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Citations for Electron Microscopy Sciences :
351 - 400 of 458 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 3.5 µl of the complex solution were deposited onto a C-flat 1.2/1.3-3C holey carbon copper grid (Electron Microscopy Sciences) that had been freshly glow-discharged for 1 min at 20 mA using a PELCO easiGLOW (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... then rinsed in 100% acetone and infiltrated over 24 h at −600°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at −450°C for 48 h ...
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bioRxiv - Biochemistry 2022Quote: A Gatan Solarus Model 950 advanced plasma system was used to clean C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400; Electron Microscopy Sciences). Settings used for the plasma cleaning were ‘Cleaning time’ ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of solution was applied to a plasma-cleaned C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain and pituitary were taken and fixated overnight at 4 °C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, Pennsylvania) diluted with phosphate buffered saline with Tween (PBST ...
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bioRxiv - Immunology 2020Quote: ... were loaded onto a glow-discharged C-flat grid (CF-1.2/1.3 400-mesh copper-supported holey carbon, Electron Microscopy Sciences), blotted for 4–5 s under 100% humidity at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... was subsequently applied on a glow-discharged C-flat CF-1.2/1.3 400 mesh holey carbon grids (Electron Microscopy Sciences), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were fixed overnight at 4°C in freshly prepared phosphate buffer (0.1 M, pH 7.4) containing 4% paraformaldehyde (15700, Electron Microscopy Sciences) and 0.2% glutaraldehyde (16200 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: Three microliters of the freshly purified complex were applied to a plasma-cleaned C-flat holy carbon grid (1.2/1.3, 400 mesh, Electron Microscopy Sciences) with 20 s glow discharge using air and flash-frozen in liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Three washes with cold acetone were carried out at 0°C and samples were embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60 °C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then fixed during confinement for 60 min at 37°C with 4% paraformaldehyde (PFA; Electron Microscopy Science,15710) in CB to allow fixative to penetrate the PDMS cell confiner ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were removed from the-80°C freezer and placed immediately into ice cold 4% PFA (Electron Microscopy Sciences, #15714)/PBS (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Microbiology 2023Quote: ... then rinsed in 100% acetone and infiltrated over 24 h to -45°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at -45°C for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Microbiology 2024Quote: ... Carbon-coated copper grids (200 mesh) and C-flat cryo-EM grids were purchased from Electron Microscopy Sciences (Hatfield, PA). Lacey carbon cryo grids and 2% (w/w ...
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bioRxiv - Physiology 2024Quote: ... slides were heated for 30 minutes at 65°C followed by submersion in Histoclear II (Electron Microscopy Sciences 64111-04) twice for five minutes ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated adult eyes were fixed for 1 hour at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... animals were transcardially perfused with 20 mL of ∼37°C PBS 0.1M followed by 20 mL of ice-cold 4% paraformaldehyde (EMS, 15714S) in PBS 0.1M ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were fixed for 2 hours at room temperature or overnight at 4° C in 4% paraformaldehyde (EMS catalog # 15710) and 4% sucrose (MP Biomedicals catalog # 802536 ...
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bioRxiv - Cell Biology 2020Quote: Cells were cultured on cell tight C-2 cell disks (Sumitomo Bakelite) and fixed for 2 h in 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples were immersion-fixed for at least one overnight at 4°C in 2.5% glutaraldehyde + 4% paraformaldehyde (Electron Microscopy Science) in a 1mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Cell Biology 2021Quote: ... Post-fixation was performed for 1 hour at 4 °C using 1 % osmium tetroxide and 0.8 % potassium ferracyanide (Electron Microscopy Sciences) in 0.1 mol/l cacodylate buffer ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of sample was loaded on freshly glow discharged 1.2-1.3 300 mesh C-flat™ grids without further support (Electron Microscopy Sciences). The Vitrobot chamber was set to 4 °C and 95% humidity and grids were prepared with a range of blotting time and force ...
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bioRxiv - Cancer Biology 2022Quote: ... Target retrieval was performed in citrate buffer at pH 6.0 for 15 minutes at 110°C under pressure and sections were transferred to Sequenza slide staining racks (Electron Microscopy Sciences). Sections were treated with Bloxall (Vector labs ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Microbiology 2022Quote: Bacteria were grown in Luria Broth liquid culture overnight at 37°C and immobilized on a Formvar grid (Electron Microscopy Sciences) for 1 min 30 sec and the samples were negatively stained with 0.5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4 buffer) was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... From this stock a 3.5 µl drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Developmental Biology 2023Quote: ... as well as P14 Hmgb1flx/flx and Hmgb1ΔAstro animals was extracted and fixed overnight at 4°C in 4% RNase-free PFA (Electron Microscopy Sciences) in sterile PBS ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of concentrated pseudovirus sample was applied to a carbon-coated grid (2/2-4C C-flats; Electron Microscopy Sciences), blotted with filter paper ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Plant Biology 2023Quote: ... The specimens were finally polymerised at 70°C for 72 h in flat embedding silicon molds (#70905-01, EMS, Hatfield, USA).
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Microbiology 2023Quote: ... and the transwell insert was washed with Dulbecco’s phosphate-buffered saline (DPBS) and fixed overnight at 4°C with cold 4% paraformaldehyde (PFA; Electron Microscopy Sciences) diluted in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The testes were extracted and fixed for two days at 4 °C in 4 % PFA (Electron Microscopy Sciences, Hatfield, PA ,USA). Samples were then washed with PBS for at least two days ...