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Citations for Electron Microscopy Sciences :
1 - 50 of 953 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL of the purified CVB5 mutant virus sample at 4.5 mg mL-1 concentration was loaded onto Quantifoil R 1.2/1.3 grids (EMS), that were previously glow-discharged for 30 s in a GloCube Plus device (Quorum Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μL of virus mixed with Fast Green FCF Solution 2.5% (Electron Microscopy Sciences, Hatfield, PA, USA). The mouse was placed on a heating blanket (37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... PCW17 and PCW21 human cortical tissue was fixed overnight at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Samples were then washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... or -20°C methanol with 1% paraformaldehyde (Electron Microscopy Sciences, 15710) for 10 minutes on ice ...
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bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
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bioRxiv - Cell Biology 2023Quote: ... and then post-fixed for 1 hr at 4°C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1 M Sodium Cacodylate ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Microbiology 2020Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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bioRxiv - Immunology 2023Quote: ... Cell post-fixation was as follows: 1 hour at 4°C with 1% osmium tetroxide (Electron Microscopy Sciences) and 0,8% potassium ferricyanide in PBS ...
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bioRxiv - Immunology 2020Quote: ... at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences, UK) and 1,5 % ferrocyanide (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... washed twice in PBS (23°C) and fixed in either 1% glutaraldehyde (EMS, 16020) prepared in BRB80 buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Cell Biology 2021Quote: ... Post-fixation was performed for 1 hour at 4 °C using 1 % osmium tetroxide and 0.8 % potassium ferracyanide (Electron Microscopy Sciences) in 0.1 mol/l cacodylate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.2 at 4° C and post-fixed with 1% osmium tetroxide (# 19150, Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer (#12300 ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... and filled with 0.5 µL of virus (titer ∼ 1012) dyed with Fast Green 2.5% (Electron Microscopy Sciences, Hatfield, PA, U.S.A.). The mice (∼8 weeks old ...
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bioRxiv - Microbiology 2019Quote: ... of purified protein at a concentration of 1 mg ml-1 was deposited onto glow-discharged C-flat holey carbon films 1.2/1.3 200 mesh (EMS). A Vitrobot Mark III (FEI ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 2h at room temperature and stored in 1% PFA in PBS at 4°C and finally fixed with 2.5% GA and 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM dithiothreitol (DTT) were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of the purified G6PDH-OpcA sample at a protein concentration of approximately 1 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and directly plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... This was polymerised for 24 h at 60°C inside a closed gelatin capsule (size 1; Electron Microscopy Sciences) half-filled with previously hardened resin to ensure the insect was situated in the center of the final resin block ...
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bioRxiv - Microbiology 2020Quote: Four microliters of purified virus were applied to a Formvar-and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
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bioRxiv - Microbiology 2021Quote: Four μL of purified virus was applied to a Formvar- and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Microbiology 2021Quote: ... approximately 4 μL of purified virus sample was applied to a glow-discharged 300-mesh copper grid coated with continuous carbon film (Electron Microscopy Sciences, Hatfield, PA). The grid was plunged into a liquid ethane container that is further cooled by a liquid nitrogen bath ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3 μl of the particle dispersion was applied on 400-mesh C-Flat CF-2/1-4C copper grids (Protochips, USA) negatively glow discharged with a Plasma Cleaner (EMS, USA) at 50 mA for 45 s ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were allowed to fix overnight at 4 °C and then post-stained with 1% aqueous osmium tetroxide (EMS, 19100) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Cell Biology 2024Quote: ... were immersed in and fixed by 4 % glutaraldehyde (48 h/4 °C), post-fixed by 1% osmium tetroxide (2 h, RT) and were embedded into Epon resin (Electron Microscopy Sciences) as described previously32 ...
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bioRxiv - Biochemistry 2021Quote: ... Quantifoil and C-flat grids (Electron Microscopy Sciences) were glow discharged with Pelco easiGlow for 60s at 25mA (negative polarity) ...
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bioRxiv - Immunology 2022Quote: ... 1.2/1.3 C-Flat grid (Electron Microscopy Sciences). After 20 s of incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and perfused transcardially with warm (~37 °C) Ames medium (MilliporeSigma) with heparin (MilliporeSigma) followed by warm (~37 °C) 2.5% glutaraldehyde (Electron Microscopy Sciences), 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4 °C in 4% paraformaldehyde (PFA) (EMS, 15710). Then they were washed with PBS-Tween20 (0.1% ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: Human neurons were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...