Labshake search
Citations for Electron Microscopy Sciences :
401 - 450 of 3155 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos prepared for immunostaining were fixed overnight at 4°C in 2% PFA (Electron Microscopy Sciences, Catalog #15710). Embryos were either embedded in 4% low-melt agarose/PBS + 0.1% Tween and cut into 200um sections using a Leica VT1200 or stained as intact whole-mounts ...
-
Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
-
bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
-
bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
-
bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
-
bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
-
bioRxiv - Plant Biology 2023Quote: ... nrpe1-1 (EMS), and drm1-2 (SALK_031705 ...
-
bioRxiv - Cell Biology 2023Quote: ... containing 0.1% tanic acid (EMS),1% glutaraldehyde (EMS) in anhydrous acetone (EMS ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
-
bioRxiv - Cell Biology 2020Quote: ... atgl-1(xd310) and atgl-1(xd314) were generated by EMS. xdIs182 (Prab-3::Cre/Podr-1::RFP ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were then embedded in 1:1 Embed-812 (EMS 14120):acetonitrile ...
-
bioRxiv - Microbiology 2023Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
-
bioRxiv - Microbiology 2024Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
-
bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
-
bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
-
bioRxiv - Biophysics 2024Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
-
bioRxiv - Immunology 2024Quote: ... cells were washed with PBS twice followed by fixation and permeablization in 1 ml PBS containing 2% PFA (Electron Microscopy Sciences) and 0.1% saponin (Sigma–Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... were mixed with 0.5 µl of 0.04 mM Lauryl maltose neopentyl glycol (LMNG) solution immediately before sample deposition onto QUANTIFOIL® 2/1 400 mesh grids (EMS), which were plasma cleaned for 10 s using Gatan Solarus 950 Plasma system (Ar/O2 gas mixture) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
-
bioRxiv - Neuroscience 2021Quote: One 5 mm sample from each naïve nerve was fixed for 48 hours at 4° C in a solution of 2% glutaraldehyde (16216, EMS), 3% paraformalydehyde (15754-S ...
-
bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat. no. 19100 Electron Microscopy Sciences) for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then moved to a 1:1 solution of liquid nitrogen and 1% uranium acetate (Electron Microscopy Sciences, 22400) before undergoing freeze substitution and low temperature embedding (Leica ...
-
bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... at the time of brain microdissection the spinal cord was rapidly dissected and immersed in 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA). For assessment of phospho-cJUN and ATF3 expression animals were perfused with 4% PFA ...
-
bioRxiv - Neuroscience 2024Quote: ... i.p.) and transcranial perfused with 1 × ice-cold phosphate-buffered saline (PBS) followed by 4% ice-cold paraformaldehyde (PFA, Electron Microscopy Sciences, Pennsylvania, USA) made in PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... and then post-fixed for 1 hr in 1% osmium tetroxide (EMS) in 0.1 M sodium cacodylate ...
-
Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
-
bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
-
bioRxiv - Biophysics 2021Quote: ... 2uL of dialyzed sample was then applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 200- or 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were subsequently blotted for 2 or 3 seconds ...
-
bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
-
bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Pathology 2021Quote: ... slides were washed twice with 1 mL of PBS wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
-
bioRxiv - Neuroscience 2021Quote: ... was applied to glow-discharged Quantifoil holey carbon grids (Gold R2/1; 200 mesh) overlaid with an additional 2-nm carbon layer (Electron Microscopy Sciences). The grids were blotted for 3-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Genomics 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
-
bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
-
bioRxiv - Biophysics 2024Quote: ... These cells in the log-growth phase were collected and deposited on glow-discharged Quantifoil grids (R 2/1, Cu 200-mesh grid, Electron Microscopy Sciences), as described previously (5) ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...