Labshake search
Citations for Electron Microscopy Sciences :
301 - 350 of 384 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...
-
bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
-
bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: Cells were seeded at 0,2 x 106 cells/mL in 24-well plates containing 12 mm round coverslips (#1.5 mm thickness, Electron Microscopy Science) with 1 mL per well and stimulated as described above ...
-
bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
-
bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.05 mg/mL) were applied on the carbon side of glow-discharged carbon-coated holey copper grids (EMS CF300-CU). After 1 min ...
-
bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
-
bioRxiv - Immunology 2023Quote: 3.5 µl of 70 µg/mL nanoparticles were applied to glow-discharged 400-mesh carbon-coated grids (Electron Microscopy Sciences) and stained with 2% (wt/vol ...
-
bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
-
bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
-
bioRxiv - Biophysics 2023Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.1 ml was deposited on a glass slide and covered with a 5mm coverslip (cat. # 72296-05, Electron Microscopy Sciences). 11 images of each reaction were taken ...
-
bioRxiv - Genomics 2019Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
-
bioRxiv - Biophysics 2020Quote: ... 3 μl microliters of freshly purified complexes (0.01 mg/ml) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and stained using 2% (w/v ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
-
bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
-
bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
-
bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
-
bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µl of DELE1 samples at 0.1 mg/ml were applied onto carbon coated 400 A° mesh Cu-Rh maxtaform grids (Electron Microscopy Sciences) which were plasma cleaned for 15 s using Pelco glow discharge cleaning system (Ted Pella ...
-
bioRxiv - Biophysics 2023Quote: ... one quarter of the bell from each medusae was chemically fixed in 10 mL of 2.5% [v/v] glutaraldehyde (Electron Microscopy Sciences), 4% [v/v] paraformaldehyde (Electron Microscopy Sciences) ...
-
bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Biochemistry 2023Quote: ... a 4 μl aliquot of the sample (diluted concentration: 0.25 mg/ml) was placed on ultra-thin carbon film grids (CF-200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
-
bioRxiv - Biochemistry 2023Quote: ... and 0.5 mg/mL) were incubated for 60 seconds on the carbon face of 200 mesh copper grids (Electron Microscopy Sciences) following their glow discharge at 25 mA for 30 seconds ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μL of purified proteins or immune complexes (∼0.02 mg/mL) were applied onto glow discharged (20 mA for 30s) carbon-coated 400 mesh copper grids (Electron Microscopy Sciences). After 5 s ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: Mice lungs were perfused with 8 mL PBS and inflated with 0.7 mL of OCT compound (Tissue-Tek) mixed 1:1 v/v with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in PBS ...
-
bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
-
bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.
-
bioRxiv - Biochemistry 2023Quote: ... 3 μL of spike-protein at 0.03 mg/ml in 1X TBS was deposited on a glow-discharged carbon coated copper mesh grid (Electron Microscopy Sciences) and blotted off ...
-
bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
-
bioRxiv - Molecular Biology 2020Quote: 3 µL of NT-NTR1-Gαi1β1γ1-cND complex at a concentration of 0.02 mg/mL was applied onto a glow-discharged continuous carbon grid (Electron Microscopy Sciences, Inc.). After two minutes of adsorption ...
-
bioRxiv - Immunology 2019Quote: ... Eight to 10 ml of blood was gently fixed and lysed by incubating with 792 µl of 16% paraformaldehyde (Electron Microscopy Sciences, Hatfield ...
-
bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
-
bioRxiv - Neuroscience 2021Quote: ... The animals were intracardially perfused with 100 mL of 0.9% physiological saline followed by approximately 250 mL of freshly prepared 0.1 M sodium cacodylate buffer (pH 7.4; Electron Microscopy Sciences cat #11653), which contained 2.5% glutaraldehyde (Electron Microscopy Sciences cat #16320 ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
-
bioRxiv - Microbiology 2020Quote: Complexes of M4H2K1 Fab and BG505 UFO.664 trimer were purified by SEC to remove unbound Fab and diluted to 0.01 mg/mL in Tris-buffered saline prior to adsorption onto carbon-coated and plasma cleaned copper mesh grids (Cu400, Electron Microscopy Sciences). Grids were stained with 2% (w/v ...