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Citations for Electron Microscopy Sciences :
201 - 250 of 469 citations for 6 chloro 9H 3 C methyl β D ribofuranosyl purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... main inflorescences (not side branches) were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2023Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... The void peaks from each purification were frozen similarly at the eluted concentration after dilution 1:1 with 6 nm BSA-tracer gold (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2021Quote: ... GUVs were placed on a 6 mm diameter chamber made from a silicon sheet using a core sampling tool (EMS #69039-60) and imaged prior to protein addition to ensure proper GUV formation and suspension ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 M glucose) and placed on 6 mm diameter chambers made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5 mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS, 69039-60). The silicon chamber was mounted on a 1.5-mm clean cover glass (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
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bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
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bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
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bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... plated in glass bottom dishes or hESC-derived striatal neurons on coverslips were washed in D-PBS and fixed for 10 min in 4% PFA (Electron Microscopy Sciences). The cells were washed 3 times with D-PBS and incubated with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: Cells seeded in 24-well plates on poly-D-Lysine-coated glass coverslips were washed once in PBS and fixed with 4 % methanol-free PFA (Electron Microscopy Science) for 15 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
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bioRxiv - Cell Biology 2023Quote: Wandering third-instar larvae were dissected in ice cold Ca2+-free saline and fixed for 6 minutes in Bouin’s Fixative (Electron Microscopy Sciences, Cat# 15990). Dissections were washed several times with 1X PBS and then permeabilized with 1X PBS containing 0.1% Triton-X (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Samples in resin were polymerized at 65°C for 36 hours in BEEM capsules (Electron Microscopy Sciences). Silver (~80 nm thick ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed for 10 min in either -20°C methanol or 4% paraformaldehyde (Electron Microscopy Sciences). Cells were blocked and permeabilized in a solution containing 1:1 Odyssey blocking buffer (LiCor)/PBS (Invitrogen ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... Guts were fixed overnight at 4 °C in PBS with 4% paraformaldehyde (PFA, Electron Microscopy Sciences #15710). Dissected brains and ventral nervous systems were fixed for 30 minutes in 4% PFA at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fixed at 4°C for 10 min in 4% paraformaldehyde (Electron Microscopy Sciences). The fixed cells were washed and resuspended in PBS containing 5% FBS ...
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bioRxiv - Zoology 2021Quote: ... sponge tissues were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020) and post-fixed for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... A thin layer of clear dental cement (C&B super-bond) and nail polish (Electron Microscopy Sciences) were applied for strengthening and to reduce light glare during imaging ...
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bioRxiv - Cell Biology 2023Quote: ... and then post-fixed for 1 hr at 4°C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1 M Sodium Cacodylate ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of freshly purified CORVET samples at a protein concentration of approximately 0.8 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times with PBS and then incubated with a 4% PFA solution (Electron microscopy sciences) for 30 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Biophysics 2022Quote: 3 µL of sample was pipetted onto Quantifoil R1.2/R1.3 holey carbon grids (Cu 400, Electron Microscopy Sciences) which had been glow discharged for 1 min using a PELCO easiGlow glow discharge cleaning system (Ted Pella) ...
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bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were then transferred to a 3:1 mixture of propylene oxide: Araldite resin (EMS, Cat#13940) for overnight incubation under gentle rolling at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times with 1X PBS and mounted on glass slides using Hydromount (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.