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Citations for Electron Microscopy Sciences :
401 - 450 of 458 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... a 3.5 μL drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) with a 4 nm carbon coat ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The 40 μm cryosections were cut at -18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were placed on a Durcupan block with a flat surface and covered with ACLAR® 33 C Film (Electron Microscopy Science). Samples were let polymerize for 72 h at 60 °C.
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µl of each sample was applied to the plasma-cleaned C-flat grids (Electron Microscopy Sciences, CF-1.2/1.3-4Au-50), pre-blotted and blotted with filter paper (Whatman 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... Three to four microliters of rsVLP or rsVLP-compound-GSH mixture were applied to either glow-discharged C-flatTM holey carbon copper grids (product No. CF312, Electron Microscopy Sciences) for the PV1-SC6b yeast sample or Lacey carbon copper grids with an ultra-thin carbon support film (product No ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature (25°C) with 4% PFA (Electron Microscopy Sciences) diluted in PBS and stored at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4 buffer for P116 without the N-terminal domain (246–818)) was applied to a 45 s glow- discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Biophysics 2024Quote: ... 200 mM NaCl and 0 – 0.01% LMNG or 1 mM CHAPSO) was applied to a 45 s glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) or R1.2/1.3 300 copper mesh quantifoil grid (Quantifoil Micro Tools ...
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bioRxiv - Biochemistry 2024Quote: CryoEM grids were prepared by applying 3.5 μL of 0.6 mg/mL NX11Q21.5-DNA48 in TBS with 2.5% glycerol to glow-discharged 400 mesh C-flat grids (CF-2/2-4C, 2 μm holes, 2 μm spacing; Electron Microscopy Sciences). The grids were blotted with a blot force of 0 for 6.5 seconds at 100% humidity and 20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of the sample was repeatedly applied and manually blotted three times onto 300 mesh R1.2/R1.3 C-flat Holey carbon grids (Electron Microscopy Sciences, Hatfield, PA, USA) using the multiple blotting technique prior to the Vitrobot step to increase the concentration of pili (34) ...
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bioRxiv - Biochemistry 2022Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) or Quantifoil R2/2 Cu 300 mesh grids in the chamber of the Leica GP2 plunging robot ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM samples of both EVs and LUVs were prepared on either lacey carbon or C – flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm Protein A colloidal gold particles (Au – NP ...
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bioRxiv - Neuroscience 2022Quote: ... The 40 μm cryosections were cut at −18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences 71314) and stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded acetone series and embedded for 72 hours at 60°C in Embed-812 resin (EMS, Hatfield, PA, USA). Ultrathin sections were collected on copper grids ...
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bioRxiv - Biochemistry 2021Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) in the chamber of the Leica GP2 plunging robot ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2024Quote: ... The filters were then removed from the culture dishes and incubated overnight in 4% paraformaldehyde solution at 4 °C (Electron Microscopy Sciences #15713). In preparation for paraffin embedding and sectioning ...
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bioRxiv - Biophysics 2024Quote: Cryo-EM samples of concentrated peptide solutions were prepared on either 300 mesh 2/2 Quantifoil or C-flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm protein A-conjugated colloidal gold particles (Au–NP ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Developmental Biology 2024Quote: Hearts were collected in 1X PBS (phosphate-buffered saline) at 4°C and fixed in 4% aqueous buffered paraformaldehyde in PBS (PFA, Electron Microscopy Sciences, 15714) overnight ...
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bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Biophysics 2021Quote: ... Cochleae from C57BL/6J wild-type mice at P6 and P14 were fixed overnight at 4°C in 4% PFA (Electron Microscopy Sciences, Hatfield, PA) in 1x PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Physiology 2024Quote: ... The brain was removed from the skull and a ∼6 mm thick section collected from the optic chiasm to the mamillary bodies using a rat coronal brain matrix (Electron Microscopy Sciences, 69083-C). The hypothalamus was isolated by removing brain matter above the top of the third ventricle and lateral to the optic tract ...
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bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.