciences, Cat# 15990). Dissections were washed several times with 1X PBS and then permeabilized with 1X PBS containing 0.1% Triton-X (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 70 nm sections were cut using Diatome Ultra 45° diamond knife and transferred onto square 100 mesh copper grids (EMS, Cat# G100-Cu). Grids were post-stained in Reynold’s lead citrate stain (EMS ...
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Developmental Biology 2019Quote: Mice were sacrificed according to ethical guidelines and inner ears were dissected out in cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... A 5 μL sample of the peak fraction was then added to glow discharged copper CF400-CU EM grids (cat. # 71150, Electron Microscopy Sciences, Hatfield, PA) for 1 min ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Microbiology 2022Quote: ... 40 µL of fixed parasite sample was added to 11 mm wells in PTFE printed microscope slides (Electron Microscopy Sciences, Cat No: 63422-11) and incubated for half an hour ...
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bioRxiv - Genetics 2022Quote: ... samples were washed 3x in 0.3% v/v PBT and subjected to a second sample fixation in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Genetics 2022Quote: ... adult female intestines were dissected out in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Developmental Biology 2019Quote: ... Ultra-thin sections of 100 nm thickness for TEM analysis were then cut using an ultramicrotome and stained with 33% methanolic uranyl acetate for 15 minutes and lead citrate (Electron Microscopy Sciences, cat # 22410) for 7 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with ketamine/xylazine and transcardially perfused with PBS followed by PFA (4% in PBS; Electron Microscopy Sciences, cat n°15700). Extracted brains were post-fixed (PFA 4% overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion was done with a prewash with 9.25% sucrose in distilled water and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion TwoTM Automated Pressure Perfusion System (Leica Biosystems ...