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Citations for Electron Microscopy Sciences :
301 - 350 of 469 citations for 6 chloro 9H 3 C methyl β D ribofuranosyl purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... At 10.5 dpc the mice were sacrificed by cervical dislocation and embryos were fixed for 3 hours in 4% paraformaldehyde (PFA, EMS). Immunofluorescence on sections was performed as follows ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Physiology 2022Quote: ... they were rinsed 3 times 5 minutes in PB buffer and then postfixed by a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed 3 × 5 min in PB and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% potassium ferrocyanide in PB buffer for 2 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of F-actin diluted to 0.6 μM in KMEI + NP40 was applied to a glow discharged C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica GP plunge freezer operating at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... wild-type 81116 strain cell culture grown to OD600 = 5 was applied onto glow-discharged C-flat holey carbon films 2/2 200 mesh (EMS). A Leica EM GP (Leica ...
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bioRxiv - Microbiology 2021Quote: ... 3.5 µl of the complex solution were deposited onto a C-flat 1.2/1.3-3C holey carbon copper grid (Electron Microscopy Sciences) that had been freshly glow-discharged for 1 min at 20 mA using a PELCO easiGLOW (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... then rinsed in 100% acetone and infiltrated over 24 h at −600°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at −450°C for 48 h ...
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bioRxiv - Biochemistry 2022Quote: A Gatan Solarus Model 950 advanced plasma system was used to clean C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400; Electron Microscopy Sciences). Settings used for the plasma cleaning were ‘Cleaning time’ ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of solution was applied to a plasma-cleaned C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain and pituitary were taken and fixated overnight at 4 °C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, Pennsylvania) diluted with phosphate buffered saline with Tween (PBST ...
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bioRxiv - Immunology 2020Quote: ... were loaded onto a glow-discharged C-flat grid (CF-1.2/1.3 400-mesh copper-supported holey carbon, Electron Microscopy Sciences), blotted for 4–5 s under 100% humidity at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... was subsequently applied on a glow-discharged C-flat CF-1.2/1.3 400 mesh holey carbon grids (Electron Microscopy Sciences), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were fixed overnight at 4°C in freshly prepared phosphate buffer (0.1 M, pH 7.4) containing 4% paraformaldehyde (15700, Electron Microscopy Sciences) and 0.2% glutaraldehyde (16200 ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were removed from the-80°C freezer and placed immediately into ice cold 4% PFA (Electron Microscopy Sciences, #15714)/PBS (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Microbiology 2023Quote: ... then rinsed in 100% acetone and infiltrated over 24 h to -45°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at -45°C for 48 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Three washes with cold acetone were carried out at 0°C and samples were embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60 °C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then fixed during confinement for 60 min at 37°C with 4% paraformaldehyde (PFA; Electron Microscopy Science,15710) in CB to allow fixative to penetrate the PDMS cell confiner ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: Three microliters of the freshly purified complex were applied to a plasma-cleaned C-flat holy carbon grid (1.2/1.3, 400 mesh, Electron Microscopy Sciences) with 20 s glow discharge using air and flash-frozen in liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...