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Citations for Electron Microscopy Sciences :
101 - 150 of 953 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed for 10 min in either -20°C methanol or 4% paraformaldehyde (Electron Microscopy Sciences). Cells were blocked and permeabilized in a solution containing 1:1 Odyssey blocking buffer (LiCor)/PBS (Invitrogen ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... Guts were fixed overnight at 4 °C in PBS with 4% paraformaldehyde (PFA, Electron Microscopy Sciences #15710). Dissected brains and ventral nervous systems were fixed for 30 minutes in 4% PFA at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fixed at 4°C for 10 min in 4% paraformaldehyde (Electron Microscopy Sciences). The fixed cells were washed and resuspended in PBS containing 5% FBS ...
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bioRxiv - Zoology 2021Quote: ... sponge tissues were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020) and post-fixed for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... A thin layer of clear dental cement (C&B super-bond) and nail polish (Electron Microscopy Sciences) were applied for strengthening and to reduce light glare during imaging ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of freshly purified CORVET samples at a protein concentration of approximately 0.8 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Single β-cells from dispersed human islets were cultured on 25mm round coverslips from Electron Microscopy Sciences (cat# 72225-01) overnight in the human islet media describe above ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... ∼107 tachyzoites growing intracellularly in HFF cells in a T25 flask were incubated for 4 h at 37°C in 0.1% FBS DMEM growth medium containing either 2.5 mM ethyl methanesulphonate (EMS) at final concentration or the appropriate vehicle controls ...
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bioRxiv - Microbiology 2023Quote: ... The samples were prefixed overnight at 4°C in the mixture of 4% PFA (Electron Microscopy Sciences, 15710) and 2% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 20 minutes at 4°C and then fixed in 4% PFA (Electron Microscopy Sciences) for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... was deposited onto glow-discharged C-flat holey carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences). These grids were blotted for 6 seconds with a blot force of 15 at 100% humidity and 22 degrees C before being rapidly submerged into liquid ethane that had been cooled by liquid nitrogen for plunge freezing.
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bioRxiv - Cell Biology 2023Quote: ... The samples were then freeze substituted at - 90°C with 0.1% (w/v) uranyl acetate and 0.01% (w/v) tannic acid (EMS) in acetone for 6h using a Leica EM AFS2 with a processor Leica EM FSP ...
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bioRxiv - Bioengineering 2023Quote: Ten microliters of EVs were incubated on freshly glow-discharged C-flat Holey carbon grids (Electron Microscopy Sciences) for at least 10 min in a humid chamber ...
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bioRxiv - Developmental Biology 2024Quote: ... euthanized by an overdose of tricaine and fixed overnight at 4°C in 4% paraformaldehyde (PFA, EMS, 15710). Larvae were washed three times for 15 minutes each with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos prepared for immunostaining were fixed overnight at 4°C in 2% PFA (Electron Microscopy Sciences, Catalog #15710). Embryos were either embedded in 4% low-melt agarose/PBS + 0.1% Tween and cut into 200um sections using a Leica VT1200 or stained as intact whole-mounts ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then fixed for 20 min at 37°C with 4% paraformaldehyde (PFA; Electron Microscopy Science,15710) in cytoskeleton buffer (CB ...
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bioRxiv - Cell Biology 2024Quote: ... the pancreas was fixed overnight at 4 °C in 4% paraformaldehyde (Electron Microscopy Sciences 19202, Hatfield, PA, USA). The pancreas was paraffin-embedded and sectioned into 4μm thick slices by the Translational Pathology Core Laboratory at UCLA.
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bioRxiv - Microbiology 2021Quote: ... After that, Samples were infiltrated in graded mixtures (1:3, 1:1, 3:1) of resin (EMS, Resin Mixture ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Genetics 2019Quote: ... Subsequent analysis concentrated on the 1,854 transition variants (C to T or G to A) as these were likely caused by EMS mutagenesis ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Biochemistry 2021Quote: 4 µL of dialyzed sample was deposited onto glow-discharged C-flat Au 1.2/1.3 grids (Electron Microscopy Sciences), and flash frozen using a manual plunger ...
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bioRxiv - Biochemistry 2021Quote: ... 30 sec in air at 20 mA and 0.4 mbar) C-flat 1.2/1.3-3Au grids (Electron Microscopy Sciences), and blotted for 4 sec using a Vitrobot Mark IV (FEI ...
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bioRxiv - Neuroscience 2021Quote: One 5 mm sample from each naïve nerve was fixed for 48 hours at 4° C in a solution of 2% glutaraldehyde (16216, EMS), 3% paraformalydehyde (15754-S ...
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bioRxiv - Developmental Biology 2022Quote: ... and submerged in 200°C heated R-Universal Epitope Recovery Buffer solution (Electron Microscopy Sciences, Hatfield, PA, 62719-20) for 30 minutes and then let cool at room temperature for 25 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Cells were fixed for 20 min with a mixture of prewarmed (37°C) 0.25% glutaraldehyde (Electron Microscopy Sciences 16220) and 4% formaldehyde (Electron Microscopy Sciences 15714 ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat. no. 19100 Electron Microscopy Sciences) for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was discarded and the pellet was kept at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science) before analysis.
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently incubated with rotation at 4 °C first in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Cat: 15710-S) for 24 hours and then in 30% (w/v ...
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bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
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bioRxiv - Plant Biology 2024Quote: ... Samples of 2.5 µL were applied to a C-flat 300 mesh 1.2/1.3 copper grids (Electron Microscopy Sciences), glow-discharged in the presence of amylamine at 25 mA for 45 s ...
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bioRxiv - Developmental Biology 2024Quote: ... and fixed by immersion for at least one overnight at 4°C in 2.5% glutaraldehyde (Electron Microscopy Sciences; 16200) and 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Bioengineering 2024Quote: ... before being fixed in pre-warmed (5 min in 37° C water bath) 4% formaldehyde (Electron Microscopy Sciences, 15710) in PBS solution for 5 min and permeabilized in pre-warmed (to 37° C ...
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bioRxiv - Developmental Biology 2024Quote: Tissues were fixed between 15 minutes to 3 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Post-fixation samples were dehydrated with ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
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bioRxiv - Molecular Biology 2020Quote: Purified rAAV8 samples (human-produced empty and full, baculovirus-Sf9 produced empty and full) were applied to Lacey carbon Quantifoil grids (EMS Cat# Q225CR-06) and flash frozen ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of F-actin diluted to 0.6 μM in KMEI + NP40 was applied to a glow discharged C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica GP plunge freezer operating at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... wild-type 81116 strain cell culture grown to OD600 = 5 was applied onto glow-discharged C-flat holey carbon films 2/2 200 mesh (EMS). A Leica EM GP (Leica ...
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bioRxiv - Microbiology 2021Quote: ... 3.5 µl of the complex solution were deposited onto a C-flat 1.2/1.3-3C holey carbon copper grid (Electron Microscopy Sciences) that had been freshly glow-discharged for 1 min at 20 mA using a PELCO easiGLOW (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... then rinsed in 100% acetone and infiltrated over 24 h at −600°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at −450°C for 48 h ...
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bioRxiv - Biochemistry 2022Quote: A Gatan Solarus Model 950 advanced plasma system was used to clean C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400; Electron Microscopy Sciences). Settings used for the plasma cleaning were ‘Cleaning time’ ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of solution was applied to a plasma-cleaned C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...