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Citations for Electron Microscopy Sciences :
301 - 350 of 3155 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... for 2 days at room temperature (peritoneal cavity) or in 4% paraformaldehyde (Electron Microscopy Sciences) overnight at 4 °C (muscle tissue).
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bioRxiv - Cell Biology 2024Quote: ... specimens underwent post-fixation with 4% osmium tetroxide and 2% potassium ferrocyanide (Electron Microscopy Sciences) for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... placed on Maxtaform copper/rhodium 2×1 mm slot grids coated with 0.8% formvar (Electron Microscopy Sciences), post-stained 1% aqueous uranyl acetate (Polysciences ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2% osmium tetroxide and then stained overnight in 1% uranyl acetate (Electron Microscopy Sciences, 22400). The final stain was at 50 °C ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... before being post-fixed with osmium-ferricyanide (2 % OsO4, Electron Microscopy Sciences, Cat. # 19100; 1% K3Fe(III)(CN)6 ...
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bioRxiv - Immunology 2020Quote: Pyroptotic BMDMs were fixed for 1 hour at room temperature (RT) in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15710), then incubated with PBS containing 50 mM NH4Cl (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with ddH2O three times 5 min and incubated in 1% thiocarbohydrazide (EMS, cat# 2231-57-4) diluted in ddH2O for 20 min ...
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bioRxiv - Cell Biology 2024Quote: 1 × 106 cells in HBS were fixed using 4% paraformaldehyde (PFA) (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... was performed by placing 5 μL drops of the bacteria suspension for 3 minutes directly on glow discharged carbon coated grids (EMS). The grids were then washed with two drops of 2% aqueous uranyl acetate ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... the flame sealed end of the tubing was immediately dipped into a parlodion solution (∼3 cm2 parlodion, Electron Microscopy Sciences [Hatfield ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed for at least 2 hours (at room temperature) to overnight (at 4° C) in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in artificial sea water ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells in FACS buffer (PBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The grid was quickly washed using a 4-μL drop of milli-Q (MilliporeSigma) water and stained with 4 μL of negative stain solution composed of either 1% (w/v) uranyl acetate (EMS) with 0.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: Adult animals were perfused with 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA), brains ...
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bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Immunology 2024Quote: Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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bioRxiv - Bioengineering 2024Quote: ... mice were perfused transcardially with heparinized saline (10 units mL−1 of heparin) and 4% paraformaldehyde (PFA) that was freshly prepared from 32% PFA aqueous solution (Cat# 15 714, EMS), using a peristaltic pump at a rate of 7 mL min−1 ...
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bioRxiv - Genetics 2020Quote: ... sections were mounted with Fluoromount-G containing 4′,6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences). Staining was visualized and imaged using a Nikon 80i microscope with a Nikon DS-Fi1 camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed in 4% paraformaldehyde and 2% sucrose in PBS for 10 mins (EMS Diasum). Fixed cells were permeabilized/blocked in 0.2% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... sponges were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020), post-fixed for 1 hour at room temperature with 1% OsO4 (Electron Microscopy Science ...
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bioRxiv - Immunology 2024Quote: ... washed again with PBS + 2% FBS and fixated in PBS + PFA (4:100, Electron Microscopy Sciences).
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...