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Citations for Electron Microscopy Sciences :
251 - 300 of 384 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
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bioRxiv - Cell Biology 2022Quote: Four μm kidney cortices optimal cutting temperature (OCT) compound embedded sections were incubated with 100 μl freshly prepared Oil Red O solution (Electron Microscopy Science, Hatfield, PA) for 15 minutes and counterstained with Hematoxylin Harris solution (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... The latest dehydration steps performed with 100% ETOH and propylene oxide were followed by infiltration with epoxy resin (EMBed-812; Electron Microscopy Science, PA USA). Cochleae were cut with a Leica EM UC7 ultramicrotome ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Genetics 2023Quote: ... 5-10 μl of 1% PFA solution pH 9.2 containing 0.15% Triton X-100 and 3 mM DTT was placed in each well of a 12-well glass slide (Electron Microscopy Sciences, 63425-05) and one ZP-free oocyte was transferred to each well ...
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bioRxiv - Plant Biology 2020Quote: ... 100% of cold EtOH) prior to being resin infiltrated and embedded using the Lowicryl Monostep Embedding Media HM20 (#14345, Electron Microscopy Sciences, Hatfield, PA, USA) using either heat or UV polymerization ...
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bioRxiv - Pathology 2020Quote: ... glutaraldehyde for 1 h at room temperature then treated with 1% osmium tetroxide and dehydrated through a graded series of ethanol concentrations (from 30 to 100%) and embedding with an Embed 812 kit (Electron Microscopy Sciences, Fort Washington, PA). Ultrathin sections (60 to 80 nm ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Genomics 2021Quote: ... and the cells were fixed with 2 ml of 4% PFA in PBS (Electron Microscopy Sciences, 15710) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated brain tissue was fixed in 10 mL of 1% Methanol-free Paraformaldehyde (16%; Electron Microscopy Sciences) for 10 minutes before quenching with 0.25M Tris-HCl pH 8 (a more effective quencher than glycine [102]) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Biochemistry 2021Quote: ... the DACR clone #A4 at early passage was mutagenized again (0.3 mg/ml EMS for 72 h). After recovery ...
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bioRxiv - Microbiology 2021Quote: ... 50 ml of lake water was fixed with paraformaldehyde (final concentration 1%, without methanol, Electron Microscopy Sciences) overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 20 μl of whole blood was added to 1 ml of 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in Dulbecco’s PBS (DPBS – Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... and fixed in 20 ml of fixative containing 4% (v/v) paraformaldehyde (Electron Microscopy Sciences, Cat# 15710), 25% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... 50-µm or 120-µm coronal sections were transferred into 3.7 ml shell vials (Electron Microscopy Sciences) with 1 ml 1 x PBS ...
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bioRxiv - Physiology 2023Quote: ... stained with DAPI (1 mg/ml in PBS) and mounted with Fluorogel mounting medium (EMS, Hatfield, PA), Immunostaining was visualized by with a Zeiss LSM980 confocal microscope.
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of freshly purified CORVET samples at a protein concentration of approximately 0.8 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL of the purified CVB5 mutant virus sample at 4.5 mg mL-1 concentration was loaded onto Quantifoil R 1.2/1.3 grids (EMS), that were previously glow-discharged for 30 s in a GloCube Plus device (Quorum Technologies) ...
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bioRxiv - Neuroscience 2024Quote: Mice were perfused with 20 mL 1XPBS followed by 15mL of 4% PFA (Electron Microscopy Sciences # 15172) diluted in PHEM buffer (0.1 M ...
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bioRxiv - Neuroscience 2020Quote: ... After washing in HPLC water and dehydration through a four step ethanol series from 50% to 100% ethanol tissues were immersed in propylene oxide (Electron Microscopy Sciences; Cat# 20401; Hatfield, PA), and left for 15min ...
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bioRxiv - Microbiology 2019Quote: ... of purified protein at a concentration of 1 mg ml-1 was deposited onto glow-discharged C-flat holey carbon films 1.2/1.3 200 mesh (EMS). A Vitrobot Mark III (FEI ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
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bioRxiv - Biochemistry 2022Quote: 3 µl of ~ 1.5 µM (0.75 mg/ml) of the complex was applied to freshly glow discharged quantifoil 300 mesh R 1.2/1.3 grids (EMS) and plunge frozen into liquid ethane using a Thermo Fisher Vitrobot Mark IV with conditions of 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of 0.6–0.9 mg/ml of freshly purified wt HOPS complex were applied onto glow-discharged CF grids (R1.2/1.3) (EMS) and immediately plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 10 ml of 4% paraformaldehyde in DEPC-PBS (PFA, from paraformaldehyde 32% solution, Electron Microscopy Sciences). After perfusion ...
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bioRxiv - Biophysics 2023Quote: Purified NP at 0.01 mg/mL was applied to glow-discharged carbon-coated copper grids (Electron Microscopy Sciences) and stained with uranyl acetate immediately before imaging ...
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bioRxiv - Immunology 2023Quote: ... complexes were diluted to 0.01 mg/mL and immediately applied to EM grids (Electron Microscopy Sciences #CF200-Cu). Grids were then incubated for one minute ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of the purified G6PDH-OpcA sample at a protein concentration of approximately 1 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and directly plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Temporal bones were immediately removed and fixed via perfusion of 1 ml cold 4% PFA (Electron Microscopy Science) in 1X PBS through the oval and round windows ...
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bioRxiv - Biophysics 2021Quote: Samples described above were diluted in Buffer C and deposited onto freshly glow-discharged (EMS-100 Glow Discharge System, Electron Microscopy Sciences, 30 s at 15 mA) copper Quantifoil (Quantifoil Micro Tools GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...
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bioRxiv - Neuroscience 2021Quote: ... followed by adding ddH2O up to a final volume of 30 mL after accounting for glutaraldehyde (Electron Microscopy Science) volume for final BSA concentrations equal to ...
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bioRxiv - Cell Biology 2019Quote: ... homozygous pans1-1 seeds were incubated for 17 h at room temperature in 5 mL of 0.3% (v/v) ethyl methanesulfonate (EMS) (SIGMA ...
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bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
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bioRxiv - Microbiology 2021Quote: ... 4 ml of cell suspension was spun down at 10000 g and pellets were fixed in glutaraldehyde solution 2.5% (EMS) and in osmium tetroxide 1% (EMS ...
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bioRxiv - Physiology 2019Quote: ... hPCLS were washed with PBS (3X 1ml) and fixed in 1 ml of 4% PFA (EMS EM grade#5710) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences, cat. #15714S, diluted in PBS) and incubated at 4°C for 15 minutes with rocking ...
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bioRxiv - Bioengineering 2020Quote: ... Purified complexes were deposited at approximately 0.003 mg/ml onto carbon-coated copper grids (EMS, Hatfield, PA, United States) for 2 mins ...
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bioRxiv - Biophysics 2023Quote: 3 µL of MRS2 (0.02 mg/mL) was applied on a glow discharged carbon-coated 400 square mesh copper grid (EMS) and incubate for 1 min ...
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bioRxiv - Immunology 2023Quote: 3 μL sample at 1.8 mg/ml was applied to a glow-discharged Quantifoil R1.2/1.3 400 mesh gold grid (Electron Microscopy Sciences), blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Plant Biology 2024Quote: ... mix well and adjust pH with 9 mL 1N NaOH (Electron Microscopy Sciences, Cat. No. 21160, Hatfield, PA, USA). This solution was filtered with a 0.2 µm PES filter prior to use.
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bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
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bioRxiv - Molecular Biology 2020Quote: ... we spread 5×104 protoplasts onto a regeneration plate and subjected them to a treatment of either 6 sec of 15 W UV or 12 μg/ml ethyl methanesulfonate (EMS) to cause random mutations in the genomes ...
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bioRxiv - Microbiology 2021Quote: ... Nanoparticle samples were diluted to ∼0.02 mg/ml and added onto carbon-coated copper 400 mesh grids (Electron Microscopy Sciences) that had been plasma cleaned for 10 s with Ar/O2 ...